Perelman School of Medicine at the University of Pennsylvania

Stem Cell & Xenograft Core

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Stem Cell & Xenograft Core

The Stem Cell and Xenograft Core (SCXC) is a comprehensive resource laboratory integrating a viable tissue bank of normal and malignant human hematopoietic cells with a full range of xenograft models and services.

The SCXC is committed to providing users with viable primary human hematopoietic tissues and promote translational research. Our core can provide bone marrow from healthy adult donors and umbilical cord blood. Mononuclear and CD34+cells from healthy bone marrow and cord blood are available, and other cell fractions can be provided by arrangement.

In addition, we maintain a large tissue bank of viable cells from hematopoietic malignancies including AML, ALL, CML, MDS and MPDs. All samples are fully annotated and frozen as viable cells. We also offer access to an immunomagnetic cell sorter (MiltneyiAutoMacs). Expertise in primary human hematopoietic stem/progenitor and leukemic cell culture and in vitro assays is available. For consultation or questions, please contact Martin Carroll.

The SCXC offers a wide variety of xenograft services from training to full-service experiments. The Core maintains a large breeding colony of immune-deficient (NSG) mice and experimental animals are housed in an NSG-dedicated suite fully equipped for routine procedures, survival surgeries and optical imaging systems. 

We offer we wide variety of xenograft models ranging from human immune (CD34-transplant or BLT) reconstituted NSG mice for immuno-oncology and infectious disease studies, to orthotopic patient-derived xenograft (PDX) models of hematologic malignancies (AML, ALL) and solid tumors (ovarian, hepatic, brain, lung).  Our Core has expertise in xenograft models for regenerative medicine studies including human liver reconstitution (FRG model), human iPS and ES cell in vivo testing and human islet transplantation. For consultation or questions, please contact Gwenn Danet-Desnoyers.