Participating Cores
Please note the event will be split into morning (10-12:15) and afternoon sessions (12:45-3), with participating cores changing over during lunch (12:15-12:45).
Please click here for the event map.
Animal Model Core New Bolton Center - RRID:SCR_022435 (Penn Vet)
The Institute for Medical Translation New Bolton Center is an ecosystem for multidisciplinary collaboration and the mentoring of graduate and undergraduate students in veterinary surgery and the translational sciences. The central theme of our activities focuses on “Musculoskeletal Tissue Injury and Repair”. This theme is broad as 1) it includes all musculoskeletal tissue types, such as bone, cartilage, disc, ligament, meniscus, muscle, and tendon, 2) it is also focused as it takes advantage of commonalities in approaches across tissue types and 3) it is clinically significant as it fosters development of assays, procedures and knowledge in preclinical animal and human models of translational relevance. This “multidisciplinary ecosystem” and has emerged as a recognized home for translational research using large animal models across the Penn campus as a technical and intellectual resource for the broader Philadelphia community and beyond. Studies start as small proof of concept studies progressing all the way to IND/IDE enabling studies (GLP) in support of regulatory submissions or we help develop and submit grants with translational relevance on collaborative submissions to federal funding agencies (NSF, NIH).
Thomas Schaer, VMD
Director
tpschaer@vet.upenn.edu
Table Number: 11
Live Core Facility Presentation: 11:50-11:55
Bioinformatics Core (Wistar)
The Bioinformatics Shared Resource continuously develops new and efficient approaches to data analysis as a response to emerging research needs. Facility functions include: statistical analyses and computational modeling for all types of high-throughput data; advanced bioinformatics tools for integrative cancer biology; and data management. Routine data analyses include large scale information datasets (omics data) generated by high-throughput technologies, which address the following areas:
• Gene expression (RNA-seq, single cell RNA-see, smRNA-seq, Nanostring)
• Gene regulation (ChIP-seq, Cut & Run, ATAC-seq, epigenetic profiling, Hi-ChIP, HiC)
• Genome and transcriptome sequencing (alternate splicing, RNA editing, gene fusion, SNP and INDEL mutation detection, CNV)
• Biomarkers (discovering markers in mRNA ,miRNA and protein expression data)
• Proteomic analyses (mass spectrometry-based spectra, LCMS, DIGE, RPPA, etc.)
• Pathway and network analysis
• Integration of multi-platform data
• Other customized data analysis projects
Andrew Kossenkov, PhD
Managing Director
akossenkov@wistar.org
Table Number: 1 & 2
Bioinformatics Core Facility (BIC) - RRID:SCR_022374 (PSOM)
The Bioinformatics Core (BIC) of the Institute for Biomedical Informatics (IBI) provides professional bioinformatics services that include data analysis and consultation to the University of Pennsylvania Biomedical research community. The BIC is dedicated to analyze various Next-Generation Sequencing (NGS) data, generated locally within NGSC and also elsewhere.
Taehyong Kim, PhD
Director
taehyong@pennmedicine.upenn.edu
Table Number: 20
Biomedical Research Support (Wistar)
The Biomedical Research Support Core (BRSC) provides a robust infrastructure to support mechanistic, patient-oriented research. The BRSC manages the resource commitment associated with clinical studies, including supporting compliance with regulatory directives governing research in Human Subjects. Services include phlebotomy and blood product procurement, tissue microarrays, collection of pathological specimens and support for clinical studies. This includes data collection, storage and extraction, data quality control, site monitoring, regulatory reporting, and connection with statistical teams for data analysis.
Livio Azzoni, PhD, MD
Managing Director
azzoni@wistar.org
Table Number: 1 & 2
BioRepository Resource Center - BioRC (CHOP)
The Biorepository Resource Center (BioRC) improves the breadth and access to bio-banked specimens and information resources across the CHOP community of investigators. With a capacity for approximately 2 million to 3 million samples, the facility serves CHOP’s biobanking needs, avoiding specimen duplication, preserving precious materials, and providing organized data and materials. The BioRC operates under the following principles: An Operational Committee, which coordinates central and investigator-specific biobanking resources and programs at CHOP, governs and approves requests to utilize the BioRC; BioRC facilitates integration and enhancement of access to information about biorepository specimens and resources; BioRC assists investigators in developing new projects that require the collection and processing of shareable samples not currently available, or helps investigators with existing projects who would like to migrate storage and management of their shareable banked specimens and data to the BioRC. BioRC institutes and follows best-practice standard procedures for collection, processing, and storage of samples to ensure high quality specimens and data for all CHOP investigators.
Paula Oliver, PhD
Associate Professor, Department of Pathology
Head, Division of Protective Immunity
University of Pennsylvania
Children's Hospital of Philadelphia
paulao@pennmedicine.upenn.edu
David Stokes, PhD
Director
stokesdg@chop.edu
Carrie Coleman-Campbell,
Project/Program Manager
colemanc@chop.edu
Susan Jones
Lab Manager
joness23@chop.edu
Michael Kelly
IT Supervisor
KellyM11@chop.edu
Rich Tustin
Lab Manager
tustinr@chop.edu
Core Facility Email: BioRC@chop.edu
Table Number: 4
Biostatistics & Data Management Core - BDMC (CHOP)
The BDMC supports investigators from all pediatric medicine subspecialties who are pursuing studies ranging from small, narrowly defined basic science projects to large, multi-site clinical trials. We provide valuable, upfront, statistical study design and comprehensive planning assistance to guide investigator’s research process from the beginning to the end. The Biostatistics team provides consultative and analytic biostatistical expertise to enhance the design and quality of basic, pre-clinical, and clinical studies. The Biostatistics team uses effective statistical methods that pertain to investigators’ specific study aims and facilitate accurate interpretation and communication of results. The Data Management team works collaboratively with investigators to meet their unique project data management needs during critical phases of the research process. We apply our comprehensive knowledge and the latest technology tools to generate accurate, complete, reliable data to be used for further analysis.
Linda Sprouse
Project Administrator
sprousel@chop.edu
lindasprouse@westat.com
Julie Pappas
Co-director, Data Management
pappas@chop.edu
JuliePappas@westat.com
Table Number: 21
CAG Biorepository Core (CHOP)
Established in 2006, the CAG Biorepository is one of the world’s largest and most diverse pediatric biobanks, hosting more than 600,000 biological samples that have been genotyped or sequenced in CAG’s CLIA and CAP-accredited high-throughput centers. This core offers services in DNA and RNA extraction.
James Snyder
Laboratory Manager
snyder3@chop.edu
Table Number: 6
CAG Genotyping Core (CHOP)
Established in 2006, the CAG Genotyping Laboratory is fully equipped with state-of-art liquid handling technology and experienced staff members. The CAG genotyping core offers both standard and custom array project design. With the capacity to process >2300 samples/week and generate >480 million genotypes per day, the genotyping core fully integrates with our Biorepository and Bioinformatics group, offering the collaborator the opportunity for a seamless experience from DNA extraction to data release.
Maria Lemma
Laboratory Manager
garrism@chop.edu
Table Number: 6
CAG Sequencing Core (CHOP)
Established in 2012, the CAG Sequencing Laboratory is fully equipped with state-of-art technology and experienced staff members. Sequencing operations are integrated with our Biorepository and Bioinformatics teams to keep all stages of a collaborator’s studies connected. The CAG sequencing lab offers cutting edge technologies in the Genomics field: Novaseq, Single Cell 10xGenomics, Bionano, Fluidigm confirmation, Sanger Sequencing (including fragment analysis).
Jonathan Billings
Laboratory Manager
billingsj@chop.edu
Table Number: 6
CDB Microscopy Core Facility - RRID:SCR_022373 (PSOM)
The CDB Microscopy Core at the Perelman School of Medicine provides researchers from Penn, CHOP, and the surrounding area with access to 13 light microscopes, a scanning electron microscope, and image analysis workstations.
Andrea Stout, PhD
Director
astout@pennmedicine.upenn.edu
Jasmine Zhao
Light Microscopy Specialist
xinyu2@pennmedicine.upenn.edu
Yuri Veklich
SEM Specialist
yuri@pennmedicine.upenn.edu
Table Number: 28
Cell & Animal Radiation Core Facility (CARC) - RRID:SCR_022377 (PSOM)
The Cell and Animal Radiation Core (CARC) is one of the most advanced cell and animal radiation research facilities in the US, from providing basic cell and animal radiation needs, to modeling state-of-the-art radiation modalities currently used in the clinic. The CARC provides “turn-key” services to users for performing precision, image-guided radiotherapy with both Photons and Protons (the latter including FLASH radiotherapy) on cells, explant tissues, rodent models and canine patients. A dedicated team of Physicists also provides expert dosimetry and treatment planning capabilities. The CARC instrumentation consists of two Image Guided SARRP 200 Small Animal Radiation Research Platforms (Xstrahl), a research proton beamline (IBA, Roberts Proton Center), X-RAD 320ix cabinet x-ray irradiator (Precision X-Ray) and two Cs gamma-ray irradiators (Shepherd Mark I).
Shiva Shahrampour, PhD
Technical Director
shiva.shahrampour@pennmedicine.upenn.edu
Table Number: 10
Live Core Facility Presentation: 10:30-10:35
Cell Center Services - RRID:SCR_022391 (PSOM)
The Cell Center Services Facility provides basic cell culture training and services in various cell culture and related procedures e.g. Mycoplasma and Endotoxin testing. The services include cell culture at various scales, expansion of primary cells, seed cell cultures from on-site cell bank, cell storage; large scale growth of hybridoma and other cell lines followed by antibody purification by protein G column or recombinant protein production; EBV induced transformation of lymphocytes. It offers hybridoma generation by cell fusion and screening and, the transfection of mammalian cells. The facility prepares specialized cell culture media, Drosophila media and, various tissue culture and molecular biological reagents.
Sabine Baxter
Technical Manager
baxters@pennmedicine.upenn.edu
Table Number: 33
Cell Center Stockroom - RRID:SCR_022399 (PSOM)
The Cell Center Stockroom serves University of Pennsylvania investigators and affiliate institutions by coordinating relations with various suppliers of molecular biological research materials. This involves not only bulk purchasing of these products, but the negotiation of discounts and convenient delivery arrangements. There are over 1,100 products on-site for immediate delivery in the Stockroom. Special ordering of non-regularly stocked products is available from 28 bioreagent vendors with discounted pricing and sometimes overnight delivery.
Joshua Varrone
Assistant Manager
jvarrone@pennmedicine.upenn.edu
Table Number: 33
Center for Human Phenomic Science (CHPS) RRID:SCR_022402 (PSOM)
The goal of the CHPS is to provide the resources, environment, operations, and training to support and promote high-quality clinical and translational research by qualified investigators.
Carl Shaw, Med, MBE
shawcarl@pennmedicine.upenn.edu
Tracey Caputo, ML, CPA
tracey.caputo@pennmedicine.upenn.edu;
Jessica Lenzo, PHP, BSN, RN, OCN
jessica.lenzo@pennmedicine.upenn.edu
Table Number: 8
Center for Human Phenomic Science - CHPS (CHOP)
The Center for Human Phenomic Science (CHPS) provides the resources, environment, operations, and training to promote high-quality clinical and translational research by qualified investigators. CHPS was formed with the receipt of the Clinical and Translational Science Award (CTSA), an NIH Roadmap initiative. CHPS is a CHOP-Penn collaboration with child- and adult-specific facilities, as well as joint components, supporting research at the interface of basic and clinical research with a focus on the development of new and safer therapeutic entities.
John Krall, LCSW
Administrative Director
krallj@chop.edu
Eileen Ford, MS, RDN, FAND
Administrative Manager
forde@chop.edu
Beth Schwartzman, RD, LDN, CDE
Clinical Research Project Manager
schwartzmann@chop.edu
Table Number: 9
Chemical & Nanoparticle Synthesis Core (CNSC) - RRID:SCR_022390 (PSOM)
The CNSC supports researchers by providing in-house expertise in medicinal chemistry, metal chelate chemistry, polymer synthesis, nanoparticle production and surface functionalization, and site-specific antibody labeling. Our chemists provide custom, problem-specific support for researchers in medical, chemical, and materials science fields. Specific services include: consultation and assistance with the design of chemical/medicinal compounds, in-house synthesis of chemical/medicinal compounds, custom nanoparticle design and synthesis, custom nanoparticle surface functionalization, polymer and dendrimer synthesis, and site-specific antibody labeling.
Andrew Tsourkas, PhD
Director
atsourk@seas.upenn.edu
Jeffrey Winkler, PhD
Merriam Professor of Chemistry
Winkler@sas.upenn.edu
Davit Jishkariani, PhD
Associate Technical Director
davit.jishkariani@pennmedicine.upenn.edu
Core Facility Email: CNSC@pennmedicine.upenn.edu
Table Number: 17
Live Core Facility Presentation: 2:30-2:35
CHOP Microbiome Center (CHOP)
Looking for help with sequencing and analytics to advance your project? The CHOP Microbiome Center is the sequencing and analytical resource of the PennCHOP Microbiome Program, providing end-to-end solutions for microbiome research.
Kyle Bittinger, PhD
Analytics Core Director
bittingerk@chop.edu
Ahmed Moustafa, BPharm, PhD
Sequencing Core Director
moustafaam@chop.edu
Ruth Fahey, PhD
faheyr@chop.edu
Administrative Manager
Mistura Faro
Resource Coordinator II
farom@chop.edu
Table Number: 19
Live Presentation: 2:00-2:05
CHOP Research Institute Research Information Services (CHOP)
The Research Information Services (RIS) Department consists of several teams providing expert advice alongside innovative and effective technology-based solutions to Children's Hospital of Philadelphia's Research Institute.
Nick Kight
RIS Outreach Manager
kightn@chop.edu
Table Number: 20
Live Presentation: 1:10-1:15
CHOP Transgenic Core (CHOP)
The Transgenic Core is a service sponsored by the CHOP Research Institution to enable investigators to drive cutting edge basic and bench-to-bedside research. The Mission of the Core is to provide a cost-effective fast method for the generation and preservation of genetically altered mice for the research community. The Core facility, under the direction of Adele Harman, provides a large number of services to the research community including: CRISPR/Cas9 RNA microinjection, CRISPR/AAV Transduction (new service), CRISPR/Cas9 RNP electroporation, transgenic mouse production, Embryonic Stem Cell (ES) microinjection into blastocysts, pathogen-free strain rederivation, recovery of cryopreserved strains, embryo and sperm cryopreservation and rescue of precious genetically unique strains. The state-of-the-art facility maintains specialized equipment necessary to perform the above operations. The facility is located in the barrier unit of the Colket Translational Research Building (CTRB).
Adele Harman
Technical Director
harmana@chop.edu
Table Number: 6
CHOP-Penn Proteomics Core Facility (CHOP/PSOM)
The CHOP-PENN Proteomics Core Facility provides a variety of state-of-the-art services for investigators at CHOP, University of Pennsylvania, and outside institutions. We have implemented a strict quality control procedures and optimized bottom-up mass spectrometry (MS) protocols for a variety of quantitative proteomics projects. Our core has a proven track record in omics-scale analysis of whole proteome and enriched sub-proteomes. The core is equipped with three state-of-the-art mass spectrometers. A Bruker timsTOF Pro2 coupled with a NanoElute UPLC and Evosep One, a Thermo Exploris 480 and a Thermo QEHF coupled with Ultimate 3000 nano UPLCs. We have access to a variety of commercial and open-source software to offer advanced statistical analysis including data processing and visualization, network and pathway analysis, and identification of statistically significant protein changes. We are able to tailor sensitive and specific methods for protein detection and quantification according to your needs. The Core has extensive experience and expertise with LC-MS based bottom up proteomics for analysis of post translational modifications, and will provide end to end service and collaboration throughout all the phases of the project from planning and experimental design through data interpretation.
Lynn Spruce
Technical Director
spruce@chop.edu
Table Number: 30
Live Core Facility Presentation: 10:10-10:15
Clinical In Vivo Gene Therapy Core (CHOP)
Led by experts with more than 10 years of gene therapy clinical trial experience, the CIGT team aims to offer more breakthrough therapies to our patients sooner, by streamlining the startup of complex gene therapy trials and ensuring licensed gene therapies are available in our clinics.
Julianne LaRosa, MPH
Clinical Research Coordinator
larosaj1@chop.edu
Table Number: 31
Clinical Research Computing Unit (CRCU) - RRID:SCR_022404 (PSOM)
The CRCU serves as a "Core Research Facility" within the University to allow researchers and investigators access to its services across a wide range of research programs and medical disciplines. These core services allow the CRCU to participate in various research programs within the University, as well as with external research-related entities. The CRCU provides a complete set of services to facilitate the collection, storage and management of clinical research data for any size research study conducted by University investigators and their collaborators. Whether the CRCU performs the myriad duties of a Data Coordinating Center for multi-center research networks or creates a simple database to organize cohort data, each project receives the right amount of specialized expertise and attention to ensure successful completion with accurate and reliable research data. The CRCU has extensive experience providing design, development and project and technology support services to the entire Penn Medicine clinical and translational research enterprise.
Lisa Wesby, MS
Associate Director
Project Operations & Compliance (POCO)
wesby@pennmedicine.upenn.edu
Florence Speiser, MBA
Senior Manager
Research Design, Development, & Quality Assurance (RDQA)
fspeiser@pennmedicine.upenn.edu
Core Facility Email: CRCU-Contact@lists.upenn.edu
Table Number: 22
Clinical Vector Core (CHOP)
The Raymond G. Perelman Center for Cellular and Molecular Therapeutics (CCMT) Clinical Vector Core is a non-profit facility that provides state-of-the-art current Good Manufacturing Practices (cGMP) clinical vector manufacturing of adeno-associated virus (AAV) and lentiviral (LV) vectors.
The Core provides platform manufacturing for LV and AAV vectors. Products for clinical use are manufactured in compliance with FDA cGMP regulations for Phase 1 and 2 clinical trials. The core also offers GLP-grade products to support pre-clinical pharmacology and toxicology studies, and research-grade pilot batches for proof-of-principle studies. Finally, the core offers testing services for long-term stability and device compatibility studies and provides Chemistry, Manufacturing and Control support for Investigational New Drug (IND) and Investigational Medicinal Product Dossier (IMPD) applications.
The Children’s Hospital of Philadelphia Clinical Vector Core is an approved supplier of investigational products for the National Institutes of Health (2021) and welcomes national and international projects from academia, industry, and government.
Johannes van der Loo, PhD
Director
vanderlooj@chop.edu
Table Number: 25
Comparative Medicine Services Core -CMSC (CHOP)
Comparative Medicine Services core currently supports pre-clinical studies, to generate data for future GLP studies and submission to FDA. We are working on GLP-compliance to support IND-enabling studies.
Melanie McFadden, DVM, MLAS, LATG
Technical Director
mcfaddenmc@chop.edu
Amy Muehlmatt, MLAS, LATG
Operations Manager
muehlmatta@chop.edu
Table Number: 12
Live Core Facility Presentation: 11:10-11:15
Cooperative Human Tissue Network - CHTN - RRID:SCR_022407 (PSOM)
The CHTN is a prospective human tissue procurement service within the Department of Pathology at HUP that assists investigators with collection, processing and preservation of research samples. Our staff will personalize sample acquisition and processing to meet project requirements. Samples (malignant, normal, diseased, etc.) and biofluids can be preserved in a range of methods (fresh, frozen, fixed, etc.).
Dee McGarvey, QBRS
Director, CHTN Eastern Division
dfitzsim@pennmedicine.upenn.edu
Table Number: 7
CRISPR/Cas9 Mouse Targeting Core Facility - RRID:SCR_022378 (PSOM)
The CRISPR-Cas9 mouse targeting core at the Perelman School of Medicine, University of Pennsylvania, has been operational since February 2017. Jorge HENAO-MEJIA has served as Scientific Director and Leonel JOANNAS as the Technical Director. The core is in the Institute for Immunology (IFI) and the Scientific and Technical Directors are part of the IFI. In addition, this recently established core is composed of one research technologist “A” who works under our guidance. The mission of the CRISPR-Cas9 targeting core is to streamline procedures to facilitate the use of the CRISPR/Cas9 genome editing technology by the larger UPenn/CHOP community to generate novel mouse genetic tools rapidly and economically. This core has had a significant positive impact on the community. Researchers who work with mice and rats to model human diseases have enthusiastically taken up these tools. Projects that may have taken years using older techniques, such as examining the effects of disrupting several genes at once, generating point mutations, or inserting fluorescent proteins, now take just a couple of months. It is also easier to produce more subtle changes, like tweaking just a few DNA nucleotides in a gene in a way that simulates a variation found in humans. In addition, our R&D efforts in this area should enable UPenn/CHOP to remain at the forefront of this technology. By using the CRISPR/Cas9 technology, we can now generate pre-clinical mouse and rat models for research purposes in a matter of 3 to 4 weeks. Moreover, it allows us to generate models that more closely mimic human diseases. Hence, this truly has been a revolution in the biomedical research field and its impact is already observed in the clinics. We have already developed more than two hundred different targeting strategies during our first two and a half years of being operational. The CRISPR-Cas9 Mouse Targeting Core at Penn is a state-of-the-art facility that has an outstanding record of accomplishment in the production of genetically altered mice and rats for investigators at Penn and outside institutions, within and the state of Pennsylvania, as well as overseas.
Jorge Henao-Mejia, PhD
Scientific Director
jhena@pennmedicine.upenn.edu
Leonel Joannas, PhD
Technical Director
ljoannas@pennmedicine.upenn.edu
Table Number: 4
Live Core Facility Presentation: 11-11:05
CyTOF Service Center, Institute for Immunology and Immune Health - RRID:SCR_022410 (PSOM)
The CyTOF Helios is the mass cytometer available to the Penn and VA community. The technology uses Time-Of-Flight mass spectrometry of rare isotopes as a readout. Practically, in contrast to flow cytometry, the use of metal isotope-labeled antibodies instead of fluorescent–labeled antibodies, allows the separation of a large number of isotope-labelled reagents (currently, more than 40 different isotope labels are available). This technology enables multi-parametric high-dimensional single cell analysis of >40 markers per cell, with minimal background and compensation issues.
The CyTOF Core offers a wide variety of CyTOF-related services including reagent distribution, consultation, antibody-conjugation, data acquisition and data analysis. Please contact the Technical Director for details.
Takuya Ohtani, PhD
Technical Director
takuya@pennmedicine.upenn.edu
Table Number: 16
Electron Microscopy Resource Laboratory - RRID:SCR_022375 (PSOM)
The EMRL at the Perelman School of Medicine, University of Pennsylvania, is a training and service facility dedicated to providing both conventional transmission electron microscopy (TEM) of cells and tissues and state of the art cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) for structural investigation of macromolecules and cells.
Sudheer Molugu, PhD
Director
Electron Microscopy Resource Laboratory
sudheer.molugu@pennmedicine.upenn.edu
Stefan Steimle, PhD
Director
Beckman Center for Cryo-Electron Microscopy
Stefan.Steimle@Pennmedicine.upenn.edu
Table Number: 27
Live Core Facility Presentation: 10:50-10:55
Engineered mRNA & Targeted Nanomedicine Core Facility - RRID:SCR_023665 (PSOM)
The Engineered mRNA and targeted nanomedicine core is designed to address the infrastructural barrier to clinical and translational research in gene therapy, vaccine development (cancer and infectious disease), stem cell reprogramming, and other non-viral gene therapy-based applications by providing high-quality in vitro transcribed messenger RNA (IVT-mRNA) and lipid nanoparticles (LNPs). The Engineered mRNA and targeted nanomedicine core support the ITMAT and PSOM faculty, ITMAT partner institutions, and members of the Center for Targeted Therapeutics and Translational Nanomedicine (CT3N).
Mohamad-Gabriel Alameh, PhD
Core Co-Leader
mg.alameh@pennmedicine.upenn.edu
Garima Dwivedi, PhD
Core Manager
garima.dwivedi@pennmedicine.upenn.edu
Benjamin Davis, PhD
Research Specialist
benjamin.davis@pennmedicine.upenn.edu
Lily Lo
Research Specialist
lily.lo@pennmedicine.upenn.edu
Table Number: 32
Extracellular Vesicle Core Facility - RRID:SCR_022444 (Penn Vet)
The Extracellular Vesicle (EV) Core Facility located in the Rosenthal building at 3800 Spruce Street provides comprehensive or selected services in the necessary isolation, quantification and characterization of EVs. Isolation of EV is based on size exclusion using high-performance (SEC- HPLC) or gravity fed (e.g. iZon column) liquid chromatography, ultracentrifugation, and/or density gradient ultracentrifugation. We can accurately characterize EV particle size distribution and concentration using resistive pulse sensing techniques (nCS1, Spectradyne, LLC). Immunophenotype can be accomplished using nanoscale flow cytometry and/or chip array (ExoViewTM) techniques. We additionally provide services in training and education for individuals and lab groups in all methods above and study design consultation to ensure that your EV work is of the highest quality and prepared for high impact publication in this exciting and rapidly growing field.
Luca Musante, PhD
Director
musante@upenn.edu
Table Number: 18
Live Core Facility Presentation: 1:50-1:55
Flow Cytometry Core (CHOP)
The Flow Cytometry Core Laboratory provides access to state of the art instrumentation and professional flow cytometry services to members of the research community at Children's Hospital of Philadelphia (CHOP) and University of Pennsylvania. The lab is located in Suite 1207 on the 12th floor of the Leonard and Madlyn Abramson Pediatric Research Center at CHOP. The facility is equipped with 8 analyzers of various types (Aurora, LSR, CytoFLEX, Accuri) and six cell sorters (Aurora CS, FACSAria, FACSJazz, FACSMelody, MoFlo Astrios, BioSorter). Our staff has extensive expertise with cell sorting and analytical flow cytometry applications, including sample processing for surface and intracellular staining, functional assays, complex multi-color analyses, etc.
Florin Tuluc, MD, PhD
Director
tuluc@chop.edu
Jennifer Murray
Senior Technical Specialist
murrayjb@chop.edu
Table Number: 14
Live Core Facility Presentation: 2:50-2:55
Flow Cytometry Core (Wistar)
The Flow Cytometry Shared Resource provides investigators with the technological resources and professional assistance for high quality, multiparameter flow cytometry analyses and sorting. The Facility is capable of cell sorting (sterile, at speeds up to 30,000 cells/sec) from homogeneous or mixed cell populations based on up to 48 fluorochromes, sorting up to six separate populations simultaneously, including human-derived samples at BSL-2 level. Facility personnel aid investigators in creating efficient and cost-effective experimental designs, through optimizing cytometry-specific reagent and fluorochrome selection, and offer assistance in operation of analysis instruments. Technical support is also provided for analyses of flow and imaging cytometry data for publication, presentation, and inclusion in grant applications, management of cytometric data (storage, archiving, and retrieval), and management of a site license for low-cost post-acquisition analysis software.
Jeffrey Faust, BA, MBA
Managing Director
jfaust@wistar.org
Table Number: 1 & 2
Genomics Core (Wistar)
The Genomics Shared Resource serves as a hub for consultation and scientific interaction relating to nucleic acid-based methods. It provides expertise and support to ensure the best possible outcomes for genomic-related projects. The Facility supports several state-of-the-art platforms for a wide variety of nucleic acid-based studies, including massively parallel sequencing and routine capillary sequencing. The Facility also supports projects related to RNA sequencing, ChIP-Seq, smRNA-Seq, whole genome, exome and targeted resequencing, bulk TCR and BCR immune repertoire profiling, single cell sequencing, and gene expression studies using Quant-seq or the NanoString platform. In addition, we support SNP genotyping analysis using TaqMan assays, KIR allele genotyping, HLA typing and microsatellite assays. Consultation is provided to assist with experimental design and for the development of custom services.
Sonali Majumdar, MS
Managing Director
smajumdar@wistar.org
Table Number: 1 & 2
High Throughput Sequencing Core (CHOP)
The High Throughput Sequencing Core provides next generation sequencing on Illumina® platforms now and Nanopore sequencing in the future. HTS Core provides bulk library preparation and/or sequencing for a broad range of projects as well as consultation on experimental design of sequencing projects.
Teodora Orendovici
Technical Director
orendovict@chop.edu
Table Number: 17
High-Throughput Screening Core Facility - RRID:SCR_022379 (PSOM)
The High-throughput Screening Core (HTSC) in the Perelman School of Medicine provides expertise and infrastructure to facilitate the development and implementation of professional High-throughput screening services. We have the capabilities to perform HTS in both biochemical and cell-based assays using state-of-the-art equipment. We can perform genetic or small molecule screens to identify genes or small molecule modulators of diverse processes in diverse models including primary cells. The HTSC educates and assists scientists with HTS assay development, optimization, miniaturization, implementation, analysis, and validation. We can develop new assays or run validated HTS assays. In addition, the HTSC maintains libraries of siRNA, shRNA, cDNA, and FDA-approved/FDA-like organic small molecule libraries for HTS. Moreover, we routinely prepare custom libraries for single-point or dose-response screening. The HTSC provides robotics infrastructure and technically trained staff for primary HTSs as well as secondary screens to validate candidates. The HTSC also provides assistance with the preparation of grant applications and Letters of Support, offering HTS resources and analysis expertise for the proposed research.
Sara Cherry, PhD
Scientific Director
cherrys@pennmedicine.upenn.edu
David Schultz, PhD
Technical Director
dschultz@pennmedicine.upenn.edu
Tori Gordillo
Administrative Coordinator
tori.gordillo@pennmedicine.upenn.edu
Table Number: 18
Histology Core (Wistar)
The Histotechnology Shared Resource provides services for fixing, processing and paraffin or OCT-embedding of all types of tissues for light microscopy (e.g. routine stains, immunohistochemistry or in situ hybridization). The Facility staff performs routine hematoxylin, eosin staining, immunochemistry and FISH staining, as well as specialized staining and slide preparation for immunohistochemistry and in situ hybridization. Frozen sectioning is also available, including consultation regarding freezing and fixing techniques to optimize experimental results.
Fangping Chen, HT (ASCP)
Managing Director
fchen@wistar.org
Table Number: 1 & 2
Human Immunology Core - RRID:SCR_022380 (PSOM)
The Human Immunology Core (HIC) offers translational research support primarily for investigators in the Perelman School of Medicine. The cell product service offers purified human cell subsets from HLA-typed apheresis donors, collected under a blanket IRB protocol and distributed to dozens of labs 2-3 times per week. Clinical trial support services include blood and biopsy processing, viable cell cryopreservation and storage, and sample shipping. The HIC also offers turnkey cellular immunology assays such as conventional and digital ELISA, ELISPOT, and flow cytometry. The HIC BCR/TCR sequencing services use next-gen technology for bulk DNA, RNA, or single-cell human BCR/TCR sequencing, murine BCR sequencing, and include basic data analysis using standard or project-specific approaches. Principal Investigators and trainees alike use the HIC’s scientific consultation services for assistance with matters ranging from assay design to quotations or letters of support for extramural funding applications.
Nina Luning Prak, MD, PhD
Director
luning@pennmedicine.upenn.edu
Honghong Sun, PhD
Technical Director
hsun2@pennmedicine.upenn.edu
Table Number: 10
Live Core Facility Presentation: 2:40-2:45
Human Pluripotent Stem Cell Core (CHOP)
The Human Pluripotent Stem Cell (HPSC) Core, established in 2008, is part of the Raymond G. Perelman Center for Cellular and Molecular Therapeutics. An infrastructure and solid foundation has been created for the generation, differentiation, and gene editing of induced pluripotent stem cell (iPSC) lines. Using the CRISPR-CAS9 gene editing technology, isogenic control and disease-specific iPSC lines can be created for modeling human disease to study mechanism, development, and establish new therapeutic modalities. The Core provides enrichment training courses for 1) growth and maintenance of iPSC lines, 2) differentiation to mesoderm, endoderm or ectoderm, and 3) gene editing.
Deborah French, PhD
Director
frenchd@chop.edu
Jean Ann Maguire, PhD
Technical Director
MaguireJ@chop.edu
Elisa Waxman, PhD
Scientist
waxmane@chop.edu
Alyssa Gagne, BS
Research Technician
gagnea@chop.edu
Table Number: 33
Imaging Core (Wistar)
The imaging of complex cellular structures is used to determine how the temporal and spatial organization of regulatory events within cells, tissues and organisms impacts both normal and pathological processes. The state-of-the-art Imaging Shared Resource provides access to standard and advanced optical imaging systems capable of reaching these goals and offers assistance with advanced image analysis solutions. Researchers may be trained for unassisted use of all core instrumentation, while full-service assistance by facility staff is also available for quantitative or qualitative image capture. The Facility also offers expert technical assistance with experimental design to optimize imaging results, enabling users to get more out of the imaging technology.
James Hayden, BA, RBP, FBCA
Managing Director
jhayden@wistar.org
Table Number: 1 & 2
Induced Pluripotent Stem Cell Core Facility - RRID:SCR_022426 (PSOM)
The iPSC core at UPenn provides services including derivation of iPS cells from somatic cells, Hands-on training to researchers in ES/iPS cell culture; differentiation of iPS cells into many lineages. The core serves as a resource for sharing iPS cell lines and iPSC technology within the UPenn and the broader scientific community.
Wenli Yang, PhD
Director
wenliyan@pennmedicine.upenn.edu
Jingli Cai, PhD
Scientific Manager
jingli.cai@pennmedicine.upenn.edu
Table Number: 32
Information Services Advisory Center - ISAAC (PSOM)
IS-concierge service that provide personalized support to faculty and staff in the Penn Medicine community on IS-related questions on Penn applications, research, IS security, and more.
Jennifer Moody
Senior Manager
jennifer.moody@pennmedicine.upenn.edu
Table Number: 19
Institute for Biomedical Informatics Clinical Research Informatics Core - RRID:SCR_022409 (PSOM)
The Institute for Biomedical Informatics (IBI) Clinical Research Informatics Core (CIC) provides data brokering and analytics services of clinical data for clinical and translational research in which the goal is to learn actionable healthcare knowledge and develop impactful solutions for improving patient care. We specialize in data extraction, integration, standardization, and analytics of clinical data from the electronic health record (EHR) as well as research databases including PennG&P, Penn COVID-19 i2b2 database, and the IQVIA Medical Record Database using artificial intelligence, natural language processing (NLP), ontologies, machine learning, and visual analytics. We also provide translation of research products into healthcare operations through coordination of projects with Penn Medicine Corporate Information Services, Penn Data Analytics Center (DAC), Penn Data and Analytics (PennDnA) and the Center for Applied Health Informatics (CAHI). The IBI CIC is a member of the Penn-sponsored Honest Broker program.
Danielle Mowery, PhD, MS, MS, FAMIA
Scientific Director
dlmowery@pennmedicine.upenn.edu
Heather Williams
Technical Director
hwilli@pennmedicine.upenn.edu
Table Number: 24
Live Core Facility Presentation: 1:30-1:35
Johnson Foundation Biophysical & Structural Biology Core Facility - RRID:SCR_022414
The Department of Biochemistry and Biophysics at the University of Pennsylvania is privileged to have a wide range of unique equipment and expertise to facilitate modern biophysical characterization and structural analysis of proteins and other biomolecules. These facilities, funded in large part through the generosity of the Johnson Foundation, are available for use by the research community at Penn and beyond.
Kushol Gupta, PhD
Director
kgupta@pennmedicine.upenn.edu
Table Number: 26
Live Core Facility Presentation: 2:10-2:15
Large Animal Imaging Core - LAIF (CHOP)
The LAIF core is dedicated to bridging the gap between basic science and clinical applications by focusing on preclinical MRI, a vital resource in the field. This facility provides researchers with access to a state-of-the-art 3.0T MRI scanner, specifically designed to support large animal models. Our goal is to enable researchers to translate their findings from basic science to the direct benefit of patients. By utilizing the LAIF core, researchers gain the ability to test and validate hypotheses derived from clinical research in animal models, thereby gaining invaluable insights into various diseases and treatment approaches. One major advantage of preclinical MRI is the ability to utilize the same imaging methods and pulse sequences in both preclinical and clinical scanners, offering a unique opportunity for seamless translation from bench to bedside. Moreover, the LAIF core goes beyond providing access to advanced imaging technology. We offer a range of additional services to support researchers in their projects. These include free consultations on experimental design, optimization of imaging sequences for clinical translation, grant development focused on imaging, training, and education on MR safe practices, as well as analyzing imaging data. The LAIF core facilitates advanced MR image-guided interventional and non-invasive procedures to study various disease areas, such as neurodegenerative, cardiothoracic, and reproductive diseases. Our imaging applications cover a wide range of techniques, including anatomical, diffusion, perfusion, spectroscopy, angiography, tissue elastography, functional imaging, flow quantification, and susceptibility weighted imaging (SWI). We are pleased to inform you that the LAIF core is open to all investigators at CHOP, as well as external investigators from academic and commercial backgrounds who wish to utilize our MRI instrument. To ensure the highest standards in animal-based research, we work closely with the Division of Veterinary Resources (DVR)team, who provide valuable support and expertise. We strongly encourage investigators to consult with them regarding any scientific, medical, and compliance issues related to animal-based research, and to obtain IACUC approval before utilizing core services. To streamline project submissions and instrument scheduling, we utilize the iLab platform. You can find additional information, including pricing, standard operating procedures (SOPs), and access to the LAIF core, on our website and the internal iLab portal. For further inquiries regarding core services and its operations, please do not hesitate to contact us at CHOP_LAIF_Core@chop.edu.
Pavan Jella
jellap@chop.edu
Vincent Derose
derosev@chop.edu
Table Number: 9
Metabolomics Core Facility - RRID:SCR_022381 (PSOM)
The Penn Metabolomics Core provides expertise in untargeted and targeted metabolomics of biological samples using liquid chromatography/mass spectrometry. Our mission is to perform the assays and assist in the interpretation of the results. The core is available to the entire Penn research community, external research investigators, and industry.
Chris Petucci, PhD
Director
christopher.petucci@pennmedicine.upenn.edu
Dina Abbasian, BS
Research Specialist
dina.abbasian@pennmedicine.upenn.edu
Clarissa Shoffler, BS
Research Specialist
clarissa.shoffler@pennmedicine.upenn.edu
Table Number: 29
Molecular Pathology & Imaging Core Facility (MPIC) - RRID:SCR_022420 (PSOM)
The Molecular Pathology and Imaging Core is a resource for researchers at Penn, CHOP, and beyond. We offer histological services such as processing, embedding, sectioning, and staining paraffin fixed and frozen samples including difficult samples such as organoids, spheroids, and cell pellets. MPIC also has resources and equipment to assist with immunofluorescent and immunohistochemical staining and imaging as well as expertise and equipment for spatial biology such as Visium and RNAscope.
Kate Bennett
Technical Director
bennk@pennmedicine.upenn.edu
Table Number: 11
Molecular Screening/Protein Expression Facility (Wistar)
The Molecular Screening and Protein Expression Facility provides biochemical and cell-based assay development expertise to establish assays suitable for high-throughput screening and compound profiling. In addition, we offer protein expression services in insect cells. Other hosts such as bacteria and mammalian cell lines as well as protein purification are offered on a case by case basis. In addition, the facility manages glycerol stocks the TRCN shRNA library and individual clones are available upon request.
Joseph Salvino, PhD
Scientific Director
jsalvino@wistar.org
Joel Cassel
Managing Director
jcassel@wistar.org
Table Number: 1 & 2
Neurobehavior Testing Core Facility- RRID:SCR_022386
The Neurobehavior Testing Core provides expertise, facilities and training for a variety of procedures that span multiple behavior domains. Behavior output related to sensory, motor, social, communication, affect, learning and memory are routinely assessed. An initial consultation with core staff will be helpful in designing a battery of procedures tailored to address the aims of each specific project. Core personnel can perform the entire study or after training, personnel from the PI may perform procedures at a reduced cost. The Neurobehavior Testing Core is subsidized by ITMAT and the IDDRC at CHOP/UPenn to reduce direct costs to the investigator.
W. Timothy O'Brien Ph.D.
obrienw@pennmedicine.upenn.edu
Director, Neurobehavior Testing Core
The University of Pennsylvania, Perelman School of Medicine
10-166 Smilow Center for Translational Research
3400 Civic Center Bldg 421
Philadelphia, PA 19104
Phone: (215) 898-0476
Table Number: 34
OCRC Tumor BioTrust Collection - RRID:SCR_022387 (PSOM)
The OCRC Tumor BioTrust Collection collects cancer tissue specimens from all cases of gynecologic cancers. We handle collection, processing, storage, and distribution of primary and recurrent tumor samples. All samples collected have clinical annotation including demographic patient profiles, pathological & clinical notations, treatment history, and detail disease information.
Euihye Jung, MBA
Technical Director
euihye@pennmedicine.upenn.edu
Core Facility Email: TBCrequests@pennmedicine.upenn.edu
Table Number: 5
Live Core Facility Presentation: 1-1:05
Office of Clinical Research (PSOM)
The Office of Clinical Research seeks to promote human research for the advancement of healthcare while ensuring the highest level of research participant safety and facilitating the highest quality research by realizing the best research standards through adherence to university and government research policies and regulations; supporting investigators and research teams through process improvement, innovative technologies, and education and training initiatives; propagating best operational practices to maximize the efficiencies of research activities; collaborating with University organizations involved with human research.
OCR can provide guidance and expertise with regulatory submissions and exemptions, contract support with external sponsors, research budgets and finance, training, research data management and systems, monitoring, clinicaltrials.gov and inspection support.
Lorri Schieri, MBA
Chief Operating Officer
Clinical and Translational Science
schieril@upenn.edu
Nevine Mikhail
Administrative Coordinator
nevinem@upenn.edu
Table Number: 21
Office of Environmental Health & Radiation Safety (Penn)
The Office of Environmental Health and Radiation Safety (EHRS) promotes health, safety and environmental protection in teaching, research, health care and administrative activities by providing services, advice, and compliance resources. Come learn more about how we can assist you throughout your research career at Penn!
Elizabeth Bassett, PhD
Assistant Biosafety Officer
littauer@ehrs.upenn.edu
Table Number: 23
Live Presentation: 10:20-10:25
Pancreatic Islet Cell Biology Core Facility - RRID:SCR_022411
The Pancreatic Islet Cell Biology Core offers guidance and support for physiological and morphometric assessment of pancreatic islet function and growth. We will discuss and help to plan your experimental design and can provide services including rodent islet isolation. We offer or train labs in an expansive range of assays including islet hormone secretion, which can be assessed in static “batch” incubations or by more informative perifusions. The core also has the advanced technology and expertise to perform individual islet and single cell fluorescence imaging (Cai 2+), respirometry with islet batches using a Seahorse Extracellular Flux Analyzer along with the BioTek Cytation 5 for Seahorse normalization, perifusion coupled with respirometry for our investigators.
Nicolai Doliba, PhD, DSc
Technical Director
nicolai@pennmedicine.upenn.edu
Andrea Rozo, PhD
Assistant Technical Director
arozo@pennmedicine.upenn.edu
Table Number: 34
Pathology Core Laboratory (CHOP)
The Pathology Core provides histopathology, Immunohistochemistry, tissue microarray, and imaging services to researchers at Children’s Hospital of Philadelphia Research Institute and the surrounding academic community. Our technical and professional staff has extensive experience in all aspects of tissue processing, histological techniques, antibody work-up, and morphologic analysis. In addition, we offer tools to facilitate high throughput analysis of tissue sections and imaging.
Daniel Martinez
Director
martinezd@chop.edu
Table Number: 12
Penn Cytomics & Cell Sorting Shared Resource Laboratory - RRID:SCR_022376 (PSOM)
Penn Cytomics is the sole flow cytometry shared resource laboratory (SRL) at the University of Pennsylvania. Our facility has 35+ instruments, which include analyzers, cell sorters, and small particle detectors. We also have a dual-fluorescence cell counter/viability instrument and a tissue dissociator for cell preparation. We provide both on-site and off-site support to our instrument users, including analyzer and cell sorter training. We also have a research and development team that collaborates/consults with principal investigators in developing high-dimensional panels, as well as staining, acquisition, and analysis. Penn Cytomics is an Abramson Cancer Center supported facility, and has been recognized as "exceptional" by the National Cancer Center for over 30 years. Recently, our core became a "Recognized SRL" through the International Society for Advancement of Cytometry.
Derek Jones, PhD, SCYM(ASCP)CM
Senior Technical Director
jonesder@pennmedicine.upenn.edu
William Murphy, BA, SCYM(ASCP)CM
Technical Director
william.murphy@pennmedicine.upenn.edu
Table Number: 15
Penn Genomics & Sequencing Core Facility - RRID:SCR_022383 / RRID:SCR_022389 / RRID:SCR_022382 (PSOM)
The Penn Genomics and Sequencing Core is the only University-wide facility that provides comprehensive genomics and sequencing services on multiple platforms. The core offers Next-Generation-Sequencing (NGS) using Illumina MiSeq, NextSeq 2000 and NovaSeq 6000, and Sanger sequencing on ABI capillary sequencer. The NGS services include consultation, experimental design, nucleic acid extraction, library preparation and quality control, and sequencing followed by data processing and data analysis. For single- cell genomics, PGSC uses 10x Genomics Single-Cell Controller and has been doing single-cell experiments for over five years. The Sanger capillary sequencer enables microsatellite-based genotyping, MLPA and other applications of fragment analysis, and cell line authentication. Whole genome and targeted molecular profiling are performed on multiple platforms. The Core supports quantitative RNA and DNA profiling on Affymetrix GeneChips, Fluidigm BioMark HD, and ABI QS12 Flex real-time PCR instrument. The molecular biological services include PCR-based mouse genotyping, cloning, subcloning, and site-directed mutagenesis.
Tapan Ganguly, PhD
Core Director
gangulyt@pennmedicine.upenn.edu
Jonathan Schug, MSE, PhD
Technical Director
Next Generation Sequencing Facility
Hetty Rodriguez, MS
Molecular Profiling Facility
hettymr@pennmedicine.upenn.edu
Jennifer-Marie Rosado
Research Technician
jmrosado@pennmedicine.upenn.edu
Erik Toorens
Laboratory Manager
toorens@pennmedicine.upenn.edu
Table Numbers: 27 & 28
Live Core Facility Presentation: 2:20-2:25
Penn Gnotobiotic Mouse Facility (PGMF) - RRID:SCR_022384
Gnotobiotic mice (mice harboring a defined microbial community) are a critical tool to uncover the causal relationship between the microbiome and disease, and to determine the mechanisms through which commensal microbes influence the host. To facilitate microbiome research at Penn, The Gnotobiotic Mouse Facility, which is part of the PennCHOP Microbiome Program, manages a colony of germ-free mice and offers support with any experimentation involving the microbiome. Germ-free mice can be used to study the effects of specific bacteria on a phenotype of interest, to determine the impact of microbial colonization at different body sites, and to study how different environmental interventions, such as different infections, diets or drugs, modulate the microbiome and thereby affect the host. Thus, germ-free mice can be used, in the fields of microbiology and immunology, metabolism, neuroscience, cancer, development, toxicology, and as a powerful tool for drug discovery. The Penn Gnotobiotic Mouse Facility offers several different experimental platforms, including a positive and negative pressure isocage system as a solution for short-term studies (ranging from days to several weeks), where experimentation with sterile mice can be performed at large scale using multiple different experimental conditions. Germ-free mice are transferred from isolators to isocage racks for experimentation, where they are kept under fully gnotobiotic conditions. This facilitates mono- and poly-colonization experiments as well as experiments with advanced instrumentation for behavioral experiments or imaging modalities under germ-free conditions. In addition, the Core maintains several common strains of germ-free mice and mice at different ages that are available upon request and provides re-derivation services for generating customized germ-free and gnotobiotic mouse strains.
Ken Cadwell, PhD
Core Director
Maayan Levy, PhD
Director
Christoph Thaiss, PhD
Director
Dmytro Kobuley
Technical Director
Core Facility Contact Email: penngf@pennmedicine.upenn.edu
Table Number: 35
Penn Libraries Holman Biotech Commons/Research Data & Digital Scholarship
Holman Biotech Commons: The Holman Biotech Commons is Penn’s biomedical library and recently completed a major renovation of the main floor. Librarians serving Penn Nursing, PSOM, Biology, and Penn Medicine are based here and are available for research consultations, library orientations, and workshops on the library’s various databases and resources. Additionally, Holman is home to the Bollinger Digital Fabrication Lab, providing research poster printing service as well as 3D printing and laser cutting services. Holman offers 20 group study rooms, a collaborative classroom, and a reading room. Spaces still in development include: One Button Recording Studio, Design Thinking Studio, and Mixed Reality Lab. The library is open to all – visit our website for our hours, to reserve a room, and to reach out to any of our staff.
Research Data & Digital Scholarship: The Penn Libraries Research Data & Digital Scholarship team facilitates data-driven and data-literate research and scholarship across the disciplines and fosters informed and ethical data communities at Penn.
https://www.library.upenn.edu/holman
https://www.library.upenn.edu/page/bollinger-digital-fabrication-lab https://www.library.upenn.edu/page/research-data-digital-scholarship
Hannah Rutledge, BA, MLIS, PhD
Director
Biotech Commons
mhr@upenn.edu
Manuel de la Cruz Gutierrez, MLS, PhD
Director of Data & Innovation Services
Biotech Commons
dmanuel@pobox.upenn.edu
Lynda Kellam, MA, MLIS, PhD
Head of Research Data Services
lmkellam@upenn.edu
Varvara Kountouzi, MS, MsEd
Head, Judith & William Bollinger Digital Fabrication Laboratory
Holman Biotech Commons
varvarak@upenn.edu
Jennifer Lege-Matsuura, BA, MSLIS
Medical Research Librarian
jlege@upenn.edu
Table Number: 22
Live Presentation: 1:20-1:25
Penn Vet Comparative Pathology Core - RRID:SCR_022438 (Penn Vet)
The Comparative Pathology Core (CPC), an Abramson Cancer Center shared resource, provides expert pathological characterization and validation of mouse and other animal models used in biomedical research by offering the expertise of board-certified veterinary pathologists and access to state-of-the-art histology, molecular staining of animal tissues, and digital pathology services.
Enrico Radaelli, DVM, PhD, DECVP
Director
enrada@vet.upenn.edu
Charley Assenmacher, DVM, MSc, DACVP
Technical Director
chasse@upenn.edu
Table Number: 13
Live Core Facility Presentation: 11:20-11:25
Penn Vet Imaging Core - RRID:SCR_022436 (Penn Vet)
The Penn Vet Imaging Core provides access to variety of light microscopy imaging modalities, including laser scanning and spinning disk confocal, fluorescence lifetime imaging (FLIM), Multiphoton imaging (e.g. intravital, SHG, and more), TIRF, SIM super-resolution, and high content imaging and analysis.
Gordon Ruthel, PhD
Core Manager
goruthel@vet.upenn.edu
Table Number: 29
PMACS Research IS
PMACS (Penn Medicine Academic Computing Systems), was formed in 2012 with the goal of providing computing services for the Perelman School of Medicine’s departments, centers, and institutes. PMACS has matured into a 140+ department of technology professionals providing a wide range of services including:
-Desktop Support
-Server Administration
-Storage Management
-Both High Performance Computing (HPC) and Limited Performance Computing (LPC)
-Software Development
-Web Design and Development
-Database Development
-Enterprise Application Development and Support
-Consulting services that provide options to best support research initiatives
PMACS is focused on delivering state of the art technology solutions in the most cost-conscious manner in support of the education, administrative and research needs of the Perelman School of Medicine.
CRIS
Aaron Atkinson
Information Technology Project Leader Senior
aatkins2@upenn.edu
Ralph Johnson
Senior Application Analyst
ralph.johnson@pennmedicine.upenn.edu
LIMS
Kaitlyn Phelan
Lead Application Analyst
kaitlyn.phelan@pennmedicine.upenn.edu
Table Number: 19
Proteomics & Metabolomics Facility (Wistar)
The Wistar Proteomics & Metabolomics Facility provides high sensitivity proteomics, metabolomics, and lipidomics analyses using state-of-the-art mass spectrometry instruments and methods. Please see website for details.
Hsin-Yao Tang, PhD
Scientific Director
tangh@wistar.org
Aaron Goldman, PhD
agoldman@wistar.org
Table Number: 1 & 2
Qualitative Research Core (CHOP)
The Qualitative Research Core is dedicated to supporting qualitative and mixed-method research. We support researchers with defining research questions, conceptualizing sampling strategies, selecting and designing appropriate data collection methods, and executing qualitative work.
Katherine Kellom, BA
Director
kellomk@chop.edu
Table Number: 23
Raymond G. Perelman Center for Cellular and Molecular Therapeutics Research Viral Vector Core - RVC Core (CHOP)
The Research Vector Core provides state-of-the-art technology support for investigators requiring viral-based vectors for gene transfer in basic research and pre-clinical studies. The core utilizes molecular biology techniques and follows Good Laboratory Practice (GLP) guidelines to engineer and produce premium quality recombinant AAV and Lentiviral (LV) vectors at a variety of scales. The core works closely with investigators to develop vectors for individual experiments.
Xueyuan Liu, MS
Director
liuxu@chop.edu
Table Number: 26
Research Instrumentation Shop (RIS) - RRID:SCR_022424 (PSOM)
The Research Instrumentation Shop (RIS) is a non-profit, shared resource machine shop facility of the Perelman School of Medicine at the University of Pennsylvania. Our staff is comprised of mechanical specialists with years of experience in the design and construction of custom laboratory and experimental clinical instrumentation. RIS has a long history of assisting researchers and graduate students from Penn, as well as scientists outside the University and from industry, with a wide variety of disciplines and perspectives. RIS will meet their needs to design, construct, and package their research instrumentation in a timely fashion. We are conveniently located in the basement of the John Morgan Building, Room 75. Please visit us to discuss your research requirements.
Dieter Hunt
Machinist
dieter.hunt@pennmedicine.upenn.edu
Table Number: 31
RIA Biomarker Core Facility - RRID:SCR_022405 (PSOM)
The Radioimmunoassay and Biomarkers Core offers a large number of high quality immunoassay and biochemistry services to basic, translational, and clinical investigators performing diabetes, obesity and related metabolic disease research. The RIA/Biomarkers Core is housed within the Institute for Diabetes, Obesity & Metabolism (IDOM) located on the 12th floor of the Smilow Center for Translational Research (room 12-183). This location places the Core on the same floor and in close proximity to the Islet Cell Biology Core, Functional Genomics Core, and Rodent Metabolic Phenotyping Core that enhances the already close interactions among these DRC Scientific Cores.
Heather Collins, PhD
Technical Director
hwc@pennmedicine.upenn.edu
Table Number: 25
Rodent Metabolic Phenotyping Core - RRID:SCR_022427 (PSOM)
The mission of the Rodent Metabolic Phenotyping Core is to provide the necessary resources and expertise to allow investigators to perform state-of-the-art studies of metabolism in rodent models.
Joe Baur, PhD
Director
baur@pennmedicine.upenn.edu
Paul Titchenell, PhD
Associate Director
ptitc@pennmedicine.upenn.edu
Corey Holman
Technical Director
holmanc@pennmedicine.upenn.edu
Table Number: 14
Live Core Facility Presentation: 11:40-11:45
Single Cell Technology Core (CHOP)
As part of the Center for Single Cell Biology, our service core provides convenient access to cutting-edge single-cell, spatial and in situ technologies to CHOP, UPenn and the wider research community. Established in early 2022, we are equipped with state-of-the-art instruments, experienced research assistants and bioinformaticians to offer in-house experimental and computational support to your single-cell research. Our services include: 10x Genomics single cell assays, 10x Genomics Visium CytAssist assays, NanoString GeoMx Digital Spatial Profiler RNA and protein assays, Akoya PhenoCycler-Fusion spatial proteomics, and Vizgen MERSCOPE spatial transcriptomics.
We thrive in providing high-quality and cost-effective service work. We are happy to consult with you on how our services can facilitate your research.
Mei Zhang, PhD
Director
zhangm5@chop.edu
3501 Civic Center Blvd, CTRB A450D
Philadelphia, PA 19104
Table Number: 30
Live Core Facility Presentation: 1:40-1:45
Small Animal Imaging Facility (CHOP)
The Small Animal Imaging Facility (SAIF) at Children’s Hospital is specialized in multi-modality radiological imaging for mice and rats. We provide a clean and state-of-the-art environment to conduct the imaging required for your longitudinal studies.
SAIF provides:
• Magnetic Resonance Imaging (MRI)
• Positron Emission Tomography/Computed Tomography (PET/CT)
• Optical Imaging
• Ultrasound
• Microimaging and NMR
The core conducts research in all areas of preclinical anatomical imaging, functional imaging, and molecular imaging. The core will assist with the design and execution of your study and help with the interpretation/analysis of imaging data.
Sergey Magnitsky, PhD
Technical Director
magnitsksm@email.chop.edu
Table Number: 8
Live Core Facility Presentation: 10:40-10:45
Small Molecule and Metabolite Core Laboratory (CHOP)
Provide assay development, validation and implementation for bioanalysis of drugs, metabolites and biomarkers utilizing liquid chromatography-tandem mass spectrometry, gas chromatography mass spectrometry and isotope ratio mass spectrometry systems.
Stephen Master, MD, PhD
Director
masters@chop.edu
James Bruton
Laboratory Manager
brutonj@chop.edu
Table Number: 13
Stem Cell & Xenograft Core Facility - RRD:SCR_010035 (PSOM)
The Stem Cell and Xenograft Core (SCXC) is a comprehensive resource laboratory and was established at the Perelman School of Medicine in 2008. The mission of the Core is to provide Investigators with a high-quality, cost-effective, and comprehensive resource to promote basic and translational research in the areas of cancer biology, infectious disease, immunotherapy, stem cell biology, and regenerative medicine. We offer services centered around 2 components: (1) An extensive repository of live and fully annotated cells from adult patients with hematologic malignancies (AML, ALL, CLL, MPN, MDS, MM), and hematopoietic stem/progenitor cells from healthy donors. (2) A full array of in vivo services and xenograft models (PDX, humanized immune system), in a dedicated BSL-2 barrier space equipped with optical imaging. The SCXC remains unique amongst peer institutions with regards to the scopes of research, its scale, and its range of services.
Nicolas Skuli, PhD
Director
nicskuli@pennmedicine.upenn.edu
Table Number: 3
Transgenic & Chimeric Mouse Core Facility (TCMF) RRID:SCR_022388 (PSOM)
The Transgenic and Chimeric Mouse Facility provides a centralized service to efficiently produce infection-free transgenic, chimeric, and genome-edited strains of mice. These mice carry randomly inserted transgenes and/or site-specific alterations in the mouse genome of specific interest to Penn researchers. The Facility offers services including DNA pronuclear injection into fertilized oocytes (along with genotyping of transgenic founders), ES cell injection into blastocysts, cytoplasmic/pronuclear injections into fertilized oocytes of CRISPR-Cas9 mix (gRNA, Cas9RNA, ssDNA/dsDNA templates), embryo and sperm cryopreservation, in vitro fertilization, and re-derivation of live and cryopreserved lines. The Core also oversees a cyropreservation facility for long-term storage of mouse embryos and sperm samples.
Douglas Epstein, PhD
Facility Director
epsteind@pennmedicine.upenn.edu
Kathleen Moosbrugger
Research Specialist
kthompso@pennmedicine.upenn.edu
Table Number: 5
Transgenic Mouse Core Facility - RRID:SCR_022441 (Penn Vet)
The Transgenic Mouse Core located in the School of Veterinary Medicine. We offer a full line of embryological manipulation services. These services primarily enable the generation of genetically modified murine models via Crispr and ES-cell injection as well as re-derivation via ICSI and IVF.
Adrian Leu
Director
nleu@vet.upenn.edu
Stephanie Sterling
Laboratory Manager
sterlin@vet.upenn.edu
Table Number: 7
Translational Core Laboratory - TCL (CHOP)
The Translational Core Laboratory (TCL) at the Children’s Hospital of Philadelphia (CHOP) Research Institute supports basic, pre-clinical, and clinical research for investigators from CHOP, the University of Pennsylvania, and external sites. The CHOP TCL performs immunoassays for the quantitation of protein/peptide biomarkers, cytokines, hormones, and antibodies in various body fluids. The TCL also performs blood-chemistry assays and hematology assays. Over the years, TCL has gathered a breadth of instruments for protein biomarker detection via immunoassay, including clinical grade, research grade, and multiplex. This has made us a “One-Stop Shop" for protein biomarker detection on the CHOP campus. We can test numerous biomarkers and provide a balance in cost, quality and speed.
David Stokes, PhD
Technical Director
stokesdg@chop.edu
Nkecha Hughes, MS
Lab Manager
HughesN@chop.edu
Table Number: 3
Tumor Tissue & Biospecimen Bank - RRID:SCR_022430 (PSOM)
The Tumor Tissue and Biospecimen Bank TTAB is a centralized biorepository of human biosamples. TTAB is responsible for the collection, processing, and storage of human blood, fluid, and tissue at the University of Pennsylvania Health System. TTAB has a collection bench within the Surgical Pathology Suite at the Hospital of the University of Pennsylvania (HUP). Our collection bench sits adjacent to the frozen section teams managing clinical sample collection and allowing for tight integration of the tissue sample collection with the clinical pathology teams.
Federico Valdivieso, BS
Technical Director
valdivie@mail.med.upenn.edu
Table Number: 7
University Laboratory Animal Resources - ULAR (Penn)
ULAR services and responsibilities include:
1. Daily husbandry care, including feeding and watering, for both routine and special requirements, cleaning and monitoring for the health of the animals from six colleges at UPenn, 24/7/365
2. Clinical care of animals with both spontaneous and protocol related health issues
3. Consultation on IACUC protocols and animal models
4. A Quality Assurance program to ensure the animals are healthy and free of infectious diseases
5. Researcher training, teaching the UPenn community both basic handling and advanced procedures for all of the species at the university
6. Research support, including veterinarians and veterinary technicians providing both surgical and anesthetic support and renting commonly required anesthetic and monitoring equipment
7. Pathology services working with research groups in the study of both research models and spontaneous diseases within the colonies
8. Business and procurement office, which helps groups with both procuring animals and billing for animal related services
9. Resources for researchers to prevent compassion fatigue and other wellness issues associated with working with animals in biomedical research
James Marx, DVM, PhD, DACLAM
marx@upenn.edu
Table Number: 16
Vector Core Facility - RRID:SCR_022432 (PSOM)
The Penn Vector Core is a full-service viral-vector core facility with more than a decade of experience in manufacturing. The Core's main objective is to provide investigators access to vector technology for preclinical studies and other basic research applications involving gene therapy or gene transfer. We specialize in the provision of novel AAV serotype vectors, some of which were developed here at Penn.
Kenton Woodard, PhD
Director
kentonw@upenn.edu
Bomi Adejuwon, MS
Associate Director, Preclinical Vector
Adebomi.Adejuwon@pennmedicine.upenn.edu
Table Number: 24
Viral Molecular High Density Sequencing Core - RRID:SCR_022433
The viral molecular high density sequencing (VMHDS) core offer services to assist in evaluating cell and gene therapies (lentivirus, MLV, AAV, CRISPR). We are currently assisting with over 20 gene therapy clinical trials globally across academic and corporate institutions, as both exploratory and safety endpoints, and have contributed to more than 80 publications. We are experts in molecular analyses, custom informatics and assisting with regulatory approval documents.
Aoife Doto, PhD
Technical Director
rochea@pennmedicine.upenn.edu
Table Number: 35
Live Core Facility Presentation: 12-12:05
Wistar Animal Facility - WAF (Wistar)
The Wistar Animal Facility is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC), a private, nonprofit organization that promotes the humane treatment of animals in science through voluntary accreditation and assessment programs. Expert management, husbandry, custodial and veterinary staff maintain the facility, including a modified Biosafety Level 2 (BSL2) barrier
Denise DiFrancesco, AS, RLATG, CMAR, ILAM
Managing Director
difrancesco@wistar.org
Marci Houston Leslie, BA, LAT
Supervisor
mhouston@wistar.org
Table Number: 1 & 2
Zebrafish Core (CHOP)
The Zebrafish Core offers all resources for zebrafish research and provides fish husbandry. We design and perform experiments, teach zebrafish techniques and advice and support researchers. Our special expertise is disease modeling, analysis of disease phenotypes, and drug testing.
Christoph Seiler, PhD
Director
seilerc@chop.edu
Table Number: 15
Live Core Facility Presentation: 11:30-11:35