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Home » Imaging-based Research » Christopher A. Hunter
Imaging-based Research
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Christopher A. Hunter, Ph.D.
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Professor of Pathobiology in Microbiology
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Department: Microbiology
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Graduate Group Affiliations
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- Cell and Molecular Biology 5c
- Immunology e
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Contact information
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310F Hill Pavilion
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Philadelphia, PA 19104
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3b 380 S. University Avenue
Philadelphia, PA 19104
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Office: 215-573-7772
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Email:
chunter@vet.upenn.edu
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chunter@vet.upenn.edu
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Publications
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Links
eb Search PubMed for articles
9a Hunter Laboratory
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63 Immunology Graduate Group
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eb Search PubMed for articles
9a Hunter Laboratory
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63 Immunology Graduate Group
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Education:
21 9 B.Sc 32 (Zoology, specializing in Parasitology) c
2e University of Glasgow, 1985.
21 a Ph.D. c
2e University of Glasgow, 1989.
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Permanent link21 9 B.Sc 32 (Zoology, specializing in Parasitology) c
2e University of Glasgow, 1985.
21 a Ph.D. c
2e University of Glasgow, 1989.
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Description of Penn IMIG Expertise
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The goal of research in the Hunter laboratory is to better understand how the immune system responds to parasitic infections. Focusing specifically on Toxoplasma gondii, our studies explore host-pathogen interactions at the cellular level. In addition to using genetic approaches to identify specific parasite factors involved in these interactions, we hope to address fundamental questions about which immune cells are important in the initiation of adaptive immunity through the use of transgenic parasites. We are particularly interested in the role of cross-presentation in the development of T cell responses. This compliments our efforts to better define the cytokine networks that regulate the balance between protective and pathological immune responses. Currently the IL-6 family of cytokines (IL-6, 12, 27) are of particular interest to us. Lastly, we are working to better understand the pathogenesis of toxoplasmic encephalitis (TE) in immunocompromised hosts using a variety of imaging techniques to visualize T cell responses in the brain in real time.
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Lab expertise and resources:
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- Immunohistochemistry and multiphoton microscopy to phenotype immune populations
- Routine cytometric based approaches to characterizing lymphocyte function
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IMIG Collaborations:
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- Hunter, Burkhardt and Hammer: analysis of dendritic cell migration through tissues
11 - Hunter and Freedman: calcium flux imaging
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1a4 Wilson, E. H., T. H. Harris, P. Mrass, B. John, E. D. 5. Tait, G. F. Wu, M. Pepper, E. J. Wherry, F. Dzierzinski, D. Roos, P. G. Haydon, T. M. Laufer, W. Weninger, and C. A. Hunter. : Behavior of parasite-specific effector CD8+ T cells in the brain and visualization of a kinesis-associated system of reticular fibers. Immunity 30: 300-311, 2009.
18a Oldenhove, G., Bouladoux, N., Wohlfert, E. A., Hall, J. A., Chou, D., Dos Santos, L., O'Brien, S., Blank, R., Lamb, E., Natarajan, S Kastenmayer, R., Hunter, C., Grigg, M. E., Belkaid, Y: Decrease of Foxp3+ Treg cell number and acquisition of effector cell phenotype during lethal infection. Immunity 31: 772-786, 2009.
138 Jordan, K. A., Wilson, E. H., Tait, E. D., Fox, B. A., Roos, D. S., Bzik, D. J., Dzierszinski, F., and Hunter, C. A. : Kinetics and phenotype of vaccine-induced CD8+ T-cell responses to Toxoplasma gondii. Infect Immun 77: 3894-3901, 2009.
130 John, B., Harris, T., Tait, E., Wilson, E.H., Ng, L., Mrass, P., Dzierszinski, F., Weninger, W., Hunter, C.A: Dynamic imaging of CD8+ T cells and dendritic cells during infection with Toxoplasma gondii. Plos Pathogens 5(7), 2009.
10a Anderson, C.F., Stumhofer, J., Hunter, C.A., and Sacks, D. : IL-27 regulates IL-10 and IL-17 from CD4+ cells in non-healing Leishmania major infection. Journal Immunology 183: 4619-4627, 2008.
12f Kastelein, Robert A. Hunter, Christopher A. Cua, Daniel J.: Discovery and biology of IL-23 and IL-27: related but functionally distinct regulators of inflammation. [Review] [86 refs] Annual Review of Immunology 25: 221-42, 2007.
147 Stumhofer, J.S., J.S. Silver, A. Laurence, P.M. Porrett, T.H. Harris, L.A. Turka, M. Ernst, C.J. Saris, J.J. O'Shea, and C.A. Hunter.: Interleukins 27 and 6 induce STAT3-mediated T cell production of interleukin 10. Nat Immunol Page: 1363-1371, 11 2007.
177 Dzierszinski, F., Pepper, M., La Rosa, D., Wilson, E., Bzik, D., Turka, L., Hunter, CA. and Roos, D.: Antigen presentation through MHC class I occurs via the endogenous pathway in non-hematopoietic cells and professional phagocytes infected with Toxoplasma gondii Infection Immunity 75: 5200-9, 2007.
189 Villarino, Alejandro V. Tato, Cristina M. Stumhofer, Jason S. Yao, Zhengju. Cui, Yongzhi K. Hennighausen, Lothar. O'Shea, John J. Hunter, Christopher A.: Helper T cell IL-2 production is limited by negative feedback and STAT-dependent cytokine signals. Journal of Experimental Medicine 204(1): 65-71, Jan 22 2007.
148 Masek, Katherine S. Fiore, Jim. Leitges, Michael. Yan, Shi-Fang. Freedman, Bruce D. Hunter, Christopher A.: Host cell Ca2+ and protein kinase C regulate innate recognition of Toxoplasma gondii. Journal of Cell Science 119(Pt 21): 4565-73, Nov 1 2006.
240 Stumhofer, Jason S. Laurence, Arian. Wilson, Emma H. Huang, Elaine. Tato, Cristina M. Johnson, Leanne M. Villarino, Alejandro V. Huang, Qiulong. Yoshimura, Akihiko. Sehy, David. Saris, Christiaan J M. O'Shea, John J. Hennighausen, Lothar. Ernst, Matthias. Hunter, Christopher A.: Interleukin 27 negatively regulates the development of interleukin 17-producing T helper cells during chronic inflammation of the central nervous system.[see comment]. Nature Immunology 7(9): 937-45, Sep 2006.
15c Wilson, Emma H. Zaph, Colby. Mohrs, Markus. Welcher, Andy. Siu, Jerry. Artis, David. Hunter, Christopher A.: B7RP-1-ICOS interactions are required for optimal infection-induced expansion of CD4+ Th1 and Th2 responses. Journal of Immunology 177(4): 2365-72, Aug 15 2006.
111 Hirons, Linda. Gardiner, Eleanor J. Hunter, Christopher A. Willett, Peter.: Structural DNA profiles: single sequence queries. Journal of Chemical Information & Modeling 46(2): 743-52, Mar-Apr 2006.
140 Villarino, Alejandro V. Stumhofer, Jason S. Saris, Christiaan J M. Kastelein, Robert A. de Sauvage, Frederic J. Hunter, Christopher A.: IL-27 limits IL-2 production during Th1 differentiation. Journal of Immunology 176(1): 237-47, Jan 1 2006.
19e Shapira, Sagi. Harb, Omar S. Margarit, Juan. Matrajt, Mariana. Han, Jerry. Hoffmann, Alexander. Freedman, Bruce. May, Michael J. Roos, David S. Hunter, Christopher A.: Initiation and termination of NF-kappaB signaling by the intracellular protozoan parasite Toxoplasma gondii. Journal of Cell Science 118(Pt 15): 3501-8, Aug 1 2005.
159 Pepper, Marion. Dzierszinski, Florence. Crawford, Amy. Hunter, Christopher A. Roos, David.: Development of a system to study CD4+-T-cell responses to transgenic ovalbumin-expressing Toxoplasma gondii during toxoplasmosis. Infection & Immunity 72(12): 7240-6, Dec 2004.
173 Villarino, Alejandro. Hibbert, Linda. Lieberman, Linda. Wilson, Emma. Mak, Tak. Yoshida, Hiroki. Kastelein, Robert A. Saris, Christiaan. Hunter, Christopher A.: The IL-27R (WSX-1) is required to suppress T cell hyperactivity during infection.[see comment]. Immunity 19(5): 645-55, Nov 2003.
138 Tato, C.M., G. Martins, F.A. High, K. DiCoco, S.L. Reiner and C.A. Hunter: Cutting Edge: Early production of IFN- by NK cells is independent of epigenetic modification of the IFN- promoter. Journal Immunology Page: 1514-1518, 2003.
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Selected Publications
10d Passos, S. T., Silver, J. S., O’Hara, A., Sehy, D., Stumhofer, J. S., and Hunter, C. A.: IL-6 promotes NK cell production of IL-17 during toxoplasmosis. Journal Immunology 184: 1776-1783, 2010.1a4 Wilson, E. H., T. H. Harris, P. Mrass, B. John, E. D. 5. Tait, G. F. Wu, M. Pepper, E. J. Wherry, F. Dzierzinski, D. Roos, P. G. Haydon, T. M. Laufer, W. Weninger, and C. A. Hunter. : Behavior of parasite-specific effector CD8+ T cells in the brain and visualization of a kinesis-associated system of reticular fibers. Immunity 30: 300-311, 2009.
18a Oldenhove, G., Bouladoux, N., Wohlfert, E. A., Hall, J. A., Chou, D., Dos Santos, L., O'Brien, S., Blank, R., Lamb, E., Natarajan, S Kastenmayer, R., Hunter, C., Grigg, M. E., Belkaid, Y: Decrease of Foxp3+ Treg cell number and acquisition of effector cell phenotype during lethal infection. Immunity 31: 772-786, 2009.
138 Jordan, K. A., Wilson, E. H., Tait, E. D., Fox, B. A., Roos, D. S., Bzik, D. J., Dzierszinski, F., and Hunter, C. A. : Kinetics and phenotype of vaccine-induced CD8+ T-cell responses to Toxoplasma gondii. Infect Immun 77: 3894-3901, 2009.
130 John, B., Harris, T., Tait, E., Wilson, E.H., Ng, L., Mrass, P., Dzierszinski, F., Weninger, W., Hunter, C.A: Dynamic imaging of CD8+ T cells and dendritic cells during infection with Toxoplasma gondii. Plos Pathogens 5(7), 2009.
10a Anderson, C.F., Stumhofer, J., Hunter, C.A., and Sacks, D. : IL-27 regulates IL-10 and IL-17 from CD4+ cells in non-healing Leishmania major infection. Journal Immunology 183: 4619-4627, 2008.
12f Kastelein, Robert A. Hunter, Christopher A. Cua, Daniel J.: Discovery and biology of IL-23 and IL-27: related but functionally distinct regulators of inflammation. [Review] [86 refs] Annual Review of Immunology 25: 221-42, 2007.
147 Stumhofer, J.S., J.S. Silver, A. Laurence, P.M. Porrett, T.H. Harris, L.A. Turka, M. Ernst, C.J. Saris, J.J. O'Shea, and C.A. Hunter.: Interleukins 27 and 6 induce STAT3-mediated T cell production of interleukin 10. Nat Immunol Page: 1363-1371, 11 2007.
177 Dzierszinski, F., Pepper, M., La Rosa, D., Wilson, E., Bzik, D., Turka, L., Hunter, CA. and Roos, D.: Antigen presentation through MHC class I occurs via the endogenous pathway in non-hematopoietic cells and professional phagocytes infected with Toxoplasma gondii Infection Immunity 75: 5200-9, 2007.
189 Villarino, Alejandro V. Tato, Cristina M. Stumhofer, Jason S. Yao, Zhengju. Cui, Yongzhi K. Hennighausen, Lothar. O'Shea, John J. Hunter, Christopher A.: Helper T cell IL-2 production is limited by negative feedback and STAT-dependent cytokine signals. Journal of Experimental Medicine 204(1): 65-71, Jan 22 2007.
148 Masek, Katherine S. Fiore, Jim. Leitges, Michael. Yan, Shi-Fang. Freedman, Bruce D. Hunter, Christopher A.: Host cell Ca2+ and protein kinase C regulate innate recognition of Toxoplasma gondii. Journal of Cell Science 119(Pt 21): 4565-73, Nov 1 2006.
240 Stumhofer, Jason S. Laurence, Arian. Wilson, Emma H. Huang, Elaine. Tato, Cristina M. Johnson, Leanne M. Villarino, Alejandro V. Huang, Qiulong. Yoshimura, Akihiko. Sehy, David. Saris, Christiaan J M. O'Shea, John J. Hennighausen, Lothar. Ernst, Matthias. Hunter, Christopher A.: Interleukin 27 negatively regulates the development of interleukin 17-producing T helper cells during chronic inflammation of the central nervous system.[see comment]. Nature Immunology 7(9): 937-45, Sep 2006.
15c Wilson, Emma H. Zaph, Colby. Mohrs, Markus. Welcher, Andy. Siu, Jerry. Artis, David. Hunter, Christopher A.: B7RP-1-ICOS interactions are required for optimal infection-induced expansion of CD4+ Th1 and Th2 responses. Journal of Immunology 177(4): 2365-72, Aug 15 2006.
111 Hirons, Linda. Gardiner, Eleanor J. Hunter, Christopher A. Willett, Peter.: Structural DNA profiles: single sequence queries. Journal of Chemical Information & Modeling 46(2): 743-52, Mar-Apr 2006.
140 Villarino, Alejandro V. Stumhofer, Jason S. Saris, Christiaan J M. Kastelein, Robert A. de Sauvage, Frederic J. Hunter, Christopher A.: IL-27 limits IL-2 production during Th1 differentiation. Journal of Immunology 176(1): 237-47, Jan 1 2006.
19e Shapira, Sagi. Harb, Omar S. Margarit, Juan. Matrajt, Mariana. Han, Jerry. Hoffmann, Alexander. Freedman, Bruce. May, Michael J. Roos, David S. Hunter, Christopher A.: Initiation and termination of NF-kappaB signaling by the intracellular protozoan parasite Toxoplasma gondii. Journal of Cell Science 118(Pt 15): 3501-8, Aug 1 2005.
159 Pepper, Marion. Dzierszinski, Florence. Crawford, Amy. Hunter, Christopher A. Roos, David.: Development of a system to study CD4+-T-cell responses to transgenic ovalbumin-expressing Toxoplasma gondii during toxoplasmosis. Infection & Immunity 72(12): 7240-6, Dec 2004.
173 Villarino, Alejandro. Hibbert, Linda. Lieberman, Linda. Wilson, Emma. Mak, Tak. Yoshida, Hiroki. Kastelein, Robert A. Saris, Christiaan. Hunter, Christopher A.: The IL-27R (WSX-1) is required to suppress T cell hyperactivity during infection.[see comment]. Immunity 19(5): 645-55, Nov 2003.
138 Tato, C.M., G. Martins, F.A. High, K. DiCoco, S.L. Reiner and C.A. Hunter: Cutting Edge: Early production of IFN- by NK cells is independent of epigenetic modification of the IFN- promoter. Journal Immunology Page: 1514-1518, 2003.
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