Matthew C Good, Ph.D.

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Assistant Professor of Cell and Developmental Biology
Department: Cell and Developmental Biology
Graduate Group Affiliations

Contact information
1151 BRB II/III
421 Curie Boulevard
Philadelphia, PA 19104
Office: 215-573-1099
Lab: 215-573-6805
Education:
BA (Biochemistry)
University of California, Berkeley, 2003.
PhD (Biochemistry)
University of California, San Francisco, 2010.
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Description of Research Expertise

RESEARCH INTERESTS
* Adaptability of intracellular structures to variation in cell size and shape
* Temporal and spatial regulation of zygotic genome activation
* Self-assembly of intrinsically disordered proteins
* Phase separation and membraneless organelles
* Subcellular organization of intracellular signaling pathways
* Synthetic cells and protocells, synthetic organelles
* Optochemical protein dimerization

KEY WORDS
cell size, intracellular size scaling, size regulation, embryogenesis, zygotic genome activation, transcriptional imaging, Xenopus, zebrafish, optochemical dimerization, split enzymes, cell-free systems, encapsulation, microfluidics, emulsions, synthetic cells, IDPs, membraneless organelles

DESCRIPTION OF RESEARCH

Our lab is interested: 1) cell size control and the downstream consequences of size-dysregulation, and 2) coordinated protein assembly into mesoscale structures, including membraneless organelles, the mitotic spindle and signaling complexes. To investigate the impacts of cell size on cell function, we characterize early embryo development, a period in which cells rapidly divide without growing. Additionally, we are investigating diseased cell states, such as cancer, in which proper cell size homeostasis is lost. To characterize principles that drive the self-assembly of proteins, we focus on scaling and positioning of the mitotic spindle within cleavage stage embryos and in vitro. Additionally we want to understand the properties of proteins that drive their self-assembly and phase separation into membraneless organelles.

We utilize a set of interdisciplinary approaches to address the fundamental questions in cell biology. This strategy requires that we adapt and build new tools, including imaging of transcription during embryo development; microfluidic approaches to encapsulate proteins and cell-free cytoplasmic mixtures inside cell-like compartments in vitro; optochemical dimerizers to control protein localization and enzymatic activity within synthetic cells. Our model systems include: zebrafish and Xenopus cleavage-stage embryos, mammalian cell culture, and synthetic cells. We leverage numerous biochemical approaches extoll the power of bottom-up reconstitution to recapitulate cellular processes ex vivo.

Selected Publications

Schuster B.S., Reed E.H., Parthasarathy R., Jahnke C.N., Caldwell R.M., Bermudez J.G., Ramage H., Good M.C.*, Hammer D.A.*: Controllable Protein Phase Separation and Modular Recruitment to Form Responsive, Membraneless Organelles. Nature Communications 9(2985), July 2018.

Caldwell R.M., Bermudez J.G., Thai D., Aonbangkhen C., Schuster B.S., Courtney T., Deiters A., Hammer D.A., Chenoweth D.M., Good M.C.*: Optochemical Control of Protein Localization and Activity within Cell-like Compartments. Biochemistry 57(18): 2590-2596, May 2018.

Bermudez J.G., Chen H., Einstein L.C., Good M.C.*: Probing the Biology of Cell Boundary Conditions Through Confinement of Xenopus Cell-Free Cytoplasmic Extracts. Genesis 55(1-2), Jan 2017.

Chen, H. and Good, M.C.: Size Regulation: Big Insights from Little Cells. Developmental Cell 37: 392-394, June 2016.

Good M.C.*: Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions In Synthetic Cell-like Compartments with Tunable Size. Methods in Molecular Biology 1413: 87-108, May 2016 Notes: 10.1007/978-1-4939-3542-0_7.

Good, M.C.: Turn up the Volume: Uncovering Nucleus Size Control Mechanisms. Developmental Cell 33: 496-497, June 2015.

Crowder, M.E., Strzelecka, M., Wilbur, J.D., Good, M.C., von Dassow, G., and Heald, R. : A Comparative Analysis of Spindle Morphometrics across Metazoans. Current Biology 25: 1542-1550, May 2015.

Good MC, Zalatan JG, Lim WA.: Scaffold proteins: hubs for controlling the flow of cellular information. Science 332(6030): 680-6, May 2011.

Good MC, Vahey MD, Skandarajah A, Fletcher DA, Heald R.: Cytoplasmic volume modulates spindle size during embryogenesis. Science 342(6160): 856-60, Nov 2013.

Good M, Tang G, Singleton J, Reményi A, Lim WA.: The Ste5 scaffold directs mating signaling by catalytically unlocking the Fus3 MAP kinase for activation. Cell 136(6): 1085-97, Mar 2009.

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Last updated: 08/23/2018
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