The Metabolomics Core provides expertise in untargeted and targeted metabolomics using liquid chromatography/mass spectrometry. Our mission is to perform the assays and assist in the interpretation of the results. The core is available to the entire Penn research community, external research investigators, and industry.
Custom LC/MS Assay Method Development
One of our key strengths is custom LC/MS assay method development for metabolites. There are 3 levels of method development (low, medium, and high difficulty) with a set fee for each.
Untargeted LC/MS Metabolomics
Untargeted metabolomics is performed on a Thermo Orbitrap ID-X mass spectrometer to identify metabolites (MW 60-1000) for biomarker discovery. Each sample is run through 2 separate LC/MS methods (reversed-phase C18 chromatography to retain medium polarity to non-polar metabolites and HILIC chromatography to retain highly polar metabolites). Statistical data analysis includes PCA, whisker plots, heat maps, and a database search to identify metabolites.
Targeted Metabolomics Assay Modules
Targeted metabolomics assays are available for the quantitative measurement of a wide variety of metabolites in cellular extracts, plasma, or tissues using liquid chromatography/mass spectrometry. These assays provide crucial information about metabolic pathways involving the three major classes of nutrients: lipids, carbohydrates, and proteins. Our assays include the quantitation of:
- Acylcarnitines (products of fatty acid oxidation)
- Amino Acids (products of fatty acid and amino acid oxidation)
- Organic acids (TCA cycle intermediates; products of glucose and amino acid oxidation)
- Malonyl and Acetyl CoA
Quantitation of these metabolites by liquid chromatography/mass spectrometry (Agilent 1290/6495 triple quadrupole mass spectrometer) is achieved by addition of isotopically-labelled internal standards to cells, biological fluids, and tissues from an array of model organisms and human subjects.
|C5 2-Me-butyryl||C20||C16:2||C22:2||C4-DC Me-malonyl||C14:1-OH||C20-OH|
Amino Acids (30)
Organic Acids (8)
Acyl Coenzyme As (2)
|malonyl CoA||acetyl CoA|
Protocols: Cell and Tissue Harvest Protocols
Chris Petucci, Ph.D.
Location: Smilow TRC 9-196
Zoltan P. Arany, M.D., Ph.D., Faculty Core Advisor
Location: Smilow TRC 11-106
Daniel Kelly, M.D., Faculty Core Advisor
Location: Smilow TRC 11-122