Perelman School of Medicine at the University of Pennsylvania

Electron Microscopy Resource Lab

Beckman Foundation
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About Us

The Electron Microscopy Resource Lab is organized into the following:
 

Beckman Center for Cryo-Electron Microscopy

The Beckman Center for Cryo-Electron Microscopy is the part of the Electron Microscopy Resource Laboratory that provides access to state-of the art cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) for structural investigation of macromolecules and cells. The facility houses a Krios G3i microscope equipped with phase plate, K3 Summit Direct Detector camera and Bioquantum GIF energy filter. In addition, the facility has Vitrobot cryo plunger and all accessories needed to perform sample preparation for cryo-EM. The facility is available to Penn research groups and external academic research groups in the greater Philadelphia area.

The Beckman Center for Cryo-Electron Microscopy is reserved for samples that have already been optimized and are ready for extended cryo-EM data collection sessions on the Krios G3i microscope. If your samples are still in the optimization stage, we refer you to our Cryo-EM Screening and Training Facility.

We offer the following services at the Beckman Center for Cryo-Electron Microscopy:

  • High resolution data acquisition of the prescreened Krios-qualified samples on the Krios G3i microscope.
  • Data transfer to the data cluster for internal users or hard drives for external users.
  • The facility is currently restricted to staff for operation and management of the microscope.

Note: The Beckman Center for Cryo-Electron Microscopy does not provide cryo-EM data processing and reconstruction services.

To request services please contact us at: BECKMAN_SERV@lists.upenn.edu

 

 

Darrah Johnson-McDaniel, Ph.D
Technical Director

 

 

 

 

 

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Cryo-EM Training and Screening Lab

The Cryo-EM Screening and Training Facility is the part of the Electron Microscopy Resource Laboratory that provides training and access to state-of the art cryo electron microscopy (cryo-EM) and cryo electron tomography (cryo-ET) for structural investigation of macromolecules and cells. The facility houses two FEI microscopes (T12 and TF20), sample preparation instruments such as Vitrobot as well as two Leica cryo plungers, and all the accessories needed to perform sample screening using negative stain imaging and cryo-EM. The facility is available to Penn research groups and external academic research groups in the greater Philadelphia area.

The goal of The Cryo-EM Screening and Training Facility is to train users to self-sufficiency and proficiency in microscope operation and sample preparation with the ultimate goal of creating independent users who require minimal assistance from staff.

We offer the following services at the Cryo-EM Screening and Training Facility:

  • Training on the two FEI microscopes (T12 and TF20) for negative stain imaging and cryo-EM.
  • Training on sample preparation instruments such as Vitrobot and two Leica cryo plungers.
  • Pay-per-service for negative stain imaging. Note that this service is only available for Penn and CHOP users.

Note: The Cryo-EM Screening and Training Facility does not provide cryo-EM data processing and reconstruction services.

To request services please contact us at SCREEN_SERV@lists.upenn.edu

Sudheer Molugu, Ph.D
Core Director

 

 

 

 

 

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Ultrastructure Core Facility

The Ultrastructure Facility is the part of the Electron Microscopy Resource Laboratory that provides conventional transmission electron microscopy (TEM) of cells and tissues services to Penn research groups and external academic research groups in the greater Philadelphia area. The facility houses a JEOL JEM-1010 microscope, sample preparation instruments and all the accessories needed to perform fixing, processing, sectioning, staining, and imaging.

We offer the following services at the Ultrastructure Facility:

  • Pay-per-service Standard TEM and Immuno TEM for cellular and tissue structure. 
  • Pay-per-service specimen preparation included fixing, processing, sectioning, staining, and imaging.
  • Training on the Reichart Ultramicrotome for independent usage.
  • Training on the JEOL 1010 microscope for independent microscope usage.

Note: The Ultrastructure Facility does not provide data analysis or processing.

To request services please contact us at: ULTRA_SERV@lists.upenn.edu

Dr Biao Zuo

Biao Zuo, M.D, M.S

Technical Director

Inna Martynyuk

Inna Martynyuk, M.S

Research Specialist

 

 

 

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The instruments in the Electron Microscopy Resource Laboratory represent a substantial investment and resource for the University of Pennsylvania research community. To ensure that these resources are available at the optimum performance level and a minimum of downtime, the Internal Cryo-Electron Microscopy Advisory Committee (CEMAC) oversees the services, policies, rules, and rates will be reviewed periodically by the committee and are subject to change.

Internal Cryo-Electron Microscopy Advisory Committee (CEMAC):

· Dr. Vera Moiseenkova-Bell (Faculty Director)

· Dr. Sudheer Molugu

· Dr. Biao Zuo 

· Dr. Darrah Johnson-McDaniel 

· Dr. Kristen Lynch (Chair, Department of Biochemistry and Biophysics)

· Lisa Ward (Financial and Administrative Officer, Department of Biochemistry and Biophysics).