Core Description
The Metabolomics Core provides expertise to quantitate metabolites in biological samples using liquid chromatography/mass spectrometry. Our mission is to perform the assays and assist in the interpretation of the results. The core is available to the entire Penn research community, external research investigators, and industry
Services:
Custom LC/MS Assay Method Development
One of our key strengths is custom LC/MS assay method development for metabolites. There are 3 levels of method development (low, medium, and high difficulty) with a set fee for each.
Untargeted LC/MS Metabolomics
Untargeted metabolomics is performed on a Thermo Orbitrap ID-X mass spectrometer to identify metabolites (MW 60-1000) for biomarker discovery. Each sample is run through 2 separate LC/MS methods (reversed-phase C18 chromatography to retain medium polarity to non-polar metabolites and HILIC chromatography to retain highly polar metabolites). Statistical data analysis includes PCA, whisker plots, heat maps, and a database search to identify metabolites.
Targeted Metabolomics Assay Modules
Targeted metabolomics assays are available for the quantitative measurement of a wide variety of metabolites in cellular extracts, plasma, or tissues using liquid chromatography/mass spectrometry. These assays provide crucial information about metabolic pathways involving the three major classes of nutrients: lipids, carbohydrates, and proteins. Our assays include the quantitation of:
- Acylcarnitines (products of fatty acid oxidation)
- Amino Acids (products of fatty acid and amino acid oxidation)
- Organic acids (TCA cycle intermediates; products of glucose and amino acid oxidation)
- Malonyl and Acetyl CoA
- Nucleotides
- Catecholamines and Metanephrines
Quantitation of these metabolites by liquid chromatography/mass spectrometry (Agilent 1290/6495 triple quadrupole mass spectrometer) is achieved by addition of isotopically-labelled internal standards to cells, biological fluids, and tissues from an array of model organisms and human subjects.
Acylcarnitines (57)
| C2 | C8 | C4:1 | C18:2 | C22:4 | C5-DC | C16-OH | C20:2-OH |
| C3 | C10 | C5:1 | C20:1 | C22:5 | C6-OH | C16:1-OH | |
| C4-butyryl | C12 | C12:1 | C20:2 | C3-DC | C8-OH | C16:2-OH | |
| C4-isobutyryl | C14 | C14:1 | C20:3 | C4-OH butyryl | C8:1-OH | C18-OH | |
| C5-valeryl | C16 | C14:2 | C20:4 | C4-OH isobutyryl | C12-OH | C18:1-OH | |
| C5-isovaleryl | C18 | C16:1 | C22:1 | C4-DC succinyl | C14-OH | C18:2-OH | |
| C5 2-Me-butyryl | C20 | C16:2 | C22:2 | C4-DC Me-malonyl | C14:1-OH | C20-OH | |
| C6 | C22 | C18:1 | C22:3 | C5-OH | C14:2-OH | C20:1-OH |
Amino Acids (30)
| Gly | Lys | His | Trp | Val | Leu | Asn | Thr | Pro | Ser |
| Ala | Gln | Phe | Orn | Arg | Ile | Asp | Met | Tyr | Cit |
| Glu | 1-MeHis | 3-MeHis | homoCit | ADMA | SDMA | NMMA | homoArg | kynurenine | OH-kynurenine |
Nucleotides (30)
| NAM | 1-MeNAM | NMN | NAMN | NAAD | NAD | NADP | NADH |
| NADPH | AMP | ADP | ATP | cAMP | GMP | GDP | GTP |
| cGMP | CMP | CDP | CTP | TMP | TDP | TTP | UMP |
| UDP | UTP | IMP | IDP | ITP | ADPR |
Organic Acids (8) |
|||||||
| lactate | pyruvate | succinate | 3-HBA | alpha-KG | malate | citrate | fumarate |
Acyl Coenzyme As (2) |
|
| malonyl CoA | acetyl CoA |
Catecholamines and Metanephrines (6)
| dopamine | epinephrine | norepinephrine | 3-methoxytyramine | metanephrine | normetanephrine |
Protocols: Cell and Tissue Harvest Protocols
Adherent Cell Harvest for Metabolomics
Cell Suspension Harvest for Metabolomics
Tissue Collection Protocol for Targeted Metabolomics
Service Requests
Instructions to Register and Request iLab Services
Please acknowledge the Penn Metabolomics Core in your publications:
Metabolomics studies were performed by the Penn Metabolomics Core (RRID:SCR_022381) supported by the Penn Cardiovascular Institute and, in part, by NCI P30 CA016520 and NIH P30DK050306.
Contact
Chris Petucci, Ph.D.
Director
Email: Christopher.Petucci@pennmedicine.upenn.edu
Phone: 215-898-9765
Location: Smilow TRC 9-196
Clarissa Shoffler, B.S.
Research Specialist
Email: Clarissa.Shoffler@Pennmedicine.upenn.edu
Phone: 215-746-4313
Location: Smilow TRC 9-196
Dina Abbasian, B.S.
Research Specialist
Email: Dina.Abbasian@Pennmedicine.upenn.edu
Phone: 215-746-4313
Location: Smilow TRC 9-196
Zoltan P. Arany, M.D., Ph.D., Faculty Core Advisor
Email: zarany@pennmedicine.upenn.edu
Location: Smilow TRC 11-106
Daniel Kelly, M.D., Faculty Core Advisor
Email: dankelly@pennmedicine.upenn.edu
Location: Smilow TRC 11-122