Akiva S. Cohen, PhD
The Children's Hospital of Philadelphia
34th Street and Civic Center Boulevard
Abramson Research Center, Room 816-H
Philadelphia, PA 19104-4399
Fax: (267) 426-5165
University of Maryland College Park, 1985.
University of Maryland College Park, 1989.
University of Maryland School of Medicine, 1994.
Description of Research ExpertiseKEY WORDS:
Head Injury, Hippocampus, Cognitive Impairment
Injury-induced altered brain excitability, circuit rearrangement and synaptic function
Intracellular and extracellular recording, whole-cell patch-clamp recording, immunocytochemistry, biochemistry and calcium fluorescence. Visualized and blind in vitro recording techniques. Electrophysiologic recording in neuronal cultures as well as cell lines and conditioned fear response behavior.
Our principal research interest is focused on the fundamental cellular and molecular mechanisms that underlie cognitive impairments associated with traumatic brain injury. We are primarily concerned with alterations in neuronal excitability in the limbic system of the brain. This system has been shown to play a primary role in higher cognitive function e.g. learning and memory and is damaged in traumatic brain injury. We incorporate a variety of techniques to understand the nature and functional consequences of injury-induced alterations.
Our studies begin with conditioned fear response behavior to assess cognitive impairments and extracellular recording to evaluate injured hippocampal function. Unbiased stereology is then used to quantify the degree of cell death. Excitatory and inhibitory synaptic recording is utilized to further determine the function of surviving neurons. Immunocytochemical and biochemical techniques are used to examine specific proteins that have been altered by injury and may be underlying synaptic and/or circuit dysfunction. The combination of these methodologies should help elucidate putative mechanisms causing injury-induced cognitive deficits. A better understanding of these injury-induced alterations will provide insight for directing the development of potential therapies that would ameliorate cognitive dysfunction in traumatic brain injured patients.
Selected PublicationsBrian R. White; Jonah A. Padawer-Curry; Akiva S. Cohen; Daniel J. Licht; Arjun G. Yodh: Brain Segmentation, Spatial Censoring, and Averaging Techniques for Optical Functional Connectivity Imaging in Mice. Under review at Biomedical Optics Express 2019.
Kaitlin A. Folweiler, Guoxiang Xiong, Hannah E. Metheny, Gabriel Nah, Akiva S. Cohen: Traumatic brain injury diminishes feedforward activation of parvalbumin-expressing interneurons in the dentate gyrus. Under review at eNeuro 2019.
Rebecca Ahrens-Nicklas, Luis Tecedor, Arron Hall, Elena Lysenko, Akiva Cohen, Beverly L. Davidson, Eric Marsh. : Altered neuronal circuit dynamics precede storage material accumulation or neurodegeneration in a lysosomal storage disorder. Under review at Journal of Clinical Investigation 2019.
Paterno Rosalia, Metheny Hannah, Cohen Akiva S: Memory deficit in an object location task after mild TBI is associated with impaired early object exploration and both are restored by branched chain amino acid dietary therapy. Journal of neurotrauma May 2018.
Elliott Jonathan E, De Luche Samuel E, Churchill Madeline J, Moore Cindy, Cohen Akiva S, Meshul Charles K, Lim Miranda M: Dietary therapy restores glutamatergic input to orexin/hypocretin neurons after traumatic brain injury in mice. Sleep 41(3), Mar 2018.
Folweiler Kaitlin, Samuel Sandy, Metheny Hannah, Cohen Akiva S: Diminished dentate gyrus filtering of cortical input leads to enhanced area CA3 excitability after mild traumatic brain injury. Journal of neurotrauma Jan 2018.
Xiong Guoxiang, Metheny Hannah, Johnson Brian N, Cohen Akiva S: A Comparison of Different Slicing Planes in Preservation of Major Hippocampal Pathway Fibers in the Mouse. Frontiers in neuroanatomy 11: 107, Nov 2017.
Wolf John A, Johnson Brian N, Johnson Victoria E, Putt Mary E, Browne Kevin D, Mietus Constance J, Brown Daniel P, Wofford Kathryn L, Smith Douglas H, Grady M Sean, Cohen Akiva S, Cullen D Kacy: Concussion Induces Hippocampal Circuitry Disruption in Swine. Journal of neurotrauma 34(14): 2303-2314, Jul 2017.
Yuan Feng, Xiong Guoxiang, Cohen Noam A, Cohen Akiva S: Optimized Protocol of Methanol Treatment for Immunofluorescent Staining in Fixed Brain Slices. Applied immunohistochemistry & molecular morphology : AIMM 25(3): 221-224, Mar 2017.
Paterno Rosalia, Metheny Hannah, Xiong Guoxiang, Elkind Jaclynn, Cohen Akiva S: Mild Traumatic Brain Injury Decreases Broadband Power in Area CA1. Journal of neurotrauma 33(17): 1645-9, Sep 2016.