David W. Speicher, Ph.D.
Wistar Institute Professor of Biochemistry and Biophysics
Member, Institute for Medicine & Engineering, University of Pennsylvania
Member, Graduate Group in Biochemistry & Molecular Biophysics, University of Pennsylvania
Member, Graduate Group in Genomics & Computational Biology, University of Pennsylvania
Department: Biochemistry and Biophysics
Contact information
The Wistar Institute
3601 Spruce Street, Room 272A
Philadelphia, PA 19104
3601 Spruce Street, Room 272A
Philadelphia, PA 19104
Office: 215-898-3972
Fax: 215-495-6915
Fax: 215-495-6915
Email:
speicher@wistar.org
speicher@wistar.org
Links
Proteomics Core Facility, The Wistar Institute
David W. Speicher, Ph.D. Laboratory, The Wistar Institute
Proteomics Core Facility, The Wistar Institute
David W. Speicher, Ph.D. Laboratory, The Wistar Institute
Education:
B.S. (Biochemistry)
Pennsylvania State University, 1972.
Ph.D. (Biochemistry)
Pennsylvania State University, 1977.
Permanent linkB.S. (Biochemistry)
Pennsylvania State University, 1972.
Ph.D. (Biochemistry)
Pennsylvania State University, 1977.
Description of Research Expertise
Key Words: cancer biomarkers; tumor progression; proteomics; protein assembly and thermodynamics; membrane skeleton; mass spectrometry; systems biologyResearch Interests:
* Chemical crosslinking
* Modeling and verification of protein complexes using chemical crosslinking
* Proteome and systems biology analysis of human cancer metastasis
* Proteomic analysis of plasma biomarkers and biosignatures of cancer
* Biomarkers of ectopic pregnancy
* Proteome analysis of oxidative stress and role of peroxiredoxin 6 in acute lung injury
* Macromolecular interaction mechanisms and thermodynamics in complex protein systems
* Structure, function, assembly and regulation of membrane skeletal proteins
Selected Publications
Wang Huan, Chang-Wong Tony, Tang Hsin-Yao, Speicher David W: Comparison of Extensive Protein Fractionation and Repetitive LC-MS/MS Analyses on Depth of Analysis for Complex Proteomes. Journal of proteome research 9(2): 1032-40, Dec 2009.Gaetani Massimiliano, Mootien Sara, Harper Sandra, Gallagher Patrick G, Speicher David W: Structural and functional effects of hereditary hemolytic anemia-associated point mutations in the alpha spectrin tetramer site. Blood 111(12): 5712-20, Jun 2008.
Han Mee-Jung, Herlyn Meenhard, Fisher Aron B, Speicher David W: Microscale solution IEF combined with 2-D DIGE substantially enhances analysis depth of complex proteomes such as mammalian cell and tissue extracts. Electrophoresis 29(3): 695-705, Feb 2008.
Li Donghai, Tang Hsin-Yao, Speicher David W: A structural model of the erythrocyte spectrin heterodimer initiation site determined using homology modeling and chemical cross-linking. The Journal of biological chemistry 283(3): 1553-62, Jan 2008.
Li Donghai, Harper Sandra, Speicher David W: Initiation and propagation of spectrin heterodimer assembly involves distinct energetic processes. Biochemistry 46(37): 10585-94, Sep 2007.
Johnson Colin P, Tang Hsin-Yao, Carag Christine, Speicher David W, Discher Dennis E: Forced unfolding of proteins within cells. Science (New York, N.Y.) 317(5838): 663-6, Aug 2007.
Liu Qin, Tan Glenn, Levenkova Natasha, Li Tiansen, Pugh Edward N, Rux John J, Speicher David W, Pierce Eric A: The proteome of the mouse photoreceptor sensory cilium complex. Molecular & cellular proteomics : MCP 6(8): 1299-317, Aug 2007.
Hoffman Seth A, Joo Won-A, Echan Lynn A, Speicher David W: Higher dimensional (Hi-D) separation strategies dramatically improve the potential for cancer biomarker detection in serum and plasma. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 849(1-2): 43-52, Apr 2007.
Tang Hsin-Yao, Ali-Khan Nadeem, Echan Lynn A, Levenkova Natasha, Rux John J, Speicher David W: A novel four-dimensional strategy combining protein and peptide separation methods enables detection of low-abundance proteins in human plasma and serum proteomes. Proteomics 5(13): 3329-42, Aug 2005.
Tang Hsin-Yao, Speicher David W: In vivo phosphorylation of human erythrocyte spectrin occurs in a sequential manner. Biochemistry 43(14): 4251-62, Apr 2004.