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Francisco Gonzalez-Scarano, MD
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Emeritus Professor of Neurology
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Department: Neurology
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Contact information
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Department of Neurology
32 Hospital of the University of Pennsylvania
14 3 West Gates
3a 3400 Spruce Street
Philadelphia, PA 19104-4283
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32 Hospital of the University of Pennsylvania
14 3 West Gates
3a 3400 Spruce Street
Philadelphia, PA 19104-4283
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Office: (215) 662-3360
34 Fax: (215) 662-3362
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34 Fax: (215) 662-3362
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Publications
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Links
a4 Search PubMed for articles
42 Neuroscience graduate group faculty webpage.
4d Cell and Molecular Biology graduate group faculty webpage.
44 Immunology graduate group faculty webpage.
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a4 Search PubMed for articles
42 Neuroscience graduate group faculty webpage.
4d Cell and Molecular Biology graduate group faculty webpage.
44 Immunology graduate group faculty webpage.
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Education:
21 9 B.A. 16 (Economics) c
29 Yale University , 1971.
21 9 M.D. c
30 Northwestern University, 1975.
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Permanent link21 9 B.A. 16 (Economics) c
29 Yale University , 1971.
21 9 M.D. c
30 Northwestern University, 1975.
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3d HIV Neuropathogenesis, Neurovirulence, Bunyaviruses.
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4a Key words: HIV, microglia, neuropathogenesis, HIV Dementia.
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26 Description of Research
4c Our laboratory studies viral neurotropism, in two groups of viruses.
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29e Human immunodeficiency virus causes a dementia, in addition to its primary effects on the immune system. The projects in the laboratory are primarily oriented to defining the pathophysiology of this HIV Dementia, which may be mediated by either direct infection of astrocytes, neurons and oligodendrocytes, or more likely, by indirect effects from infection of microglia. Current projects involve the analysis of CD4 dependence of HIV strains and the potential for development of neutralization-resistant strains in the CNS environment. An SIV project will look at viral evolution within the CNS, including analysis of viral genomes at the level of single cells.
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180 La Crosse virus is a member of the Bunyaviridae, a large family of viruses that includes the hantaviruses, which cause hantavirus pulmonary syndrome. La Crosse is a common cause of pediatric encephalitis. Current La Crosse projects look at the regions of the viral glycoproteins responsible for entry into muscle cells, and at the use of siRNA to inhibit La Crosse infections.
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2e Rotation Projects for 2006-2007
3c 1. Role of NSs in inhibition of siRNA in La Crosse.
49 2. Analysis of SIV gp120 sequences recovered from CSF and brain.
3f 3. gp120-CD4 interaction of HIV isolates from the CNS.
28 4. gag assembly in macrophages.
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1e Lab personnel:
1c Wei Cao (Technician)
25 Maria Chen (Graduate Student)
29 Alexia Harrist (Graduate Student)
1f Tom Harvey (Technician)
2d Matthew Plassmeyer (Graduate Student)
2a Elena Ryzhova (Research Associate)
2d Samantha Soldan (Postdoctoral Fellow)
23 Karen Stachelek (Technician)
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Description of Research Expertise
2b Research Interests3d HIV Neuropathogenesis, Neurovirulence, Bunyaviruses.
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4a Key words: HIV, microglia, neuropathogenesis, HIV Dementia.
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26 Description of Research
4c Our laboratory studies viral neurotropism, in two groups of viruses.
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29e Human immunodeficiency virus causes a dementia, in addition to its primary effects on the immune system. The projects in the laboratory are primarily oriented to defining the pathophysiology of this HIV Dementia, which may be mediated by either direct infection of astrocytes, neurons and oligodendrocytes, or more likely, by indirect effects from infection of microglia. Current projects involve the analysis of CD4 dependence of HIV strains and the potential for development of neutralization-resistant strains in the CNS environment. An SIV project will look at viral evolution within the CNS, including analysis of viral genomes at the level of single cells.
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180 La Crosse virus is a member of the Bunyaviridae, a large family of viruses that includes the hantaviruses, which cause hantavirus pulmonary syndrome. La Crosse is a common cause of pediatric encephalitis. Current La Crosse projects look at the regions of the viral glycoproteins responsible for entry into muscle cells, and at the use of siRNA to inhibit La Crosse infections.
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2e Rotation Projects for 2006-2007
3c 1. Role of NSs in inhibition of siRNA in La Crosse.
49 2. Analysis of SIV gp120 sequences recovered from CSF and brain.
3f 3. gp120-CD4 interaction of HIV isolates from the CNS.
28 4. gag assembly in macrophages.
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1e Lab personnel:
1c Wei Cao (Technician)
25 Maria Chen (Graduate Student)
29 Alexia Harrist (Graduate Student)
1f Tom Harvey (Technician)
2d Matthew Plassmeyer (Graduate Student)
2a Elena Ryzhova (Research Associate)
2d Samantha Soldan (Postdoctoral Fellow)
23 Karen Stachelek (Technician)
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175 Ryzhova, E.V., Whitbeck,C., Canziani, G., Westmoreland, S.V., Cohen, G., Eisenberg, R., Lackner, A.A., and González-Scarano, F. : Rapid Progression to SAIDS Can Be Accompanied by Selection of CD4-independent gp120 Variants with Impaired Ability to Bind CD4 Journal of Virology 76: 7903-7090, 2002.
154 Chen, M.F., Westmoreland, S., Ryzhova, E.V., Martín-García, J., Soldan, S.S., Lackner, A., and González-Scarano F. : Simian Immunodeficiency Virus Envelope Compartmentalizes in Brain Regions Independent of Neuropathology. Journal of Neurovirology 12: 73-89, 2006.
14c Van der Meer, P., Goldberg, S.H., Fung, K.M., Sharer, L.R., González-Scarano, F., and Lavi, E. : Expression Pattern of CXCR3, CXCR4, and CCR3 Chemokine Receptors in the Developing Brain Journal of Neuropathology and Experimental Neurology 60: 25-32, 2001.
109 Soldan, S.S., Plassmeyer, M.L., Matukonis, M.K., and González-Scarano, F.: La Crosse virus Non-structural Protein NSs Counteracts the Effects of siRNA. Journal of Virology 79: 234-244, 2005.
c4 González-Scarano, F. and Martín-García, J.: The Neuropathogenesis of AIDS. Nature Reviews in Immunology 5: 69-81, 2005.
15a Martín-García J., Cao W., Varela-Rohena A., Plassmeyer M.L., and González-Scarano F.: HIV-1 Tropism for the Central Nervous System: Brain-Derived Envelope Glycoproteins with Lower CD4-Dependence and Reduced Sensitivity to a Fusion Inhibitor. Virology 346: 169-179, 2006.
133 Plassmeyer, M.L., Soldan, S.S., Stachelek, K.M., Roth, S.M., Martín-García, J., and González-Scarano, F: Mutagenesis of the La Crosse virus glycoproteins supports a role for Gc (1066-1087) as the Fusion peptide. Virology 358, 2007.
124 Ryzhova, E., Aye, P., Harvey, T., Cao, W., Lackner, A., ad González-Scarano, F: Intrathecal humoral responses are inversely associated with the frequency of SIV M-tropic variants in the CNS. Journal of Virology 2009.
138 Harrist, A.V., Ryzhova, E.V., Harvey, T., and González-Scarano, F. : Anx2 interacts with HIV-1 Gag at phosphatidylinositol (4,5) bisphosphate-containing lipid rafts and increases viral production in 293T cells. PLoSONE 4(3): e5020, 2009.
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Selected Publications
155 Ryzhova E.V., Crino P, Shawver L., Westmoreland S.V., Lackner A.A., and González-Scarano. F. : Simian Immunodeficiency Virus Encephalitis: Analysis of envelope sequences from individual brain multinucleated giant cells and tissue samples. Virology 297: 57-67, 2002.175 Ryzhova, E.V., Whitbeck,C., Canziani, G., Westmoreland, S.V., Cohen, G., Eisenberg, R., Lackner, A.A., and González-Scarano, F. : Rapid Progression to SAIDS Can Be Accompanied by Selection of CD4-independent gp120 Variants with Impaired Ability to Bind CD4 Journal of Virology 76: 7903-7090, 2002.
154 Chen, M.F., Westmoreland, S., Ryzhova, E.V., Martín-García, J., Soldan, S.S., Lackner, A., and González-Scarano F. : Simian Immunodeficiency Virus Envelope Compartmentalizes in Brain Regions Independent of Neuropathology. Journal of Neurovirology 12: 73-89, 2006.
14c Van der Meer, P., Goldberg, S.H., Fung, K.M., Sharer, L.R., González-Scarano, F., and Lavi, E. : Expression Pattern of CXCR3, CXCR4, and CCR3 Chemokine Receptors in the Developing Brain Journal of Neuropathology and Experimental Neurology 60: 25-32, 2001.
109 Soldan, S.S., Plassmeyer, M.L., Matukonis, M.K., and González-Scarano, F.: La Crosse virus Non-structural Protein NSs Counteracts the Effects of siRNA. Journal of Virology 79: 234-244, 2005.
c4 González-Scarano, F. and Martín-García, J.: The Neuropathogenesis of AIDS. Nature Reviews in Immunology 5: 69-81, 2005.
15a Martín-García J., Cao W., Varela-Rohena A., Plassmeyer M.L., and González-Scarano F.: HIV-1 Tropism for the Central Nervous System: Brain-Derived Envelope Glycoproteins with Lower CD4-Dependence and Reduced Sensitivity to a Fusion Inhibitor. Virology 346: 169-179, 2006.
133 Plassmeyer, M.L., Soldan, S.S., Stachelek, K.M., Roth, S.M., Martín-García, J., and González-Scarano, F: Mutagenesis of the La Crosse virus glycoproteins supports a role for Gc (1066-1087) as the Fusion peptide. Virology 358, 2007.
124 Ryzhova, E., Aye, P., Harvey, T., Cao, W., Lackner, A., ad González-Scarano, F: Intrathecal humoral responses are inversely associated with the frequency of SIV M-tropic variants in the CNS. Journal of Virology 2009.
138 Harrist, A.V., Ryzhova, E.V., Harvey, T., and González-Scarano, F. : Anx2 interacts with HIV-1 Gag at phosphatidylinositol (4,5) bisphosphate-containing lipid rafts and increases viral production in 293T cells. PLoSONE 4(3): e5020, 2009.
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