In vivo insulin signaling (Bolus IV insulin injection + tissue harvesting)
Lipid Metabolism:
In vivo fatty acid uptake assay (R-2-Bromopalmitic acid [9,10-3H])
Energy Homeostasis:
Body composition measurements (NMR or DEXA)
Columbus Instruments Comprehensive Lab Animal Monitoring System (CLAMS): 20 cage thermal cabinet system
Treadmill exercise + metabolic measurements via CLAMS
Sable System Promethion System: 16 cage thermal cabinet system
Stable Isotope Gas Analyzer: Measures the oxidation of both exogenous and endogenous nutrients by allowing simultaneous measurement of stable isotope tracers (13CO2 and C18O2) synchronously with the Promethion data stream
DSI Implantable Telemetry Monitoring:
HD-XG: Continuous measurements of blood glucose, temperature and activity monitoring
HD-X11: Continuous measurements of blood pressure, ECG, heart rate, temperature and activity monitoring
Temperature monitoring:
Cold tolerance test (22°C vs. 4°C) combined with core temperature or BAT temperature measurements
Continuous core body temperature measurements via Anipill capsule peritoneal implants
BAT temperature measurements via subcutaneous implants
FLIR Thermal Imaging (22°C vs. 4°C)
Blood Pressure Measurements:
Non-invasive blood pressure and heart rate (tail cuff method)
Other:
Retro-orbital injection
Grip strength testing
Mouse and Rat Core Services
Lipid Metabolism:
In vivo triglyceride production rate
Energy Homeostasis:
Manual measurement of body weight, food intake and/or supply of special diet/water
IKA C 3000 Bomb Calorimetry
In vivo Metabolic Labeling: Procedure performed in the conscious, un-restrained rodent (includes surgery). Service does not include metabolite measurement by LC-MS
Stable isotope infusions
Costs for individual surgical services: Users are only charged for successful surgeries (animals healthy and functional, patent catheters):
Arterial or jugular catheterization
Arterial and jugular catheterization
Glucose clamp procedures in the conscious, un-restrained rodent (includes surgery, analysis and hormones):
Stable isotope infusions
Hyperinsulinemic-euglycemic clamp
Hyperinsulinemic-euglycemic clamp with [3-3H] glucose
Hyperinsulinemic-euglycemic clamp with [3-3H] glucose and 2-[14C] deoxyglucose
