Mouse

Carotid artery or jugular vein (“single line”)
Facilitates either arterial sampling or veinous infusion.

Carotid artery and jugular vein (“double line”)
Facilitates both arterial sampling and veinous infusion.

Portal vein
Facilitates hepatic veinous infusion. 

DSI HD-X11 telemetry probes
Continuous blood pressure, heart rate, EKG, body temperature, and activity at 1 minute recording frequency. 

• For non-invasive tail-cuff blood pressure and heart rate monitoring, use the Kent Scientific CODA BP System. 

DSI HD-XG telemetry probes
Continuous blood glucose, body temperature, and activity at 1 minute recording frequency. 

BodyCAP anipill capsules
Surgically implanted anipill capsule to measure core body temperature measurement through with 1 minute to 1 hour sampling frequency. Continuous core body temperature.

Surgical Development
Please reach out if a new experimental need arises! 

Promethion metabolic cage system
A 16-cage indirect calorimetry system by Sable Systems, that measures VO2, VCO2, RER, energy expenditure, food intake, water intake, and activity levels in a light/dark and temperature-controlled incubator (4 deg C to 30 deg C), with optional running wheels and food access control doors for pair feeding.

13C Stable isotope gas Analyzer
Enables measurement of 13C exhaled in the breath, used concurrently with the Promethion metabolic cage system for stable isotope infusions or bolus treatments.

Metabolic treadmills
2- or 4- lane treadmills to study VO2 max with gas analysis of VO2, VCO2, RER and energy expenditure. 

IKA C3000 Calorimeter
Measures caloric content of a sample, either of a special diet or feces to determine part of the “calories in & calories out” of the energy balance equation.

EchoMRI
Measures fat mass, lean mass, free water, total water.

DEXA PIXImus
Measures fat mass, lean mass, bone mineral content, and bone mineral density.

Stable isotope infusions
Infusion of stable isotopes through the jugular vein and blood sampling from the carotid artery. 

Stable isotope infusions in the Promethion metabolic cage system
Concurrent infusion, blood sampling, and indirect calorimetry gas analysis of VO2, VCO2, RER, energy expenditure, with optional 13C stable isotope gas analysis and ambient temperature options from 4C to 30C. 

Stable isotope infusions in metabolic treadmills
Concurrent infusion, blood sampling, indirect calorimetry gas analysis of VO2, VCO2, RER, energy expenditure, with optional 13C stable isotope gas analysis during treadmill exercise.

FLIR Thermal Camera
Non-invasive thermal imaging of mouse surface temperature (i.e. to assess interscapular BAT temperature). 

Subcutaneous Bio Medic IPTT-300
Injectable probe to measure temperature in specific areas (i.e. interscapular region for BAT temperature).

Glucose clamp (hyperinsulinemic-euglycemic clamp with [3-3H]glucose and 2-[14C]deoxyglucose)
Clamp procedures in the conscious, un-restrained mouse (includes surgery, analysis and plasma insulin measurement). This procedure will allow investigators to assess whole-body insulin action (GIR) and to distinguish between insulin’s effect on endogenous glucose production (EndoRa), glucose utilization (Rd) and to examine insulin’s effect on specific tissues (Rg; muscle, fat, heart, brain). 

Glucose tolerance test
Intraperitoneal or oral delivery of glucose to assess glucose tolerance. 

Insulin tolerance test
Intraperitoneal injection of insulin to assess insulin tolerance. 

Fasting plasma insulin
Plasma collection then insulin ELISA.

Collection of any desired tissue.

Tissue fatty acid uptake is assessed by measuring tissue-specific incorporation of circulating R-2-Bromopalmitic acid [9,10-3H] following intraveous injection which is not metabolized via β-oxidation.

The RMPC works with IDEXX BioAnalytics to measure the analytes listed below in plasma or serum. Please reach out for specific sample collection guidelines if you are interested in this service.

• Liver function panel: ALT, AST 
• Kidney function panel: BUN, CREA
• Custom Chemistry Panel: C-Reactive Protein
• Custom Chemistry Panel:  ALP, ALT, AST, BUN, Bile acids, CREA, GGT, TBIL
• Custom Chemistry Panel: ALT, AST, BUN, CREA
• Custom Chemistry Panel: ALT, AST, TBili
• Other metabolites:  
  • Alanine aminotransferase (ALT; per replicate)
  • Albumin (ALB; per replicate)
  • Alkaline phosphatase (ALP; per replicate)
  • Ammonia (NH3; per replicate)
  • Amylase (AMYL; per replicate)
  • Aspartate aminotransferase (AST; per replicate)
  • Calcium (Ca; per replicate)
  • Cholesterol (CHOL; per replicate)
  • Creatine kinase (CK; per replicate)
  • Creatinine (CREA; per replicate)
  • Glucose (GLUC; per replicate)
  • Inorganic phosphate (PHOS; per replicate)
  • Lactate (LAC; per replicate)
  • Lactate dehydrogenase (LDH; per replicate)
  • Lipase (LIPA; per replicate)
  • Magnesium (Mg; per replicate)
  • Total bilirubin (TBIL; per replicate)
  • Total protein (TP; per replicate)
  • Triglycerides (TRIG; per replicate)
  • Blood Urea Nitrogen (BUN; per replicate)
  • Uric acid (URIC; per replicate)

Intraperitoneal injection of pyruvate to estimate hepatic gluconeogenesis.

The RMPC has 3 mouse housing incubators for any temperature between 4 deg C and 30 deg C.

Intraperitoneal, subcutaneous, retroorbital, and tail vein injections.