The Rodent Cardiovascular Physiology Core provides services to assess cardiovascular function in mouse models and to provide validated surgical models of heart and vascular disease in mice to assess genetic or therapeutic interventions. We will work with investigators to design and implement the study along with any associated grant applications, animal protocol submissions and manuscript preparation. While the central focus of the facility is cardiovascular research, the techniques employed are often useful to investigators in other fields. Our Core staff will work with you to assess your needs and provide the necessary technical training and scientific assistance in animal protocol preparation. The Core runs on a fee-for-service model. Accordingly, investigators will be responsible for the costs incurred for their projects and prior animal protocol approval by Penn IACUC.
Staff:
Ling Lai, M.D., Ph.D.
Director and Cardiac Surgeon
Phone: 215-898-2656
Email: linglai@pennmedicine.upenn.edu
Surgical Suite: Smilow 11-222
Joanna Griffin, MS, RCS, RDCS
Rodent Echocardiographer
Phone: 215-573-3913
Email: Joanna.Griffin@Pennmedicine.upenn.edu
Echo Lab: Smilow 11-222
Ingrid Marti Pamies, PhD
Mouse Echocardiographer (part-time)
Email: ingridm@pennmedicine.upenn.edu
Marielle Scherrer-Crosbie, MD, PhD
Faculty Core Advisor for Echocardiography
Email: Marielle.Scherrer-Crosbie@pennmedicine.upenn.edu
Office: South Pavilion 11-131
Daniel P. Kelly, MD
Faculty Core Advisor for Cardiac Surgery and Physiology
Email: dankelly@pennmedicine.upenn.edu
Office: Smilow TRC 11-122
Operations:
Teresa C. Leone
Associate Director of Scientific Administration and Operations, CVI
Phone: 215-898-0768
Email: terleone@pennmedicine.upenn.edu
Office: Smilow 11-122A
Cost is per mouse/per time point. Non-invasive single transthoracic cardiac ultrasound exam performed under Avertin (tribromoethanol)-induced anesthesia.
Users can consider A for screening mice for homogenous decreases in function /hypertrophy (e.g. sepsis, etc.). Consider D in mice with regional wall motion abnormalities. Note that M Mode parameters do not accurately represent LV structure and function.
- SCREENING ECHO
Acquisition and analysis of M-Mode images for LV function (fractional shortening), chamber dimensions, and wall thickness. Ideal for initial assessment of genetically-engineered mouse models. - SCREENING PLUS ECHO
Everything screening echo includes, plus acquisition and analysis of 2 dimensional images for LV end-diastolic and end-systolic volumes and ejection fraction. - ECHO for Advanced Diastolic Function Analysis*
Acquisition and analysis of 2D, M-Mode, and Doppler images to assess diastolic function including mitral valve inflow, tissue Doppler imaging, pulmonary vein flow, and cardiac strain (LV volumes, EF, LV Mass). - TAC ECHO
Screening Plus echo (M-Mode, volumes, EF) in addition to aortic trans-banding gradient assessment. - MI ECHO
Acquisition of 2D long axis images for cardiac strain (LV volumes, EF, LV Mass) and 2D short axis serial imaging slices for regional wall motion analysis. - TAC+ MI (Heart Failure) ECHO
MI echo plus aortic trans-banding gradient assessment. - Right heart assessment (PAB ECHO)
Acquisition of 2D and Doppler images for assessment of RV area, RV free wall thickness, RV tissue Doppler imaging, TAPSE, tricuspid regurgitation, and pulmonary artery band gradient.
*Consultation with Core required prior to scheduling.
- TRANSVERSE AORTIC CONSTRICTION (TAC)- Compensated LV Hypertrophy Model
Operative placement of constriction on transverse aorta that results in left ventricular hypertrophy with normal systolic function in timeframe of 1-4 weeks. Timing of endpoint determined by scientific question. Recommend M-mode echo with gradient at endpoint. - MYOCARDIAL INFARCTION (MI)
Operative placement of a permanent occlusion around the left anterior descending artery of the mouse heart. Includes recording mouse weight. Size of MI can be customized but is typically sufficient size to impact LV function. Recommended Complete 2D echo and collection of endpoints at 1-2 weeks post-surgery. - OPEN CHEST ISCHEMIA REPERFUSION INJURY (OCIR)
Operative placement of an occluder around the left anterior descending artery for 30-45mins of occlusion followed by reperfusion for typically 24hrs. Vital dye staining will also be performed which involves euthanasia and placement of infusion line in aorta, and ligation of innominate artery, carotid artery and left subclavian artery. Heart will then be perfused with tetrazolium chloride and Evans Blue, with analysis to determine infarcted area and “area at risk”. - COMBINED TRANSVERSE AORTIC CONSTRICTION (TAC)/MYOCARDIAL INFARCTION (MI) – Heart Failure (HF) Model
Operative placement of constriction on the transverse aorta combined with a small myocardial infarction caused by permanent ligation of the distal third of the left anterior descending artery. Heart Failure (global LV dilatation and remodeling) will typically progress over a 4 week period. Recommend Complete 2D echo at endpoint. - PULMONARY ARTERY BANDING (PAB)
Operative placement of constriction on the pulmonary artery. RV dilation, dysfunction, and tricuspid regurgitation will typically progress over a 7-10 day period. We recommend a PAB echo be performed at day 10 post surgery. - PRE-WEANING AORTIC BANDING (PWAB)
Operative placement of constriction on the ascending aorta at 3 weeks of age. PWAB results in left ventricular hypertrophy. Recommended 8-10 week post surgery echo with systolic and diastolic assessment.
OTHER SERVICES
- INJECTIONS OF PHARMACOLOGICAL AGENTS
- JUGULAR CATHETERIZATION (for drug infusion)
- ALZET PUMP IMPLANTATION
- TELEMETRY IMPLANTATION
- ORGAN HARVEST
- HEMODYNAMICS I
Millar catheter placement, in carotid/aorta and recording of arterial or aortic pressures only. - HEMODYNAMICS II
Millar catheter placement in left or right ventricle, and derivation of ventricular peak pressures, ventricular end-diastolic pressure, +/- dP/dt, and tau. - HEMODYNAMICS III
Millar catheter placement into left ventricle and recording of LV measurements as in “Hemodynamics II” during pharmacological intervention (Dobutamine Challenge). - HEMODYNAMICS IV
Millar catheter placement into left ventricle, Pressure-Volume loop recording including all measurements of “Hemodynamics II”.
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Request Surgical, Echo, and Hemodynamics Services
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Prices for Surgical, Echo, and Hemodynamics Services
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CVI Rodent Cardiovascular Phenotyping Core Mouse and Data Transfer Logsheet
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Slides from Rodent Cardiovascular Phenotyping Core Presentation
Other services are available upon request. Please email terleone@pennmedicine.upenn.edu.
Please remember to acknowledge the RCPC in your publications as follows:
- For cardiac/hemodynamics services: The cardiac surgeries (or hemodynamic studies) were performed by the Rodent Cardiovascular Phenotyping Core (RRID: SCR_022419) at the University of Pennsylvania supported by the Penn Cardiovascular Institute.
- For echo services: The echocardiography were performed by the Rodent Cardiovascular Phenotyping Core (RRID: SCR_022419) at the University of Pennsylvania supported by the Penn Cardiovascular Institute and NIH S10OD016393.
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