Paul A. Janmey, Ph.D.

faculty photo
Professor of Physiology
Department: Physiology

Contact information
Institute for Medicine and Engineering
University of Pennsylvania
1010 Vagelos Research Laboratories
3340 Smith Walk
Philadelphia, PA 19104-6383
Office: 215 573-7380
Fax: 215 573-6815
Education:
A.B. (Chemistry and Philosophy)
Oberlin College, 1976.
Ph.D. (Physical Chemistry)
University of Wisconsin, 1982.
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Description of Research Expertise

Research Interests
cytoskeleton, phosphoinositide signalling, cell mechanics.

Key words: actin, PIP2, cytoskeleton, gel, astrocyte, fibrin, gelsolin.

Description of Research
Our lab studies several aspects of cell mechanics. In one project, we produce soft materials, usually hydrogels, to which cell adhesion proteins are linked to study how the stiffness of surfaces alters cell structure, function, and growth. Endothelial cells, fibroblasts, neurons and astrocytes each show unique dependence on substrate stiffness, and we seek to understand how they sense and respond to this mechanical cue. In related work we measure the structure and mechanics of cytoskeletal polymers using a variety of imaging, scattering, and rheologic methods. Further studies examine how changes in cell membrane structure mediated by inositol phospholipids lead to production of signals that remodel the cytoskeleton.

Rotation Projects
1. Image morphology and cytoskeletal changes in GFP-actin or GFP-vimentin expressing fibroblasts, endothelial cells, and other cell types grown of soft materials.

2. Fluorescence and atomic force microscopy of purified cytoskeletal polymers and associated motor proteins.

3. Use of fibrin gels as matrices for cell culture.

Lab personnel:
Fitzroy Byfield, Elisabeth Charrier, Ph.D., LiKang Chin, Ph.D., Katrina Cruz, Sarah Kim, Tongkeun Lee, Maria Murray, Ph.D., David Slochower, Anne Van Oosten, William Yang, Sameera Zaidi

Selected Publications

Janmey, Paul A., Fletcher, Daniel A., Reinhart-King, Cynthia A.: STIFFNESS SENSING BY CELLS. Physiological Reviews 100(2): 695-724, 2020.

Bradley, Ryan P., Slochower, David R., Janmey, Paul A., Radhakrishnan, Ravi: Divalent cations bind to phosphoinositides to induce ion and isomer specific propensities for nano-cluster initiation in bilayer membranes. Royal Society Open Science 7(5), 2020.

Baker, Martin J., Cooke, Mariana, Kreider-Letterman, Gabriel, Garcia-Mata, Rafael, Janmey, Paul A., Kazanietz, Marcelo G.: Evaluation of active Rac1 levels in cancer cells: A case of misleading conclusions from immunofluorescence analysis. The Journal of biological chemistry 295(40): 13698-13710, 2020.

Shivers, Jordan L., Feng, Jingchen, van Oosten, Anne S. G., Levine, Herbert, Janmey, Paul A., MacKintosh, Fred C.: Compression stiffening of fibrous networks with stiff inclusions. Proceedings of the National Academy of Sciences of the United States of America 117(35): 21037-21044, 2020.

Piktel, Ewelina, Wnorowska, Urszula, Ciesluk, Mateusz, Deptula, Piotr, Prasad, Suhanya V., Krol, Grzegorz, Durnas, Bonita, Namiot, Andrzej, Markiewicz, Karolina H., Niemirowicz-Laskowska, Katarzyna, Wilczewska, Agnieszka Z., Janmey, Paul A., Reszec, Joanna, Bucki, Robert: Recombinant Human Plasma Gelsolin Stimulates Phagocytosis while Diminishing Excessive Inflammatory Responses in Mice with Pseudomonas aeruginosa Sepsis. International Journal of Molecular Sciences 21(7), 2020.

Patteson, Alison E., Vahabikashi, Amir, Goldman, Robert D., Janmey, Paul A.: Mechanical and Non-Mechanical Functions of Filamentous and Non-Filamentous Vimentin. Bioessays 2020.

Patteson, Alison E., Carroll, Bobby, Iwamoto, Daniel, Janmey, Paul A.: The vimentin cytoskeleton: When polymer physics meets cell biology. Physical biology 2020.

Moorthy, Sudha, Byfield, Fitzroy J., Janmey, Paul A., Klein, Eric A.: Matrix stiffness regulates endosomal escape of uropathogenic E. coli. Cellular Microbiology 22(5), 2020.

Mandal, Kalpana, Gong, Ze, Rylander, Alexis, Shenoy, Vivek B., Janmey, Paul A.: Opposite responses of normal hepatocytes and hepatocellular carcinoma cells to substrate viscoelasticity. Biomaterials Science 8(5): 1316-1328, 2020.

Mandal, Kalpana, Gong, Ze, Rylander, Alexis, Shenoy, Vivek B., Janmey, Paul A.: Opposite responses of normal hepatocytes and hepatocellular carcinoma cells to substrate viscoelasticity (vol 8, pg 1316, 2020). Biomaterials Science 8(7): 2040-2040, 2020.

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Last updated: 10/08/2020
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