A. Clementina Mesaros, PhD

faculty photo
Research Assistant Professor of Pharmacology
Department: Pharmacology

Contact information
421 Curie Blvd.
BRB II/III, room 856
Philadelphia, PA 19104
Office: 2154592116
Fax: 2155739889
Lab: 2155739878
B.Sc. (Chemistry and biochemistry)
Babes-Bolyai University, Cluj-Napoca, Romania, 1996.
MS. ( Chemistry of heterocyclic compunds)
Babes-Bolyai University, Cluj-Napoca, Romania, 1997.
Ph.D. (Bio-organic chemistry )
Department of Chemistry, Case Western Reserve University, Cleveland, OH. , 2005.
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Description of Research Expertise

My expertise is in developing assays for metabolites involved in cellular oxidative stress and flux analysis of metabolites within intermediate metabolic pathways using stable-isotope dilution liquid chromatography mass spectrometry (LC-MS).

Some of the routine assays include quantification of biomarkers of oxidative stress including isoprostanes, 8-oxo-dGuo and mechanisms of bioactive lipid formation and signaling pathways related to environmental exposures. As opposed to simply assessing exposure based on direct quantification of the exposure itself, this approach attempts to make use of downstream consequences of exposures.

I am currently working to discover new biomarkers of response to asbestos exposure in human population. Using an untargeted LC-MS metabolomics approach it was possible to identify several lipids that are dysregulated in serum from asbestos exposed subjects when compared with healthy controls.

My new research will look at systemic side-effects of inhaled corticosteroids in asthma. Rapid systemic metabolism of ICS is desired to reduce systemic effects, however, local metabolism may also affect efficacy. Limited information is available on the enzymes involved in the metabolism of natural glucocorticoids and ICS. The systemic and local metabolism of cortisol are different for hepatic inactivation or lung inactivation. This research can shed light into the importance of different pathways in asthma treatment.

Description of Other Expertise

Day-to-day operation of a large mass spectrometry facility core, including training and supervising the technical support staff and graduate and postgraduate students and ensuring adequate quality control.

Selected Publications

Lili Guo, Andrew J. Worth, Clementina Mesaros, Nathaniel W. Snyder, Jerry D. Glickson, and Ian A. Blair: Diisopropylethylamine/hexafluoroisopropanol-mediated ion-pairing UHPLC-MS for phosphate and carboxylate metabolite analysis: utility for studying cellular metabolism. Rapid Communications in Mass Spectrometry 30(16), August 2016.

Zang T1, Tamae D1, Mesaros C2, Wang Q2, Huang M1, Blair IA3, Penning TM4.: Simultaneous Quantitation of Nine Hydroxy-Androgens and Their Conjugates in Human Serum by Stable Isotope Dilution Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry. J Steroid Biochem Mol Biol S0960(1): 0760(16)30216-3, August 2016.

Mazaleuskaya Liudmila L, Lawson John A, Li Xuanwen, Grant Gregory, Mesaros Clementina, Grosser Tilo, Blair Ian A, Ricciotti Emanuela, FitzGerald Garret A: A broad-spectrum lipidomics screen of antiinflammatory drug combinations in human blood. JCI insight 1(12), Aug 2016.

Huang Meng, Mesaros Clementina, Zhang Suhong, Blair Ian A, Penning Trevor M: Potential Metabolic Activation of a Representative C2-Alkylated Polycyclic Aromatic Hydrocarbon 6-Ethylchrysene Associated with the Deepwater Horizon Oil Spill in Human Hepatoma (HepG2) Cells. Chemical research in toxicology 29(6): 991-1002, Jun 2016.

Vázquez-Medina José Pablo, Dodia Chandra, Weng Liwei, Mesaros Clementina, Blair Ian A, Feinstein Sheldon I, Chatterjee Shampa, Fisher Aron B: The phospholipase A2 activity of peroxiredoxin 6 modulates NADPH oxidase 2 activation via lysophosphatidic acid receptor signaling in the pulmonary endothelium and alveolar macrophages. FASEB journal : official publication of the Federation of American Societies for Experimental Biology Page: in press, May 2016.

Worth Andrew J, Marchione Dylan M, Parry Robert C, Wang Qingqing, Gillespie Kevin P, Saillant Noelle N, Sims Carrie, Mesaros Clementina, Snyder Nathaniel W, Blair Ian A: LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism. Journal of visualized experiments : JoVE(110), in press, April 2016.

Bedi Kenneth C, Snyder Nathaniel W, Brandimarto Jeffrey, Aziz Moez, Mesaros Clementina, Worth Andrew J, Wang Linda L, Javaheri Ali, Blair Ian A, Margulies Kenneth B, Rame J Eduardo: Evidence for Intramyocardial Disruption of Lipid Metabolism and Increased Myocardial Ketone Utilization in Advanced Human Heart Failure. Circulation 133(8): 706-16, Feb 2016.

Wang Qingqing, Mesaros Clementina, Blair Ian A: Ultra-high sensitivity analysis of estrogens for special populations in serum and plasma by liquid chromatography-mass spectrometry: Assay considerations and suggested practices. The Journal of steroid biochemistry and molecular biology Page: in press, Jan 2016.

Hergott Christopher B, Roche Aoife M, Naidu Nikhil A, Mesaros Clementina, Blair Ian A, Weiser Jeffrey N: Bacterial exploitation of phosphorylcholine mimicry suppresses inflammation to promote airway infection. The Journal of clinical investigation 125(10): 3878-90, Oct 2015.

Huang Meng, Zhang Li, Mesaros Clementina, Hackfeld Linda C, Hodge Richard P, Blair Ian A, Penning Trevor M: Metabolism of an Alkylated Polycyclic Aromatic Hydrocarbon 5-Methylchrysene in Human Hepatoma (HepG2) Cells. Chemical research in toxicology 28(10): 2045-58, Oct 2015.

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Last updated: 08/30/2016
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