CVI Program Unit(s):
Cardiovascular Development / Congenital Heart Disease
Myocyte Biology / Heart Failure
Thrombosis / Hemostasis
CVI Research Description:
We are working on the role of a poorly understood posttranslational modification -- arginylation -- in cardiovascular development. Arginylation is mediated by arginyltransferase Ate1. Its knockout leads to embryonic lethality in mice and severe cardiovascular defects. My lab is focused on uncovering the underlying mechanisms of these defects on the molecular, cellular, and organizmal levels.
Wong,CCL.; XU,T.; Rai,R.; Bailey,A.O.; Yates,J.R.,III; Wolf,Y.I.; Zebroski,H.; Kashina,A.: Global analysis of posttranslational protein arginylation. PLoS Biology 5(10):e258, 2007.
Rai R, Mushegian A, Makarova K, Kashina A: Molecular dissection of arginyltransferases guided by similarity to bacterial peptidoglycan synthases. EMBO Rep 2006 Notes: On line: July 7, 2006.
Kashina,A.S.: Differential arginylation of actin isoforms: the myster of the actin N-terminus. Trends Cell Biology 16: 610-615, 2006 Notes: On line: Oct. 11, 2006.
Karakozova M, Kozak, M, Wong, C. C. L., Bailey, A. O., Yates, J. R, III, Mogilner, A., Zebroski, H., and Kashina, A. : Arginylation of Beta Actin Regulates Actin Cytoskeleton and Cell Motility. Science 2006 Notes: On line: June 22, 2006.
R. Rai and A. Kashina : Identification of mammalian arginyltransferases that modify a specific subset of protein substrates. Proc. Natl. Acad. Sci., USA 102: 10123-10128 2005.
A. Kashina and V. Rodionov : Intracellular Organelle Transport: Few Motors, Many Signals. Trends in Cell Biology 15: 396-398, 2005.
I. Sorokina and A. Kashina : Archived gels as a tool for identification of protein complexes: POLO kinase cofractionates with Drosophila 205 kDa MAP and ncd in mitotic embryonic extracts. Analytical Biochemistry 344: 55-157, 2005.
Kwon YT, Kashina AS,(equal contribution), Davydov IV, Hu RG, An JY, Seo JW, Du F, Varshavsky A.: An essential role of N-terminal arginylation in cardiovascular development. Science 297: 5578-5596, 2002.
Y.T.Kwon, A.S.Kashina, (equal contribution), and A.Varshavsky: Alternative splicing results in differential expression, activity and localization of the two forms of Arginyl-tRNA-Protein transferase, a component of the N-End Rule Pathway. Molecular Cell Biology 19: 182-193, 1999.
A.S.Kashina, G.C.Rogers, and J.M.Scholey: The bimC subfamily of kinesins: essential bipolar mitotic motors driving centrosome separation. Biochem. Biophys. Acta 1357: 257-271, 1997.
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Last updated: 07/13/2015
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