groove

Penn Center for AIDS Research

Susan R. Weiss

faculty photo
Professor of Microbiology
Associate Dean for Postdocotral Research Training, University of Pennsylvania
Director, Office of Biomedical Postdocotoral Programs, University of Pennsylvania
Department: Microbiology

Contact information
203A Johnson Pavilion
3610 Hamilton Walk
Philadelphia, PA 19104-6076
Office: (215) 898-8013
Fax: (215) 573-4858
Lab: 215-898-3551
Graduate Group Affiliations
Education:
B.A. (Biology)
Brandeis University, 1971.
Ph.D. (Microbiology and Molecular Genetics)
Harvard University, 1975.
Post-Graduate Training
Predoctoral Fellow. Laboratory of Dr. Michael A. Bratt, Ph.D. Thesis work: Studies on the messenger RNA and poly(A) of Newcastle Disease Virus, Harvard University School of Medicine, 1971-1975.
Postdoctoral Fellow. Laboratory of Dr. J. Michael Bishop, Area of Research: Purification and characterization of the messenger RNAs of avian sarcoma-leukosis viruses, Department of Microbiology and Immunology, University of California, San Francisco., 1976-1980.
Sabbatical with Dr. Robert Fletterick, project: Expression and structural studies of the papain like proteinase of murine coronavirus, strain A59, Department of Biochemistry, University of California, San Francisco, 1994-1995.
Permanent link
 
> Perelman School of Medicine   > Faculty   > Details

Description of Research Expertise

Research Interests
- Murine coronavirus pathogenesis, central nervous system, liver and lung
- Murine coronavirus antagonism of the OAS-RNase L pathway
- Organ specific virus- host interactions
- Viral and cellular phosphodiesterases
- Middle Eas Respiratory Syndrome Coronavirus pathogenesis
- Role of inflammasome related cytokines in murine coronavirus acute disease and chronic demyelination


Key words: murine coronavirus, human respiratory coronavirus, viral pathogenesis, interferon antagonist.

Description of Research

Susan Weiss, Ph.D.

Professor of Microbiology

Office Address:
University of Pennsylvania School of Medicine
203 A Johnson Pavilion
3610 Hamilton Walk
Philadelphia, PA 19104-6076

TEL 215-898-8013
LAB 215-898-3551
FAX 215-573-4858
weisssr@mail.med.upenn.edu


RESEARCH SUMMARY

My lab studies coronavirus pathogenesis. We use murine coronavirus, mouse hepatitis virus (MHV) infection of mice as a model system for the study of: 1) acute viral encephalitis; 2) chronic demyelinating diseases such as Multiple Sclerosis and 3) virus-induced hepatitis. We have the important tools of a well-developed animal model system and two reverse genetic systems with which to manipulate the viral genome. Human coronaviruses are primarily respiratory viruses, and include the common cold viruses OC43 and 229E as well as the emerging viruses MERS and SARs that cause severe and life threatening diseases. We are beginning to study human coronavirus interactions with respiratory tract cells. Our long-term goal is to elucidate the viral and cellular determinants of coronavirus tropism and pathogenesis in the brain, the liver and the lung. Much of our current work focuses on coronavirus-encoded antagonists of type I interferon, specifically virus-encoded phosphodiesterase that antagonize the OAS-RNase L pathway. Another direction in our lab is the study of the role of inflammasome related cytokines in viral clearance as well as both acute and chronic MHV induced disease.


Currently we are carrying out the following studies:

1. Virus-encoded phosphodiesterases (PDE), that act as type I interferon antagonists. The MHV ns2 gene encodes a phosphodiesterase activity that specifically cleaves 2-5A, the activator of Ribonuclease (RNase) L, a potent antiviral response induced by type I interferon and infection. Homologous proteins with RNase L antagonist activates are also expressed by some other coronaviruses, toroviruses, other members of the coronavirus superfamily as well as type A rotaviruses, that cause severe gastrointestinal infections of children. Activation of RNase is cell type specific and most most robust in myeloid cells and antagonism of RNase L is required for the induction of hepatitis by MHV. We are currently investigating the mechanisms underlying activation of this pathway and antagonism by virus induced phosphodiesterase and their role in pathogenesis.

2. Human coronaviruses and RNase L antagonism. A group of other group 2a Betacorovaviruses encode ns2 homologs that we have recently found to have phosphodiesterase activity. We are characterizing these PDEs now and beginning to investigate a possible role for RNase L antagonism in the respiratory tract, the major target of most human coronaviruses including the lethal, emerging pathogen SARS and MERS.

2. Inflammasome associated cytokines and MHV acute and chronic disease pathogenesis. We are investigating the roles of the two inflammasome related cytokines IL-1 an IL-18 in coronavirus pathogenesis. This includes understanding their roles in protection against and contribution to acute encephalitis and hepatitis and in addition a possible role in the chronic demyelinating disease.

3. Role of receptor in MHV entry, spread and pathogenesis. The only known receptor for MHV is the carcinoembryonic antigen protein, CEACAM 1. However, the highly neurovirulent MHV-JHM strain spreads cell to cell in the absence of the expression of this receptor. Using transgenic CEACAM 1 deficient mice (ceacam 1a -/-), we have found that JHM can replicate and cause CNS disease and mortality in the absence of viral receptor. We are using a selection of MHV mutants and tropism variants in combination with primary cells derived from wild type and ceacam 1a-/- mice to elucidate the mechanism of viral neuron to neuron spread and the role of receptor independent spread in disease. We also plan to discover alternate receptors used by MHV in the absence of ceacam1a.


Rotation Projects

1. Coronavirus RNase L antagonists: Investigation of the mechanism by which MHV ns2 as well as human coronavirus ns2 homologs inhibit the oligoadenylate synthetase (OAS)-ribonuclease (RNase) L interferon stimulated anti-viral pathway. This includes understanding the contributions of host OAS and RNase L gene expression, host interferon response as well as virus encoded phosphodiesterase in the activation and antagonism of RNase L. Projects also include investigation of activation of the OAS-RNase L pathway in myeloid cells versus other cell types and how this impacts on pathogenesis. These projects involve studies of virus in primary cell types as well as in vivo experiments in mice and involve carrying out a wide variety of techniques, including viral pathogenesis, cell culture, cloning, in vitro protein expression and real time qPCR.

2. Mechanisms of MHV neurovirulence. Investigation of the roles of IL-1 and IL-18 in MHV pathogenesis and clearance (comparing effects in the brain and liver) as well as virus induced demyelination. This includes comparison of viral replication and clearance during infection of IL-1R and IL-18R knockout mice with C57/Bl6 wild type mice and the mechanisms underlying difference between pathgoenesis of wild type an knockout mice. This project includes cell culture, infections of mice, isolation of immune cells, flow cytometry as well as Western blots.




Lab personnel:

Dillon Birdwell- Graduate Student
Ruth Elliott - Research Specialist
Stephen Goldstein- Graduate Student
Yize (Henry) Li- Postdoctoral Researcher
Judith Phillips- Research Associate
Yuyan Wang, Visiting Scholar

Selected Publications

Phillips Judith M, Gallagher Tom, Weiss Susan R: Neurovirulent Murine Coronavirus JHM.SD Uses Cellular Zinc Metalloproteases for Virus Entry and Cell-Cell Fusion. Journal of virology 91(8), Apr 2017.

Li Yize, Banerjee Shuvojit, Goldstein Stephen A, Dong Beihua, Gaughan Christina, Rath Sneha, Donovan Jesse, Korennykh Alexei, Silverman Robert H, Weiss Susan R: Ribonuclease L mediates the cell-lethal phenotype of double-stranded RNA editing enzyme ADAR1 deficiency in a human cell line. eLife 6, Mar 2017.

Goldstein Stephen A, Thornbrough Joshua M, Zhang Rong, Jha Babal K, Li Yize, Elliott Ruth, Quiroz-Figueroa Katherine, Chen Annie I, Silverman Robert H, Weiss Susan R: Lineage A Betacoronavirus NS2 Proteins and the Homologous Torovirus Berne pp1a Carboxy-Terminal Domain Are Phosphodiesterases That Antagonize Activation of RNase L. Journal of virology 91(5), Mar 2017.

Kindler Eveline, Gil-Cruz Cristina, Spanier Julia, Li Yize, Wilhelm Jochen, Rabouw Huib H, Züst Roland, Hwang Mihyun, V'kovski Philip, Stalder Hanspeter, Marti Sabrina, Habjan Matthias, Cervantes-Barragan Luisa, Elliot Ruth, Karl Nadja, Gaughan Christina, van Kuppeveld Frank J M, Silverman Robert H, Keller Markus, Ludewig Burkhard, Bergmann Cornelia C, Ziebuhr John, Weiss Susan R, Kalinke Ulrich, Thiel Volker: Early endonuclease-mediated evasion of RNA sensing ensures efficient coronavirus replication. PLoS pathogens 13(2): e1006195, Feb 2017.

Li Yize, Weiss Susan R: Antagonism of RNase L Is Required for Murine Coronavirus Replication in Kupffer Cells and Liver Sinusoidal Endothelial Cells but Not in Hepatocytes. Journal of virology 90(21): 9826-9832, Nov 2016.

McCarthy James B, Weiss Shira R, Segovich Kristin T, Barbot Baptiste: Impact of psychotic symptoms on cognitive functioning in child and adolescent psychiatric inpatients with severe mood disorders. Psychiatry research 244: 223-8, Oct 2016.

Bradley William P, Boyer Mark A, Nguyen Hieu T, Birdwell L Dillon, Yu Janet, Ribeiro Juliana M, Weiss Susan R, Zamboni Dario S, Roy Craig R, Shin Sunny: Primary Role for Toll-Like Receptor-Driven Tumor Necrosis Factor Rather than Cytosolic Immune Detection in Restricting Coxiella burnetii Phase II Replication within Mouse Macrophages. Infection and immunity 84(4): 998-1015, Apr 2016.

Sui Baokun, Huang Junhua, Jha Babal K, Yin Ping, Zhou Ming, Fu Zhen F, Silverman Robert H, Weiss Susan R, Peng Guiqing, Zhao Ling: Crystal structure of the mouse hepatitis virus ns2 phosphodiesterase domain that antagonizes RNase L activation. The Journal of general virology 97(4): 880-6, Apr 2016.

Thornbrough Joshua M, Jha Babal K, Yount Boyd, Goldstein Stephen A, Li Yize, Elliott Ruth, Sims Amy C, Baric Ralph S, Silverman Robert H, Weiss Susan R: Middle East Respiratory Syndrome Coronavirus NS4b Protein Inhibits Host RNase L Activation. mBio 7(2): e00258, Mar 2016.

Li Yize, Banerjee Shuvojit, Wang Yuyan, Goldstein Stephen A, Dong Beihua, Gaughan Christina, Silverman Robert H, Weiss Susan R: Activation of RNase L is dependent on OAS3 expression during infection with diverse human viruses. Proceedings of the National Academy of Sciences of the United States of America 113(8): 2241-6, Feb 2016.

back to top
Last updated: 09/21/2017
The Trustees of the University of Pennsylvania