Holly Ramage, PhD

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Research Assistant Professor of Microbiology
Department: Microbiology

Contact information
301C Johnson Pavilion
3610 Hamilton Walk
Philadelphia, PA 19104
Education:
B.S. (Molecular and Cellular Biology)
California State University, Fresno, 2000.
M.S. (Biology)
California State University, Fresno, 2003.
Ph.D. (Genetics)
University of California San Francisco, 2010.
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Description of Research Expertise

Research Interests: virus-host interactions, host co-factors of viral infection, protein-protein interactions, Flaviviridae viruses

Keywords: virus-host interactions, hepatitis C virus, West Nile virus, proteomics

Description of Research: My long-term research interests center on understanding the molecular mechanisms of viral-host interactions and the application of this information to develop new antiviral strategies. We have used mass spectrometry technologies to identify cellular factors that interact with Hepatitis C virus through a collaboration with Dr. Nevan Krogan. We comprehensively characterized HCV-host protein-protein interactions, and tested the functional requirements of these interactions using an RNAi screen. This led to the identification of 69 new host factors that physically interact with HCV and influence infection. This wealth of data serves as a platform for as I continue my work at the University of Pennsylvania. We are particularly interested in host factors that are involved in infection with related viruses and have generated a West Nile virus-host protein-protein interaction dataset to make these comparisons.

Selected Publications

Zhang, R., Miner, J.J., Gorman, M.J., Rausch, K., Ramage, H., White, J.P., Zuiani, A., Zhang, P., Fernandez, E., Zhang, Q., Dowd, K.A., Pierson, T.C., Cherry, S., Diamond, M.S.: A CRISPR screen defines a signal peptide processing pathway required by flaviviruses. Nature 535(7610): 164-168, June 2016.

Ali, I., Ramage, H., Boehm, D., Dirk, L.M.A., Sakane, N., Hanada, K., Pagans, S., Kaehlcke, K., Aull, K., Weinberger, L., Trievel, R., Schnoelzer, M., Kamada, M., Houtz, R., Ott, M.: The HIV-1 Tat protein is monomethylated at lysine-71 by the lysine methyltransferase KMT7. The Journal of Biological Chemistry 291(31): 16240-16248, May 2016.

Ramage H, Cherry S.: Virus-Host Interactions: From Unbiased Genetic Screens to Function. Annual Review of Virology 2(1): 497-524, November 2015.

Ramage, H.R., Cherry, S.: Virus-host Interactions: From Unbiased Genetic Screens to Function. Ann. Rev. in Virol. 2(1): 497-524, November 2015.

Ramage, H.R., Kumar, G.R., Verschueren, E., Johnson, J.R., Von Dollen, J., Johnson, T., Newton, B., Shah, P., Horner, J., Krogan N.J., Ott, M.: A combined proteomics/genomics approach links hepatitis C virus infection with nonsense-mediated mRNA decay. Molecular Cell 57(2): 329-40, January 2015.

Camus G., Herker E., Modi A.A., Haas J.T., Ramage H.R., Farese R.V., Ott M.: Diacylglycerol acyltransferase-1 localizes hepatitis C virus NS5A protein to lipid droplets and enhances NS5A interaction with the viral capsid core. The Journal of Biological Chemistry 288(14): 9915-23, April 2013.

Ramage H.R., Connolly L.E., Cox J.S.: Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12): e1000767, December 2009.

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Last updated: 11/10/2017
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