Perelman School of Medicine at the University of Pennsylvania

Ostap Lab

Recent Publications

For a complete list of Ostap Laboratory publications from PubMed, click HERE.

Cega: a single particle segmentation algorithm to identify moving particles in a noisy system. 
Masucci EM, Relich PK, Ostap EM, Holzbaur ELF, Lakadamyali M. 
Mol Biol Cell. 2021 Apr 19;32(9):931-941. doi: 10.1091/mbc.E20-11-0744. 
Epub 2021 Mar 31. PMID: 33788586; PMCID: PMC8108521. 

Myosin with hypertrophic cardiac mutation R712L has a decreased working stroke which is rescued by omecamtiv mecarbil
Snoberger A, Barua B, Atherton JL, Shuman H, Forgacs E, Goldman YE, Winkelmann DA, Ostap EM. 
Elife. 2021 Feb 19;10:e63691. doi: 10.7554/eLife.63691.
PMID: 33605878; PMCID: PMC7895523.

The mechanochemistry of the kinesin-2 KIF3AC heterodimer is related to strain- dependent kinetic properties of KIF3A and KIF3C.
Bensel BM, Woody MS, Pyrpassopoulos S, Goldman YE, Gilbert SP, Ostap EM. 
Proc Natl Acad Sci U S A. 2020 Jul 7;117(27):15632-15641. doi: 10.1073/pnas.1916343117.
Epub 2020 Jun 22. PMID:32571914; PMCID: PMC7354928.

Modulation of Kinesin's Load-Bearing Capacity by Force Geometry and the Microtubule Track. 
Pyrpassopoulos S, Shuman H, Ostap EM.
Biophys J. 2020 Jan 7;118(1):243-253. doi: 10.1016/j.bpj.2019.10.045.
Epub 2019 Dec 12. PMID: 31883614; PMCID: PMC6952184. 

The regulatory protein 14-3-3β binds to the IQ motifs of myosin-IC independent of phosphorylation.
Ji HH, Ostap EM. 
J Biol Chem. 2020 Mar 20;295(12):3749-3756. doi: 10.1074/jbc.RA119.011227. 
Epub 2019 Dec 6. PMID: 31811090; PMCID: PMC7086031.

Single molecule mechanics resolves the earliest events in force generation by cardiac myosin. 
Woody MS, Winkelmann DA, Capitanio M, Ostap EM, Goldman YE.
Elife. 2019 Sep 17;8:e49266. doi: 10.7554/eLife.49266.
PMID: 31526481; PMCID: PMC6748826

Molecular to organismal chirality is induced by the conserved myosin 1D. 
Lebreton G, Géminard C, Lapraz F, Pyrpassopoulos S, Cerezo D, Spéder P, Ostap EM, Noselli S. 
Science. 2018 Nov 23;362(6417):949-952. doi: 10.1126/science.aat8642. 
PMID: 30467170; PMCID: PMC6698710.

High-resolution cryo-EM structures of actin-bound myosin states reveal the mechanism of myosin force sensing.
Mentes A, Huehn A, Liu X, Zwolak A, Dominguez R, Shuman H, Ostap EM, Sindelar CV.
Proc Natl Acad Sci U S A. 2018 Feb 6;115(6):1292-1297. doi: 10.1073/pnas.1718316115. Epub 2018 Jan 22.

High-resolution cryo-EM structures of actin-bound myosin states reveal the mechanism of myosin force sensing.
Mentes A, Huehn A, Liu X, Zwolak A, Dominguez R, Shuman H, Ostap EM, Sindelar CV.
Proc Natl Acad Sci U S A. 2018 Feb 6;115(6):1292-1297. doi: 10.1073/pnas.1718316115. Epub 2018 Jan 22.

Opposing Kinesin and Myosin-I Motors Drive Membrane Deformation and Tubulation along Engineered Cytoskeletal Networks.
McIntosh BB, Pyrpassopoulos S, Holzbaur ELF, Ostap EM.
Curr Biol. 2018 Jan 22;28(2):236-248.e5. doi: 10.1016/j.cub.2017.12.007. Epub 2018 Jan 11.

Adhesion force and attachment lifetime of the KIF16B-PX domain interaction with lipid membranes.
Pyrpassopoulos S, Shuman H, Ostap EM.
Molecular biology of the cell. 2017; 28(23):3315-3322.
PubMed [journal] PMID: 28931594 

Deconvolution of Camera Instrument Response Functions.
Lewis JH, Jamiolkowski RM, Woody MS, Ostap EM, Goldman YE.
Biophysical journal. 2017; 112(6):1214-1220.
PubMed [journal] PMID: 28355548 PMCID: PMC5376110

Measuring the Kinetic and Mechanical Properties of Non-processive Myosins Using Optical Tweezers.
Greenberg MJ, Shuman H, Ostap EM.
Methods in molecular biology (Clifton, N.J.). 2017; 1486:483-509. NIHMSID: NIHMS873378
PubMed [journal] PMID: 27844441 PMCID: PMC5506543

Activity-Dependent Regulation of Distinct Transport and Cytoskeletal Remodeling Functions of the Dendritic Kinesin KIF21B.
Ghiretti AE, Thies E, Tokito MK, Lin T, Ostap EM, Kneussel M, Holzbaur ELF.
Neuron. 2016; 92(4):857-872. NIHMSID: NIHMS827672
PubMed [journal] PMID: 27817978 PMCID: PMC5283298

MEMLET: An Easy-to-Use Tool for Data Fitting and Model Comparison Using Maximum-Likelihood Estimation.
Woody MS, Lewis JH, Greenberg MJ, Goldman YE, Ostap EM.
Biophysical journal. 2016; 111(2):273-282.
PubMed [journal] PMID: 27463130 PMCID: PMC4968482

Myosin-I molecular motors at a glance.
McIntosh BB, Ostap EM.
Journal of cell science. 2016; 129(14):2689-95.
PubMed [journal] PMID: 27401928 PMCID: PMC4958297

A Perspective on the Role of Myosins as Mechanosensors.
Greenberg MJ, Arpağ G, Tüzel E, Ostap EM.
Biophysical journal. 2016; 110(12):2568-2576.
PubMed [journal] PMID: 27332116 PMCID: PMC4919425

Structural Analysis of the Myo1c and Neph1 Complex Provides Insight into the Intracellular Movement of Neph1.
Arif E, Sharma P, Solanki A, Mallik L, Rathore YS, Twal WO, Nath SK, Gandhi D, Holzman LB, Ostap EM, Ashish, Nihalani D.
Molecular and cellular biology. 2016; 36(11):1639-54.
PubMed [journal] PMID: 27044863 PMCID: PMC4959310

Force Generation by Membrane-Associated Myosin-I.
Pyrpassopoulos S, Arpağ G, Feeser EA, Shuman H, Tüzel E, Ostap EM.
Scientific reports. 2016; 6:25524.
PubMed [journal] PMID: 27156719 PMCID: PMC4860596

An actin filament population defined by the tropomyosin Tpm3.1 regulates glucose uptake.
Kee AJ, Yang L, Lucas CA, Greenberg MJ, Martel N, Leong GM, Hughes WE, Cooney GJ, James DE, Ostap EM, Han W, Gunning PW, Hardeman EC.
Traffic (Copenhagen, Denmark). 2015; 16(7):691-711. NIHMSID: NIHMS799187
PubMed [journal] PMID: 25783006 PMCID: PMC4945106

Mechanochemical tuning of myosin-I by the N-terminal region.
Greenberg MJ, Lin T, Shuman H, Ostap EM.
Proceedings of the National Academy of Sciences of the United States of America. 2015; 112(26):E3337-44.
PubMed [journal] PMID: 26056287 PMCID: PMC4491760

WHAMM Directs the Arp2/3 Complex to the ER for Autophagosome Biogenesis through an Actin Comet Tail Mechanism.
Kast DJ, Zajac AL, Holzbaur EL, Ostap EM, Dominguez R.
Current biology : CB. 2015; 25(13):1791-7. NIHMSID: NIHMS695769
PubMed [journal] PMID: 26096974 PMCID: PMC4489997

Control of the initiation and termination of kinesin-1-driven transport by myosin-Ic and nonmuscle tropomyosin.
McIntosh BB, Holzbaur EL, Ostap EM.
Current biology : CB. 2015; 25(4):523-9. NIHMSID: NIHMS647751
PubMed [journal] PMID: 25660542 PMCID: PMC4334669