Perelman School of Medicine at the University of Pennsylvania

Cardiovascular Institute (CVI)

Metabolomics Core

Core Description

The Metabolomics Core provides expertise in untargeted and targeted metabolomics using liquid chromatography/mass spectrometry. Our mission is to perform the assays and assist in the interpretation of the results. The core is available to the entire Penn research community, external research investigators, and industry.


Services:

Custom LC/MS Assay Method Development

One of our key strengths is custom LC/MS assay method development for metabolites.  There are 3 levels of method development (low, medium, and high difficulty) with a set fee for each.

Untargeted LC/MS Metabolomics

Untargeted metabolomics is performed on a Thermo Orbitrap ID-X mass spectrometer to identify metabolites (MW 60-1000) for biomarker discovery.  Each sample is run through 2 separate LC/MS methods (reversed-phase C18 chromatography to retain medium polarity to non-polar metabolites and HILIC chromatography to retain highly polar metabolites). Statistical data analysis includes PCA, whisker plots, heat maps, and a database search to identify metabolites. 

Targeted Metabolomics Assay Modules

Targeted metabolomics assays are available for the quantitative measurement of a wide variety of metabolites in cellular extracts, plasma, or tissues using liquid chromatography/mass spectrometry. These assays provide crucial information about metabolic pathways involving the three major classes of nutrients:  lipids, carbohydrates, and proteins. Our assays include the quantitation of:

  • Acylcarnitines (products of fatty acid oxidation)
  • Amino Acids (products of fatty acid and amino acid oxidation)
  • Organic acids (TCA cycle intermediates; products of glucose and amino acid oxidation)
  • Malonyl and Acetyl CoA
  • Nucleotides

Quantitation of these metabolites by liquid chromatography/mass spectrometry (Agilent 1290/6495 triple quadrupole mass spectrometer) is achieved by addition of isotopically-labelled internal standards to cells, biological fluids, and tissues from an array of model organisms and human subjects.

 

Acylcarnitines (57)
C2 C8 C4:1 C18:2 C22:4 C5-DC C16-OH C20:2-OH
C3 C10 C5:1 C20:1 C22:5 C6-OH C16:1-OH  
C4-butyryl C12 C12:1 C20:2 C3-DC C8-OH C16:2-OH  
C4-isobutyryl C14 C14:1 C20:3 C4-OH butyryl C8:1-OH C18-OH  
C5-valeryl C16 C14:2 C20:4 C4-OH isobutyryl C12-OH C18:1-OH  
C5-isovaleryl C18 C16:1 C22:1 C4-DC succinyl C14-OH C18:2-OH  
C5 2-Me-butyryl C20 C16:2 C22:2 C4-DC Me-malonyl C14:1-OH C20-OH  
C6 C22 C18:1 C22:3 C5-OH C14:2-OH C20:1-OH  
Amino Acids (30)
Gly Lys His Trp Val Leu Asn Thr Pro Ser
Ala Gln Phe Orn Arg Ile Asp Met Tyr Cit
Glu 1-MeHis 3-MeHis homoCit ADMA SDMA NMMA homoArg kynurenine OH-kynurenine
Nucleotides (30)
NAM 1-MeNAM NMN NAMN NAAD NAD NADP NADH
NADPH AMP ADP ATP cAMP GMP GDP GTP
cGMP CMP CDP CTP TMP TDP TTP UMP
UDP UTP IMP IDP ITP ADPR    
Organic Acids (8)
lactate pyruvate succinate 3-HBA alpha-KG malate citrate fumarate
Acyl Coenzyme As (2)
malonyl CoA acetyl CoA

Protocols: Cell and Tissue Harvest Protocols

Metabolite Extraction Protocol for Cell-Based Experiments

Tissue Collection Protocol for Targeted Metabolomics


Service Requests

Instructions to Register and Request iLab Services for University of Pennsylvania Researchers

Instructions to Register and Request iLab Services for External Researchers


Contact

Chris Petucci, Ph.D.

Director
Email: Christopher.Petucci@pennmedicine.upenn.edu
Phone: 215-898-9765
Location: Smilow TRC 9-196

Zoltan P. Arany, M.D., Ph.D., Faculty Core Advisor

Email: zarany@pennmedicine.upenn.edu
Location: Smilow TRC 11-106

Daniel Kelly, M.D., Faculty Core Advisor

Email: dankelly@pennmedicine.upenn.edu
Location: Smilow TRC 11-122