Holly Ramage, PhD

faculty photo
Adjunct Assistant Professor of Microbiology
Department: Microbiology

Contact information
473A Stemmler
3450 Hamilton Walk
Philadelphia, PA 19104
Office: 2158986857
B.S. (Molecular and Cellular Biology)
California State University, Fresno, 2000.
M.S. (Biology)
California State University, Fresno, 2003.
Ph.D. (Genetics)
University of California San Francisco, 2010.
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Description of Research Expertise

Research Interests: virus-host interactions, host co-factors of viral infection, protein-protein interactions, Flaviviridae viruses

Keywords: virus-host interactions, hepatitis C virus, West Nile virus, proteomics

Description of Research: My long-term research interests center on understanding the molecular mechanisms of viral-host interactions and the application of this information to develop new antiviral strategies. We have used mass spectrometry technologies to identify cellular factors that interact with Hepatitis C virus through a collaboration with Dr. Nevan Krogan. We comprehensively characterized HCV-host protein-protein interactions, and tested the functional requirements of these interactions using an RNAi screen. This led to the identification of 69 new host factors that physically interact with HCV and influence infection. This wealth of data serves as a platform for as I continue my work at the University of Pennsylvania. We are particularly interested in host factors that are involved in infection with related viruses and have generated a West Nile virus-host protein-protein interaction dataset to make these comparisons.

Selected Publications

Li M, Johnson JR, Truong B, Kim G, Weinbren N, Dittmar M, Shah PS, Von Dollen J, Newton BW, Jang GM, Krogan NJ, Cherry S, Ramage H: Comparative Flavivirus-Host Protein Interaction Mapping Reveals Mechanisms of Dengue and Zika Virus Pathogenesis. Nature Microbiology 2019.

Shah PS, Link N, Jang GM, Sharp PP, Zhu T, Swaney DL, Johnson JR, Von Dollen J, Ramage HR, Satkamp L, Newton B, Hüttenhain R, Petit MJ, Baum T, Everitt A, Laufman O, Tassetto M, Shales M, Stevenson E, Iglesias GN, Shokat L, Tripathi S, Balasubramaniam V, Webb LG, Aguirre S, Willsey AJ, Garcia-Sastre A, Pollard KS, Cherry S, Gamarnik AV, Marazzi I, Taunton J, Fernandez-Sesma A, Bellen HJ, Andino R, Krogan NJ.: Comparative Flavivirus-Host Protein Interaction Mapping Reveals Mechanisms of Dengue and Zika Virus Pathogenesis. Cell 2018.

Schuster BS, Reed EH, Parthasarathy R, Jahnke CN, Caldwell RM, Bermudez JG, Ramage HR, Good MC, Hammer DA: Controllable protein phase separation and modular recruitment to form responsive, membraneless organelles Nature Comm. 9(1): 2985-2989, 2018.

Zhang R, Miner JJ, Gorman MJ, Rausch K, Ramage HR, White JP, Zuiani A, Zhang P, Fernandez E, Zhang Q, Dowd KA, Pierson TC, Cherry S, Diamond MS: A CRISPR screen defines a signal peptide processing pathway required by flaviviruses. Nature 535(7610): 164-168, June 2016.

Ali I, Ramage HR, Boehm D, Dirk LMA, Sakane N, Hanada K, Pagans S, Kaehlcke K, Aull K, Weinberger L, Trievel R, Schnoelzer M, Kamada M, Houtz R, Ott M: The HIV-1 Tat protein is monomethylated at lysine-71 by the lysine methyltransferase KMT7. J. Biol. Chem. 291(31): 16240-16248, May 2016.

Ramage HR, Cherry S: Virus-host Interactions: From Unbiased Genetic Screens to Function. Ann. Rev. in Virol. 2(1): 497-524, November 2015.

Ramage HR, Kumar GR, Verschueren E, Johnson JR, Von Dollen J, Johnson T, Newton B, Shah P, Horner J, Krogan NJ, Ott M: A combined proteomics/genomics approach links hepatitis C virus infection with nonsense-mediated mRNA decay. Mol. Cell 57(2): 329-340, January 2015.

Camus G, Herker E, Modi AA, Haas JT, Ramage HR, Farese RV, Ott M: Diacylglycerol acyltransferase-1 localizes hepatitis C virus NS5A protein to lipid droplets and enhances NS5A interaction with the viral capsid core. J. Biol. Chem. 288(14): 9915-23, April 2013.

Ramage HR, Connolly LE, Cox JS: Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12): e1000767, December 2009.

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Last updated: 03/02/2020
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