Matthew C Good, Ph.D.

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Assistant Professor of Cell and Developmental Biology
Department: Cell and Developmental Biology
Graduate Group Affiliations

Contact information
1151 BRB II/III
421 Curie Boulevard
Philadelphia, PA 19104
Office: 215-573-1099
Lab: 215-573-6805
Education:
BA (Biochemistry)
University of California, Berkeley, 2003.
PhD (Biochemistry)
University of California, San Francisco, 2010.
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Description of Research Expertise

RESEARCH INTERESTS
- Adaptability of intracellular structures to variation in cell size and shape
- Impacts of cell geometry on subcellular assembly and gene expression
- Spatial organization of intracellular signaling pathways;
- Extreme cell size and early embryo development

KEY WORDS
cell size, intracellular size scaling, cell-free systems, encapsulation, microfluidics, synthetic cells, Xenopus, embryogenesis, development

DESCRIPTION OF RESEARCH
The goal of the lab is to understand how cell size and shape regulate cellular function, and how cell and organelle size becomes misregulated during disease. We use the lens of early embryo development, in which cellular dimensions are rapidly reduced after fertilization, to explore scaling relationships between cells and intracellular structures (e.g. the mitotic spindle). To characterize mechanisms of size-regulation we create synthetic, cell-like compartments with tunable geometry, combining cell-free cytoplasmic extracts and microfluidic droplet- and vesicle-generating technologies. This approach, which brings together tools from cell biology, chemistry and bioengineering, allows us to isolate the effects of cell size on intracellular assembly and function, free from constraints of a developmental program. Our broad aims are to investigate cell size as a control parameter, uncover adaptions required for intracellular processes to function in cells with extreme geometries, and develop new technologies for reconstituting complex cellular behaviors ex vivo.

Selected Publications

Bermudez J.G., Chen H., Einstein L.C., Good M.C.: Probing the Biology of Cell Boundary Conditions Through Confinement of Xenopus Cell-Free Cytoplasmic Extracts. Genesis 55, Jan 2017.

Chen, H. and Good, M.C.: Size Regulation: Big Insights from Little Cells. Developmental Cell 37: 392-394, June 2016.

Good M.C.: Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions In Synthetic Cell-like Compartments with Tunable Size. Methods in Molecular Biology 1413: 87-108, May 2016.

Good, M.C.: Turn up the Volume: Uncovering Nucleus Size Control Mechanisms. Developmental Cell 33: 496-497, June 2015.

Crowder, M.E., Strzelecka, M., Wilbur, J.D., Good, M.C., von Dassow, G., and Heald, R. : A Comparative Analysis of Spindle Morphometrics across Metazoans. Current Biology 25: 1542-1550, May 2015.

Good MC, Vahey MD, Skandarajah A, Fletcher DA, Heald R.: Cytoplasmic volume modulates spindle size during embryogenesis. Science 342: 856-60, Nov 2013.

Good MC, Zalatan JG, Lim WA.: Scaffold proteins: hubs for controlling the flow of cellular information. Science 332: 680-6, May 2011.

Good M, Tang G, Singleton J, Reményi A, Lim WA.: The Ste5 scaffold directs mating signaling by catalytically unlocking the Fus3 MAP kinase for activation. Cell 136(6): 1085-97, Mar 2009.

King N, Westbrook MJ, Young SL, Kuo A, Abedin M, Chapman J, Fairclough S, Hellsten U, Isogai Y, Letunic I, Marr M, Pincus D, Putnam N, Rokas A, Wright KJ, Zuzow R, Dirks W, Good M, Goodstein D, Lemons D, Li W, Lyons JB, Morris A, Nichols S, Richter DJ, Salamov A, Sequencing JG, Bork P, Lim WA, Manning G, Miller WT, McGinnis W, Shapiro H, Tjian R, Grigoriev IV, Rokhsar D.: The genome of the choanoflagellate Monosiga brevicollis and the origin of metazoans. Nature 451(7180): 783-8, Feb 2008.

Bhattacharyya RP, Reményi A, Good MC, Bashor CJ, Falick AM, Lim WA.: The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway. Science 311(5762): 822-6, Feb 2006.

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Last updated: 12/04/2017
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