Human Immunoprofiling and Mechanisms

To develop a deeper molecular understanding of checkpoint blockade responses in humans.

Goal A:  Checkpoint blockade cellular and molecular mechanisms       

• Transcriptional profiling, bulk and single cell
• High dimensional flow cytometry and mass cytometry (CyTOF, X50)
• Inhibitory receptor (IR), costimulatory receptor and ligand dynamics during blockade (periphery and tumor micro-environment (TME))
• Transcription factor and transcriptional circuitry
• Metabolism
• Compare Checkpoint to CARs

Goal B:  Human immune profiling developmental project

• Transcriptional profiling, bulk and single cell
• High dimensional flow cytometry and mass cytometry (CyTOF, X50)
• Metabolism
• Imaging Mass Cytometry and other advanced technical approaches

Project Participants: John Wherry, Bob Vonderheide, Roger Cohen, Kate Nathanson, Robert Orlowski, Alex Huang and other researchers