Cell and Developmental Biology Microscopy Core

Widefield Microscopes

Non-confocal microscopes suited to imaging thin (< ~25 microns) live or fixed samples

Deltavision Core

Room 1175C BRB II/III

Features

  • Olympus IX70 inverted microscope
  • Photometrics CoolSnap HQ high-resolution CCD camera
  • Applied Precision x-y-z precision motorized stage for multi-point acquisition of image stacks
  • Software-controlled filter wheels with DAPI, FITC, TRITC, Cy5, CFP, filter sets
  • Phase contrast & DIC available
  • Environmental chamber surrounding entire microscope for temperature and CO2 control
  • Deltavision Softworx software for acquisition and processing.

Best suited for: high resolution widefield deconvolution of thin (< 15 microns) fluorescent samples; long time-lapse imaging using transmitted or epifluorescence.

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EVOS FL Auto Imaging System

Room 01-127 Smilow

Features

  • Inverted platform holds a variety of vessels including multi-well plates
  • Dual cameras allow both color and monochrome imaging
  • long-working distance objectives: 4X, 10X, 20X, 40X
  • LED light cubes for DAPI, GFP, RFP, and Cy5
  • environmental chamber with gas mixer for live imaging under normal or hypoxic conditions
  • fully automated stage for tile scanning and multipoint acquisition
  • easy-to-use EVOS software

Best suited for: Single images or tile scans of fixed tissue stained with chromogenic dyes or fixed cells stained with fluorescent markers; live imaging of cells or organisms; hypoxia experiments

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Leica WF - Monochrome Camera

Room 01-126A Smilow

Features:Leica DM6000 Widefield Microscope

  • Leica DM6000 motorized upright microscope
  • Photometrics HQ2 high resolution monochrome CCD camera
  • LAS AF software for acquisition
  • AutoQuant deconvolution package (within LAS)
  • Leica filter sets for DAPI, GFP, Cy3, Texas Red, and Cy5 (spectra here)

Best suited for: high-resolution widefield deconvolution microscopy of thin (<15 microns), fixed samples; widefield epifluorescence imaging of fixed samples.

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Zeiss WF

B30D Anatomy-Chemistry (alcove)

FeaturesZeiss Axioplan Widefield Microscope

  • Zeiss Axioplan upright microscope with Zeiss FluoArc mercury lamp
  • Zeiss AxioCam HRm high-resolution monochrome CCD camera
  • AxioVision 4.5 software for acquisition and analysis
  • Filter sets for standard 4-color imaging (DAPI, FITC, TRITC, Cy5); also CFP and YFP filter set

Best suited for: widefield epifluorescence imaging of fixed, fluorescent samples mounted on slides with coverslips

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