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Melike Lakadamyali, Ph.D.

Melike Lakadamyali, Ph.D.

faculty photo
Assistant Professor of Physiology
Department: Physiology

Contact information
University of Pennsylvania, Perelman School of Medicine, Department of Physiology
415 Curie Blvd
764 Clinical Research Building
Philadelphia, PA 19104
Office: 2157465150
Fax: 2157465150
Lab: 2157465150
Education:
BSc (Physics)
University of Texas, Austin, 2001.
PhD (Physics)
Harvard University, 2006.
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Description of Research Expertise

My main interest is to study biology at the level of its macromolecular machines and to gain a quantitative biophysical understanding of how these machines drive important cell biological processes. Since new tools enable new biology, I also develop advanced microscopy methods that aim to overcome the limitations of current methods and help us visualize the macromolecular machineries of the cell in action with high spatiotemporal resolution. Specifically, I am interested in the molecular machinery involved in two fundamental biological processes: transport machinery that drives intracellular trafficking of vesicles and transcriptional machinery that drives gene expression. At the heart of and common to both biological problems is the interaction of multiple proteins with each other and with other proteins to form functional macromolecular nanoscopic complexes. The spatial and temporal organization of these interactions is tightly regulated and the failure to form these macromolecular complexes in the right place and at the right time can have catastrophic consequences. Indeed, protein copy number, protein clustering and protein mobility are all crucial parameters in intracellular transport and transcription; and failure to properly regulate them can lead to the difference between healthy function and disease.
Over the recent years, my group has been pioneering major developments in the field of super-resolution microscopy (Balint et al, PNAS 2013, Durisic et al Nature Methods 2014, Tam et al PLoS One 2014 (a), Tam et al PLoS One 2014 (b)). These methods have enabled us to gain novel insights into the transport of vesicles along their cytoskeletal tracks (Balint et al, PNAS, 2013) and the spatial organization of nucleosomes along the chromatin fiber (Ricci et al, Cell). Importantly, we have taken a highly quantitative biophysical approach in studying these biological processes, going beyond qualitative descriptions towards precise quantitative models. For example, we have made important strides in quantifying the stoichiometry of macromolecular assemblies such as nucleosomes at nanoscale resolution (Durisic et al, J. Neuroscience 2012, Durisic et al Nature Methods, 2014, Ricci et al Cell, 2015).

Selected Publications

Zanacchi Francesca Cella, Manzo Carlo, Alvarez Angel S, Derr Nathan D, Garcia-Parajo Maria F, Lakadamyali Melike: A DNA origami platform for quantifying protein copy number in super-resolution. Nature methods 14(8): 789-792, Aug 2017.

Kieffer-Kwon Kyong-Rim, Nimura Keisuke, Rao Suhas S P, Xu Jianliang, Jung Seolkyoung, Pekowska Aleksandra, Dose Marei, Stevens Evan, Mathe Ewy, Dong Peng, Huang Su-Chen, Ricci Maria Aurelia, Baranello Laura, Zheng Ying, Ardori Francesco Tomassoni, Resch Wolfgang, Stavreva Diana, Nelson Steevenson, McAndrew Michael, Casellas Adriel, Finn Elizabeth, Gregory Charles, St Hilaire Brian Glenn, Johnson Steven M, Dubois Wendy, Cosma Maria Pia, Batchelor Eric, Levens David, Phair Robert D, Misteli Tom, Tessarollo Lino, Hager Gordon, Lakadamyali Melike, Liu Zhe, Floer Monique, Shroff Hari, Aiden Erez Lieberman, Casellas Rafael: Myc Regulates Chromatin Decompaction and Nuclear Architecture during B Cell Activation. Molecular cell Jul 2017.

Verdeny-Vilanova Ione, Wehnekamp Fabian, Mohan Nitin, Sandoval Álvarez Ángel, Borbely Joseph Steven, Otterstrom Jason John, Lamb Don C, Lakadamyali Melike: 3D motion of vesicles along microtubules helps them to circumvent obstacles in cells. Journal of cell science 130(11): 1904-1916, Jun 2017.

Gaitán-Peñas Héctor, Gradogna Antonella, Laparra-Cuervo Lara, Solsona Carles, Fernández-Dueñas Victor, Barrallo-Gimeno Alejandro, Ciruela Francisco, Lakadamyali Melike, Pusch Michael, Estévez Raúl: Investigation of LRRC8-Mediated Volume-Regulated Anion Currents in Xenopus Oocytes. Biophysical journal 111(7): 1429-1443, Oct 2016.

Mannelli Ilaria, Reigada Ramon, Suárez Irina, Janner Davide, Carrilero Albert, Mazumder Prantik, Sagués Francesc, Pruneri Valerio, Lakadamyali Melike: Functionalized Surfaces with Tailored Wettability Determine Influenza A Infectivity. ACS applied materials & interfaces 8(24): 15058-66, Jun 2016.

Amit Ido, Baker David, Barker Roger, Berger Bonnie, Bertozzi Carolyn, Bhatia Sangeeta, Biffi Alessandra, Demichelis Francesca, Doudna Jennifer, Dowdy Steven F, Endy Drew, Helmstaedter Moritz, Junca Howard, June Carl, Kamb Sasha, Khvorova Anastasia, Kim Dae-Hyeong, Kim Jin-Soo, Krishnan Yamuna, Lakadamyali Melike, Lappalainen Tuuli, Lewin Sharon, Liao James, Loman Nick, Lundberg Emma, Lynd Lee, Martin Cathie, Mellman Ira, Miyawaki Atsushi, Mummery Christine, Nelson Karen, Paz Jeanne, Peralta-Yahya Pamela, Picotti Paola, Polyak Kornelia, Prather Kristala, Qin Jun, Quake Stephen, Regev Aviv, Rogers John A, Shetty Reshma, Sommer Morten, Stevens Molly, Stolovitzky Gustavo, Takahashi Masayo, Tang Fuchou, Teichmann Sarah, Torres-Padilla Maria-Elena, Tripathi Leena, Vemula Praveen, Verdine Greg, Vollmer Frank, Wang Jun, Ying Jackie Y, Zhang Feng, Zhang Tian: Voices of biotech. Nature biotechnology 34(3): 270-5, Mar 2016.

Torreno-Pina Juan A, Manzo Carlo, Salio Mariolina, Aichinger Michael C, Oddone Anna, Lakadamyali Melike, Shepherd Dawn, Besra Gurdyal S, Cerundolo Vincenzo, Garcia-Parajo Maria F: The actin cytoskeleton modulates the activation of iNKT cells by segregating CD1d nanoclusters on antigen-presenting cells. Proceedings of the National Academy of Sciences of the United States of America 113(6): E772-81, Feb 2016.

Lakadamyali Melike, Cosma Maria Pia: Advanced microscopy methods for visualizing chromatin structure. FEBS letters 589(20 Pt A): 3023-30, Oct 2015.

Ricci Maria Aurelia, Manzo Carlo, García-Parajo María Filomena, Lakadamyali Melike, Cosma Maria Pia: Chromatin fibers are formed by heterogeneous groups of nucleosomes in vivo. Cell 160(6): 1145-58, Mar 2015.

Planagumà Jesús, Leypoldt Frank, Mannara Francesco, Gutiérrez-Cuesta Javier, Martín-García Elena, Aguilar Esther, Titulaer Maarten J, Petit-Pedrol Mar, Jain Ankit, Balice-Gordon Rita, Lakadamyali Melike, Graus Francesc, Maldonado Rafael, Dalmau Josep: Human N-methyl D-aspartate receptor antibodies alter memory and behaviour in mice. Brain : a journal of neurology 138(Pt 1): 94-109, Jan 2015.

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Last updated: 08/04/2017
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