Laser-scanning Confocal Microscopes

Features:

• Zeiss Axio Observer 7 inverted microscope with Definite Focus 3
• ZEN Blue v. 3.5
• 6 solid-state visible light lasers: 405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 639 nm
• 2 metal alkali PMTs + 32-channel GaAsP detector for fast spectral characterization
• 730 nm laser + two dedicated NIR detectors for imaging dyes such as Cy7, Alexa Fluor 750, etc.
• motorized XY stage + AI sample finder for automated sample location and scanning
• incubation enclosure for live cell imaging
• Objective lenses: 5x/0.16 NA; 10x/0.45 NA; 20x/0.8 NA; 40x/1.1 NA water imm; 63x/1.4 NA oil imm.

Features:

• Zeiss Axio Observer inverted microscope
• ZEN v2.3 software for acquisition
• Excitation laser lines: 405, 458, 488, 514, 561, 633 nm
• Objectives: 10x/0.3 dry, 20x/0.8 dry, 25x/0.8 Imm, 40x/1.2 W, and 63x/1.4 Oil
• Three spectral detection channels: 2 metal alkali PMT + 1 GaAsP detector
• Airyscan module with FAST option for enhanced resolution, speed, and sensitivity
• Environmental chamber and motorized x-y stage for live imaging and tiling applications

Best suited for: confocal imaging of fixed or live samples, including those requiring incubation; FRAP, photoactivation, or photoconversion in live cells or organisms; Airyscan module provides 1.7x increase in resolution; FAST module provides 4x increase in speed.

For new users of this confocal: a handy quick guide to the ZEN software interface.

Features

• Zeiss AxioObserver inverted microscope
• ZEN 2012 software for acquisition
• Excitation laser lines: 405, 458, 488, 514, 561, 594, 633 nm
• Objectives: 10x/0.45 dry; 20x/0.8 dry; 25x/0.8 Imm; 40x/1.1 W; 63x/1.4 Oil
• Two metal alkali PMTs and one 32-channel linear array PMT
• Motorized x-y stage for live imaging and tiling applications

Best suited for: confocal imaging of fixed samples or live samples that do not require incubation; FRAP, photoactivation, or photoconversion in live cells or organisms; FRET; spectral characterization and linear unmixing of unorthodox dye combinations

For new users of this confocal: a handy guide to the ZEN software interface

Features:

• DMi8 inverted microscope
• LAS X software for acquisition
• Extended tunable WLL excitation from 485-790 nm plus fixed 405 nm laser
• Objectives: 10x/0.4 dry; 20x/0.75 dry; 25x/0.95 W; 40x/1.10 W; 40x/1.3 Oil; 63x/1.4 Oil
• Four Power HyD S detectors
• Okolab incubation enclosure
• Motorized x-y stage + Leica's Navigator for tile scans
• FRAP and FRET wizards
• Lightning for deconvolution and TauSense for lifetime-based spectral separation

Best suited for: confocal imaging of fixed or live samples, including those requiring incubation; FRAP, photoactivation, or photoconversion in live cells or organisms; FRET

Features:

• Leica DMI 6000 inverted microscope
• Pulsed white-light laser for tunable excitation from 470 to 670 nm
• CW laser lines for conventional confocal imaging: 405, 458, 488, 514 nm
• Objectives: 10x/0.4 dry; 20x/0.75 Imm; 40x/1.3 Oil; 63x/1.4 Oil; 100x/1.4 Oil
• High-speed resonant scanner for live-cell imaging
• 3 HyD and 2 PMT detectors
• Two depletion lasers (592 and 660 nm) for super-resolution imaging of green and red dyes
• Tokai Hit stagetop incubator
• Motorized xy stage
• LAS X software for acquisition; Huygens Professional software for deconvolution

Features:

• Visible laser lines (all diode or DPSS lasers): 405, 488, 514, 552, 638
• Coherent Chameleon Vision II + MPX wavelength extender for tunable excitation from 740 — 1300 nm
• Leica DM 6000 upright microscope
• Objectives: 10x/0.3 dry; 20x/0.75 Imm; 40x/1.3Oil; 63x/1.4 Oil; 25x/1.0 W motCORR available upon request
• High-speed resonant scanner for live-cell imaging
• 2 HyD and 3 PMT  confocal detectors
• HyD and PMT dual NDDs for reflected light (fluorescence or SHG)
• PMT NDD for transmitted light (SHG)
• Motorized xy stage
• LAS X software for acquisition