Figure 1: Features of Alveolar Type 2 Cells AT2 cells
(A) Human AT2 cells in culture stained for lipid using Nile Red to identify lamellar bodies. “N” = nuclei.
(B) Transmission electron micrograph of human alveolar type 2 cell from normal adult lung showing lamellar bodies (LB®) containing a dense core granule, multivesicular bodies (MVB), and microvilli (“mv”).
(C) Immunohistochemistry of human AT2 cell monolayer stained for proSP-C (Red) and DAPI (nuclei).
(D) Western blotting of isolated mouse AT2 for pro-SP-C showing the 21 kD proSP-C primary translation product (arrow) and low molecular weight processed intermediates (bracket)
(E) Western blotting of preparations of human AT2 cells prepared using SP-B antisera. The proSP-B primary translation product (thick arrow), 25 kD intermediates (thin arrow) and mature SP-B (bracket) are identified. In addition dimeric mature SP-B (arrowhead) is present.