Electron Microscopy Resource Lab

The Beckman Center for Cryo-Electron Microscopy is the part of the Electron Microscopy Resource Laboratory that provides access to state-of the art cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) for structural investigation of macromolecules and cells. The facility houses a Krios G3i microscope equipped with phase plate, K3 Summit Direct Detector camera and Bioquantum GIF energy filter. In addition, the facility has Vitrobot cryo plunger and all accessories needed to perform sample preparation for cryo-EM. The facility is available to Penn research groups and external academic research groups in the greater Philadelphia area.

The Beckman Center for Cryo-Electron Microscopy provides access to screening and optimization of protein samples for cryo-EM data collection on the Krios G3i microscope.

We offer the following services at the Beckman Center for Cryo-Electron Microscopy:

• Screening of cryoEM grids.
• High-resolution data acquisition of prescreened Krios-qualified samples on the Krios G3i microscope.
• Data transfer to the data cluster for internal users or hard drives for external users.
• Note: The Beckman Center for Cryo-Electron Microscopy does not provide cryo-EM data processing and reconstruction services.
• To request services please contact us at: BECKMAN_SERV@lists.upenn.edu

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The TEM Training Facility provides training and access to Tecnai T12 microscope for learning basic operation of TEM as well as optimizing samples with negative stain EM. The facility houses a FEI T12 microscope and cryo-EM sample preparation instruments such as Vitrobot and Leica cryo plungers. We offer the following services at the TEM Training Facility:

• Training on FEI T12 Microscope for negative stain imaging.
• Training on sample preparation instruments such as Vitrobot and two Leica cryo plungers.
• Pay-per-service for negative stain imaging.

To request services please contact us at SCREEN_SERV@lists.upenn.edu

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The Ultrastructure Facility is the part of the Electron Microscopy Resource Laboratory that provides conventional transmission electron microscopy (TEM) of cells and tissues services to Penn research groups and external academic research groups in the greater Philadelphia area. The facility houses a JEOL JEM-1010 microscope, sample preparation instruments and all the accessories needed to perform fixing, processing, sectioning, staining, and imaging.

We offer the following services at the Ultrastructure Facility:

• Pay-per-service Standard TEM and Immuno TEM for cellular and tissue structure. 
• Pay-per-service specimen preparation included fixing, processing, sectioning, staining, and imaging.
• Training on the Reichart Ultramicrotome for independent usage.
• Training on the JEOL 1010 microscope for independent microscope usage.

Note: The Ultrastructure Facility does not provide data analysis or processing.

To request services please contact us at: ULTRA_SERV@lists.upenn.edu

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