Paul A. Janmey, Ph.D.
University of Pennsylvania
1010 Vagelos Research Laboratories
3340 Smith Walk
Philadelphia, PA 19104-6383
Fax: 215 573-6815
A.B. (Chemistry and Philosophy)
Oberlin College, 1976.
Ph.D. (Physical Chemistry)
University of Wisconsin, 1982.
Description of Research ExpertiseResearch Interests
cytoskeleton, phosphoinositide signalling, cell mechanics.
Key words: actin, PIP2, cytoskeleton, gel, astrocyte, fibrin, gelsolin.
Description of Research
Our lab studies several aspects of cell mechanics. In one project, we produce soft materials, usually hydrogels, to which cell adhesion proteins are linked to study how the stiffness of surfaces alters cell structure, function, and growth. Endothelial cells, fibroblasts, neurons and astrocytes each show unique dependence on substrate stiffness, and we seek to understand how they sense and respond to this mechanical cue. In related work we measure the structure and mechanics of cytoskeletal polymers using a variety of imaging, scattering, and rheologic methods. Further studies examine how changes in cell membrane structure mediated by inositol phospholipids lead to production of signals that remodel the cytoskeleton.
1. Image morphology and cytoskeletal changes in GFP-actin or GFP-vimentin expressing fibroblasts, endothelial cells, and other cell types grown of soft materials.
2. Fluorescence and atomic force microscopy of purified cytoskeletal polymers and associated motor proteins.
3. Use of fibrin gels as matrices for cell culture.
Fitzroy Byfield, Elisabeth Charrier, Ph.D., LiKang Chin, Ph.D., Katrina Cruz, Sarah Kim, Tongkeun Lee, Maria Murray, Ph.D., David Slochower, Anne Van Oosten, William Yang, Sameera Zaidi
Selected PublicationsThomson DD, Wehmeier S, Byfield FJ, Janmey PA, Caballero-Lima D, Crossley A, Brand AC.: Contact-induced apical asymmetry drives the thigmotropic responses of Candida albicans hyphae. Cell Microbiol. 17(3): 342-54, Mar 2015.
Galie PA, Byfield FJ, Chen CS, Kresh JY, Janmey PA.: Mechanically stimulated contraction of engineered cardiac constructs using a microcantilever. IEEE Trans Biomed Eng 62(2): 438-42, Feb 2015.
Galie PA, van Oosten A, Chen CS, Janmey PA.: Application of multiple levels of fluid shear stress to endothelial cells plated on polyacrylamide gels. Lab Chip 15(4): 1205-12, Feb 2015.
Mohammadi H, Arora PD, Simmons CA, Janmey PA, McCulloch CA : Inelastic behaviour of collagen networks in cell-matrix interactions and mechanosensation. J R Soc Interface 12(102): 20141074, Jan 2015.
Mendez MG, Restle D, Janmey PA: Vimentin enhances cell elastic behavior and protects against compressive stress. Biophys J 107(2): 314-23, Jul 2014.
Pogoda K, Chin L, Georges PC, Byfield FJ, Bucki R, Kim R, Weaver M, Wells RG, Marcinkiewicz C, Janmey PA : Compression stiffening of brain and its effect on mechanosensing by glioma cells. New J Phys 16: 075002, Jul 2014.
Galie PA, Nguyen D-HT, Choi CK, Cohen DM, Janmey PA, Chen CS : Fluid shear stress threshold regulates angiogenic sprouting. Proc Natl Acad Sci U S A. 111(22): 7968-73, Jun 2014.
Del Campo CM, Mishra AK, Wang Y-H, Roy CR, Janmey PA, Lambright DG : Structural basis for PI(4)P-specific membrane recruitment of the Legionella pneumophila effector DrrA/SidM. Structure 22(3): 397-408, Mar 2014.
Mohammadi H, Janmey PA, McCulloch CA.: Lateral boundary mechanosensing by adherent cells in a collagen gel system. Biomaterials 35(4): 1138-49, Jan 2014.
Murray ME, Mendez MG, Janmey PA.: Substrate stiffness regulates solubility of cellular vimentin. Mol Biol Cell 25(1): 87-94, Jan 2014.