David R. Manning, Ph.D.

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Emeritus Professor of Pharmacology
Academic Coordinator for Graduate Education, Department of Pharmacology, University of Pennsylvania School of Medicine
Department: Pharmacology

Contact information
Department of Systems Pharmacology and Translational Therapeutics
B.A. (Biology)
University of California at San Diego, 1975.
Ph.D. (Physiology/Pharmacology)
University of California at San Diego, 1980.
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Description of Research Expertise

Summary of Activities

Dr. Manning is best known for his work on heterotrimeric G proteins, molecular entities that translate the binding of hormones and neurotransmitters at receptors on cell surfaces into intracellular signals to which the cell responds. Particular interests of Dr. Manning are the development of antibodies to G proteins for use as tools to evaluate how these entities are distributed among cells and the extent to which they are subject to co- and posttranslational modifications; the development of assays to evaluate the selectivity of coupling between receptors and G proteins; the functions of particular G proteins, especially in platelets and neurons; and the extent to which morphogens involved in embryonic patterning and stem cell regeneration employ G proteins.

Dr. Manning was an Established Investigator for the American Heart Association. His research was funded throughout his tenure at Penn through grants from this and other private organizations and the National Institutes of Health.

Dr. Manning’s editorial positions included The Journal of Pharmacology and Experimental Therapeutics, Molecular Pharmacology, and The Journal of Biological Chemistry. He was a member and chair of the National Institutes of Health Cellular Biology and Physiology-2 Study Section and chair of the Division of Molecular Pharmacology for the American Society for Pharmacology and Experimental Therapeutics.

Dr. Manning established the undergraduate course ‘Principals of Drug Action’ within the Department of Biology. He contributed as a lecturer and often course director to a variety of other courses at both graduate and professional (medical) levels. His awards for teaching include the Dean’s Award for Excellence in Graduate Student Training, the Christian R. and Mary F. Lindback Award for Distinguished Teaching, the inaugural Annual Faculty Award from the Graduate Group in Pharmacological Sciences, and the Excellence in Teaching Award from the Department of Biology.

Dr. Manning’s administrative appointments included the chairmanship of the Graduate Group in Pharmacological Sciences, Academic Coordinator for Graduate Education for the Department of Pharmacology, Director of Undergraduate Initiatives for the Clinical and Translation Research Award, and Director of Training Support and Career Development for Biomedical Graduate Studies.

Selected Publications

Guo, X., N.A. Riobo-Del Galdo, E.J. Kim, G.R. Grant, and D.R. Manning: Overlap in signaling between Smoothened and the alpha subunit of the heterotrimeric G protein G13. PLOS ONE 2018 Notes: https://doi.org/10.1371/journal.pone0197442.

Shen, F, L Cheng, AE Douglas, NA Riobo, and DR Manning: Smoothened is a fully competent activator of the heterotrimeric G protein G(i). Mol Pharmacol 83: 691, 2013.

Polizio, A.H., Chinchilla, P., Chen, X., Kim, S., Manning, D.R., Riobo, N.A.: Heterotrimeric Gi proteins link Hedgehog signaling to activation of Rho small GTPases to promote fibroblast migration. J Biol Chem 286: 19589-19596, 2011.

Douglas, A.E., Heim, J.A., Shen, F., Amada, L.L., Riobo, N.A., Fernandez-Zapico, M.E., Manning, D.R.: The alpha subunit of the G protein G13 regulates the activity of one or more Gli transcription factors independently of Smoothened. J Biol Chem 286: 30714, 2011.

Crouthamel M, Abankwa D, Zhang L, Dilizio C, Manning DR, Hancock JF, Wedegaertner PB: An N-terminal polybasic motif of G{alpha}q is required for signaling and influences membrane nanodomain distribution Mol Pharmacol Page: In Press [Epub ahead of print] July 27 2010.

Zhang L, Brass LF and Manning DR: The Gq and G12 families of heterotrimeric G proteins report functional selectivity. Mol Pharmacol 75: 235-241, 2009.

Riobo NA, Saucy B, Dilizio C, Manning DR: Activation of heterotrimeric G proteins by Smoothened. Proc Natl Acad Sci USA 103: 12607-12612, 2006.

Zhang L, DiLizio C, Kim D, Smyth EM, Manning DR: The G12 family of G proteins as a reporter of thromboxane A2 receptor activity. Molec Pharmacol 69: 1433-1440, 2006.

Windh RT and Manning DR: Analysis of G protein activation in Sf9 and mammalian cells by agonist-promoted [35S]GTP gamma S binding. Methods in Enzymology 344: 3-14, 2002.

Windh RT and Manning DR: Expression of G protein-coupled receptors and G proteins in Sf9 cells: Analysis of coupling by radioligand binding. Methods in Enzymology 343: 417-429, 2002.

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Last updated: 08/19/2022
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