Camera-based Confocals

These systems are optimized for rapid confocal imaging of live or fixed specimens.

Room 1175B BRB II/III

Features

  • Olympus IX71 inverted microscope
  • Visitech VT iSIM scan head for real-time 2x spatial resolution enhancement in x, y, and z
  • Hamamatsu ORCA Quest qCMOS camera
  • Excitation laser lines: 405, 442, 488, 514, 561, 640 nm
  • Gataca Systems iLas2 ablation (355 nm) and FRAP/photoactivation (405 nm) system.
  • Motorized xy stage for multi-point acquisition
  • LCI Chamlide stagetop incubation system for live mammalian cell imaging
  • MetaMorph acquisition software
  • Check out dye compatibility with this system on our FPBase page
  • For users: a cheat sheet showing main MetaMorph software features

Best suited for: fast high-resolution confocal imaging of live or fixed samples

Room 1175D BRB II/III

Features:spinning disk confocal

  • Olympus IX81 inverted microscope
  • Excitation laser lines: 405, 488, 561 (for Hoechst, GFP, RFP)
  • Andor iXon3 EMCCD camera
  • Motorized x-y stage for multi-point acquisition or tile scanning
  • Gataca Systems iLas2 FRAP/photoactivation system (405 nm laser)
  • Okolab stagetop incubation chamber
  • MetaMorph acquisition software
  • Check out dye compatibility with this system on our FPBase page

Best suited for: fast confocal imaging of sensitive, live samples; FRAP, photoactivation, photoconversion in live cells or organisms

For new users of this microscope: a handy 1-page guide to MetaMorph functions

Room 1175D BRB II/III

Crest V3 spinning disk confocalFeatures:

Olympus IX83 inverted microscope

objectives: 10x/0.4, 20/0.8, 40x/0.95 (dry); 60x/1.3 NA (silicone); 100x/1.45 NA (oil)

Excitation laser lines: 405, 445, 488, 520, 555, 630 (check out dye compatibility on our FPBase page)

TWO pco.edge 4.2 bi sCMOS cameras for simultaneous green/red imaging

motorized x-y stage for multi-point acquisition or tile scans

Visitron FRAP/ablation system with 405 nm laser for FRAP/PA and pulsed 355 nm laser for ablation

VisiView software (by Visitron) for acquisition

Okolab incubation enclosure for live cell imaging