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Ana Lia Obaid
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Adjunct Associate Professor of Neuroscience
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Department: Neuroscience
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Contact information
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Department of Neuroscience
3e University of Pennsylvania Perelman School of Medicine
46 B-5 Anatomy-Chemistry Building
Philadelphia, PA 19104-6074
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3e University of Pennsylvania Perelman School of Medicine
46 B-5 Anatomy-Chemistry Building
Philadelphia, PA 19104-6074
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Office: (215) 898-6114
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Email:
obaid@pennmedicine.upenn.edu
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obaid@pennmedicine.upenn.edu
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Publications
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Education:
21 9 M.S. 19 (Biochemistry) c
39 University of Rosario, Argentina, 1969.
21 a Ph.D. 19 (Biochemistry) c
3e University of Buenos Aires, Argentina, 1974.
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21 9 M.S. 19 (Biochemistry) c
39 University of Rosario, Argentina, 1969.
21 a Ph.D. 19 (Biochemistry) c
3e University of Buenos Aires, Argentina, 1974.
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Post-Graduate Training
24 66 Post-doctoral Fellowship, National Research Council (CONICET), Argentina, 1971-1972.
24 7d Research Instructor, Physical Chemistry and Biochemistry, University of Buenos Aires, Argentina, 1972-1975.
24 6a Research Associate Physiology, University of Pennsylvania School of Medicine, 1975-1979.
24 71 Electrophysiological Techniques, Neurobiology, Cold Spring Harbor Laboratory (CSHL), 1979-1979.
24 6d Nervous System of the Leech, Neurobiology, Cold Spring Harbor Laboratory (CSHL), 1979-1979.
24 74 Research Instructor, Physiology and Biophysics, University of Miami School of Medicine, 1980-1981.
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Permanent link24 66 Post-doctoral Fellowship, National Research Council (CONICET), Argentina, 1971-1972.
24 7d Research Instructor, Physical Chemistry and Biochemistry, University of Buenos Aires, Argentina, 1972-1975.
24 6a Research Associate Physiology, University of Pennsylvania School of Medicine, 1975-1979.
24 71 Electrophysiological Techniques, Neurobiology, Cold Spring Harbor Laboratory (CSHL), 1979-1979.
24 6d Nervous System of the Leech, Neurobiology, Cold Spring Harbor Laboratory (CSHL), 1979-1979.
24 74 Research Instructor, Physiology and Biophysics, University of Miami School of Medicine, 1980-1981.
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8c Optical measurements of electrical activity; excitation-secretion coupling; simple nervous systems in vitro; enteric nervous system
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12 KEY WORDS:
7a Voltage-sensitive dyes; light scattering; excitation-secretion coupling; enteric nervous system; submucous plexus
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1b RESEARCH TECHNIQUES
143 Multiple-site optical recording of membrane potential (absorption and fluorescence) and calcium transients with milli-second time resolution; intrinsic optical signals; primary cultures of identified invertebrate neurons; optical recording of electrical activity in mammalian networks, with single cell resolution.
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18 RESEARCH SUMMARY
3c0 I am interested in the application of optical techniques to a variety of physiological problems. These include excitation-secretion coupling, and studies of functional connectivity in small neuronal ensembles. Most of my recent work has employed a system for Multiple Site Optical Recording of Transmembrane Voltage (MSORTV), which, in conjunction with voltage-sensitive dyes, permits one to monitor electrical events in cells or parts of cells not easily accessible with conventional electrophysiological techniques. For example, we have been able to measure the electrical activity (using dyes) and the resultant secretory events (using light scattering) at the nerve terminals of the vertebrate neurohypophysis. We have also used optical techniques to study patterns of electrical activity in simple nervous systems, including small ensembles of identified neurons in tissue culture, and rings of submucosal ganglia of the guinea-pig small intestine.
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Description of Research Expertise
23 RESEARCH INTERESTS8c Optical measurements of electrical activity; excitation-secretion coupling; simple nervous systems in vitro; enteric nervous system
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12 KEY WORDS:
7a Voltage-sensitive dyes; light scattering; excitation-secretion coupling; enteric nervous system; submucous plexus
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1b RESEARCH TECHNIQUES
143 Multiple-site optical recording of membrane potential (absorption and fluorescence) and calcium transients with milli-second time resolution; intrinsic optical signals; primary cultures of identified invertebrate neurons; optical recording of electrical activity in mammalian networks, with single cell resolution.
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18 RESEARCH SUMMARY
3c0 I am interested in the application of optical techniques to a variety of physiological problems. These include excitation-secretion coupling, and studies of functional connectivity in small neuronal ensembles. Most of my recent work has employed a system for Multiple Site Optical Recording of Transmembrane Voltage (MSORTV), which, in conjunction with voltage-sensitive dyes, permits one to monitor electrical events in cells or parts of cells not easily accessible with conventional electrophysiological techniques. For example, we have been able to measure the electrical activity (using dyes) and the resultant secretory events (using light scattering) at the nerve terminals of the vertebrate neurohypophysis. We have also used optical techniques to study patterns of electrical activity in simple nervous systems, including small ensembles of identified neurons in tissue culture, and rings of submucosal ganglia of the guinea-pig small intestine.
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3a Ana Lía Obaid cb B.M. Salzberg: Optical recording of electrical activity in enteric networks using voltage-sensitive dyes. Journal of Visualized Experiments 34: doi: 10.3791/1631, Dec 2009.
16f Fisher, J.A.N., J.R. Barchi, C.G. Welle, G.H. Kim, P. Kosterin, A.L. Obaid, A.G. Yodh, D. Contreras, B.M. Salzberg: Two-photon excitation of potentiometric probes enables optical recording of action potentials from mammalian nerve terminals in situ. J. Neurophysiol. 99(3): 1545-1553, Mar 2008.
1a5 Kim, G.H., P. Kosterin, A.L. Obaid, B.M. Salzberg: A mechanical spike accompanies the action potential in mammalian nerve terminals. Biophys. J. 92: 3122-3129, May 2007 Notes: This article earned a commentary in the New and Notable section of the Biophysical Journal (Eisenberg B. Mechanical spikes from nerve terminals. Biophys J 92: 2983, 2007.).
12a Kosterin, P., G.H. Kim, M. Muschol, A.L. Obaid, and B.M. Salzberg: Changes in FAD and NADH fluorescence in neurosecretory terminals are triggered by calcium entry and by ADP production. J. Membr. Biol. 208: 113-124, Nov 2005.
113 Obaid, A.L., M.E. Nelson, J. Lindstrom, and B.M. Salzberg : Optical studies of nicotinic acetylcholine receptor subtypes in the guinea-pig enteric nervous system. J. Exp. Biol. 208: 2981-3001, Aug 2005.
10f Obaid, A.L., L.M. Loew, J.P. Wuskell, and B.M. Salzberg: Novel naphthylstyryl-pyridinium potentiometric dyes offer advantages for neural network analysis. J. Neurosci. Methods 134: 179-90, Apr 2004.
149 Obaid AL., Koyano T., Lindstrom J., Sakai T., Salzberg BM.: Spatiotemporal patterns of activity in an intact mammalian network with single-cell resolution: optical studies of nicotinic activity in an enteric plexus. J. Neurosci. 19(8): 3073-3093, Apr 1999.
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Selected Publications
138 Kosterin, P., A.L. Obaid & B.M. Salzberg: Long Lasting Intrinsic Optical Changes Observed in the Neurointermediate Lobe of the Mouse Pituitary Reflect Volume Changes in Cells of the Pars Intermedia. Neuroendocrinology 92(3): 158-167, 20103a Ana Lía Obaid cb B.M. Salzberg: Optical recording of electrical activity in enteric networks using voltage-sensitive dyes. Journal of Visualized Experiments 34: doi: 10.3791/1631, Dec 2009.
16f Fisher, J.A.N., J.R. Barchi, C.G. Welle, G.H. Kim, P. Kosterin, A.L. Obaid, A.G. Yodh, D. Contreras, B.M. Salzberg: Two-photon excitation of potentiometric probes enables optical recording of action potentials from mammalian nerve terminals in situ. J. Neurophysiol. 99(3): 1545-1553, Mar 2008.
1a5 Kim, G.H., P. Kosterin, A.L. Obaid, B.M. Salzberg: A mechanical spike accompanies the action potential in mammalian nerve terminals. Biophys. J. 92: 3122-3129, May 2007 Notes: This article earned a commentary in the New and Notable section of the Biophysical Journal (Eisenberg B. Mechanical spikes from nerve terminals. Biophys J 92: 2983, 2007.).
12a Kosterin, P., G.H. Kim, M. Muschol, A.L. Obaid, and B.M. Salzberg: Changes in FAD and NADH fluorescence in neurosecretory terminals are triggered by calcium entry and by ADP production. J. Membr. Biol. 208: 113-124, Nov 2005.
113 Obaid, A.L., M.E. Nelson, J. Lindstrom, and B.M. Salzberg : Optical studies of nicotinic acetylcholine receptor subtypes in the guinea-pig enteric nervous system. J. Exp. Biol. 208: 2981-3001, Aug 2005.
10f Obaid, A.L., L.M. Loew, J.P. Wuskell, and B.M. Salzberg: Novel naphthylstyryl-pyridinium potentiometric dyes offer advantages for neural network analysis. J. Neurosci. Methods 134: 179-90, Apr 2004.
149 Obaid AL., Koyano T., Lindstrom J., Sakai T., Salzberg BM.: Spatiotemporal patterns of activity in an intact mammalian network with single-cell resolution: optical studies of nicotinic activity in an enteric plexus. J. Neurosci. 19(8): 3073-3093, Apr 1999.
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