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Yi-Wei Chang, Ph.D.
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Assistant Professor of Biochemistry and Biophysics
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Associate Director, Institute of Structural Biology
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Department: Biochemistry and Biophysics
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Graduate Group Affiliations
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Contact information
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Department of Biochemistry and Biophysics
23 Perelman School of Medicine
22 University of Pennsylvania
20 913B Stellar-Chance Labs
37 422 Curie Blvd.
Philadelphia, PA 19104-6059
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23 Perelman School of Medicine
22 University of Pennsylvania
20 913B Stellar-Chance Labs
37 422 Curie Blvd.
Philadelphia, PA 19104-6059
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Office: (215) 898-7789
40 Lab: (215) 898-1191
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40 Lab: (215) 898-1191
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Email:
ywc@pennmedicine.upenn.edu
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ywc@pennmedicine.upenn.edu
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Publications
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Links
b6 Search PubMed for articles
cb - 2022 FIB-Milling Sample Preparation for Cellular CryoET Award, The Arnold and Mabel Beckman Foundation
54 - Lab Website
da - 2019 ASPIRE Award, The Mark Foundation for Cancer Research
8e - 2022 Catalysts Fellow, The EMBO Journal
108 - 2019 Packard Fellowships in Science and Engineering, The David and Lucile Packard Foundation
a5 - 2025 Michael S. Brown New Investigator Research Award
110 - 2023 Investigators in the Pathogenesis of Infectious Disease, The Burroughs Wellcome Fund
57 - ORCID
87 - Google Scholar Citations
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b6 Search PubMed for articles
cb - 2022 FIB-Milling Sample Preparation for Cellular CryoET Award, The Arnold and Mabel Beckman Foundation
54 - Lab Website
da - 2019 ASPIRE Award, The Mark Foundation for Cancer Research
8e - 2022 Catalysts Fellow, The EMBO Journal
108 - 2019 Packard Fellowships in Science and Engineering, The David and Lucile Packard Foundation
a5 - 2025 Michael S. Brown New Investigator Research Award
110 - 2023 Investigators in the Pathogenesis of Infectious Disease, The Burroughs Wellcome Fund
57 - ORCID
87 - Google Scholar Citations
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Education:
21 9 B.S. 24 (Mechanical Engineering ) c
35 Chung Hua University, Taiwan, 2004.
21 a Ph.D. 20 (Structural Biology ) c
50 Academia Sinica & National Tsing Hua University, Taiwan, 2010.
21 a Cert. 58 (Research Mentor Training: Effective Communication and Aligning Expectations) c
50 Perelman School of Medicine, University of Pennsylvania, 2022.
21 a Cert. 3d (Research Mentor Training: Fostering Independence) c
50 Perelman School of Medicine, University of Pennsylvania, 2025.
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Permanent link21 9 B.S. 24 (Mechanical Engineering ) c
35 Chung Hua University, Taiwan, 2004.
21 a Ph.D. 20 (Structural Biology ) c
50 Academia Sinica & National Tsing Hua University, Taiwan, 2010.
21 a Cert. 58 (Research Mentor Training: Effective Communication and Aligning Expectations) c
50 Perelman School of Medicine, University of Pennsylvania, 2022.
21 a Cert. 3d (Research Mentor Training: Fostering Independence) c
50 Perelman School of Medicine, University of Pennsylvania, 2025.
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21 Cryo-electron microscopy;
21 Cryo-electron tomography;
32 Correlative light and electron microscopy;
19 Cellular imaging;
15 Microbiology;
20 Host-pathogen interaction
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Description of Research Expertise
24 Structural biology;21 Cryo-electron microscopy;
21 Cryo-electron tomography;
32 Correlative light and electron microscopy;
19 Cellular imaging;
15 Microbiology;
20 Host-pathogen interaction
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10f Kathryn Kixmoeller, Ekaterina V. Tarasovetc, Elie Mer, Yi-Wei Chang*, Ben E Black*: Centromeric chromatin clearings demarcate the site of kinetochore formation. Cell 188(5): 1280-1296, January 2025.
13d Benjamin C Creekmore†, Kathryn Kixmoeller†, Ben E Black, Edward B Lee*, Yi-Wei Chang*: Ultrastructure of human brain tissue vitrified from autopsy revealed by cryo-ET with cryo-plasma FIB milling. Nature Communications 15: 2660, March 2024.
1ca Nicolas Dos Santos Pacheco†, Albert Tell i Puig†, Amandine Guérin†, Matthew Martinez, Bohumil Maco, Nicolò Tosetti, Estefanía Delgado-Betancourt, Matteo Lunghi, Boris Striepen, Yi-Wei Chang*, Dominique Soldati-Favre*: Sustained rhoptry docking and discharge requires Toxoplasma gondii intraconoidal microtubule-associated proteins. Nature Communications 15: 379, January 2024.
1a5 Matthew Martinez†, Shrawan Kumar Mageswaran†, Amandine Guérin, William David Chen, Cameron Thompson, Sarbin Chavin, Dominique Soldati-Favre, Boris Striepen, Yi-Wei Chang*: Origin and arrangement of actin filaments for gliding motility in apicomplexan parasites revealed by cryo-electron tomography. Nature Communications 14: 4800, August 2023.
14f Jose J. Gorbea Colón†, Leon Palao III†, Shin-Fu Chen, Hee Jong Kim, Laura Snyder, Yi-Wei Chang*, Kuang-Lei Tsai*, Kenji Murakami*: Structural basis of a transcription pre-initiation complex on a divergent promoter. Molecular Cell 83: 574-588, February 2023.
19a Matthew Martinez, William David Chen, Marta Cova, Petra Andrea Molnár, Shrawan Kumar Mageswaran, Amandine Guérin, Audrey Ragan Odom John, Maryse Lebrun, Yi-Wei Chang*: Rhoptry secretion system structure and priming in Plasmodium falciparum revealed using in situ cryo-electron tomography. Nature Microbiology 7: 1230-1238, August 2022.
136 Liam Theveny†, Shrawan Kumar Mageswaran†, William David Chen, Matthew Martinez, Amandine Guérin, Yi-Wei Chang*: Parasitology meets cryo-electron tomography - exciting prospects await. Trends in Parasitology 38(5): 365-378, May 2022.
16d Shrawan Kumar Mageswaran, Amandine Guérin†, Liam M. Theveny†, William David Chen†, Matthew Martinez, Maryse Lebrun, Boris Striepen, Yi-Wei Chang*: In situ ultrastructures of two evolutionarily distant apicomplexan rhoptry secretion systems. Nature Communications 12: 4983, August 2021.
240 Eleonora Aquilini†, Marta Mendonça Cova†, Shrawan Kumar Mageswaran, Nicolas Dos Santos Pacheco, Daniela Sparvoli, Diana Marcela Penarete-Vargas, Rania Najm, Arnault Graindorge, Catherine Suarez, Marjorie Maynadier, Laurence Berry-Sterkers, Serge Urbach, Pilar Ruga Fahy, Amandine Guérin, Boris Striepen, Jean-François Dubremetz, Yi-Wei Chang, Aaron Turkewitz, Maryse Lebrun*: An Alveolata secretory machinery adapted to parasite host cell invasion. Nature Microbiology 6: 425–434, January 2021.
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Selected Publications
18e Ruchao Peng†, Xin Xu†, Binod Nepal, Yikang Gong‡, Fenglin Li‡, Max B. Ferretti, Mingyang Zhou, Kristen W. Lynch, George M. Burslem, Sandhya Kortagere, Ronen Marmorstein, Yi-Wei Chang*: Molecular basis of influenza ribonucleoprotein complex assembly and processive RNA synthesis. Science 388(6748): eadq7597, May 2025.10f Kathryn Kixmoeller, Ekaterina V. Tarasovetc, Elie Mer, Yi-Wei Chang*, Ben E Black*: Centromeric chromatin clearings demarcate the site of kinetochore formation. Cell 188(5): 1280-1296, January 2025.
13d Benjamin C Creekmore†, Kathryn Kixmoeller†, Ben E Black, Edward B Lee*, Yi-Wei Chang*: Ultrastructure of human brain tissue vitrified from autopsy revealed by cryo-ET with cryo-plasma FIB milling. Nature Communications 15: 2660, March 2024.
1ca Nicolas Dos Santos Pacheco†, Albert Tell i Puig†, Amandine Guérin†, Matthew Martinez, Bohumil Maco, Nicolò Tosetti, Estefanía Delgado-Betancourt, Matteo Lunghi, Boris Striepen, Yi-Wei Chang*, Dominique Soldati-Favre*: Sustained rhoptry docking and discharge requires Toxoplasma gondii intraconoidal microtubule-associated proteins. Nature Communications 15: 379, January 2024.
1a5 Matthew Martinez†, Shrawan Kumar Mageswaran†, Amandine Guérin, William David Chen, Cameron Thompson, Sarbin Chavin, Dominique Soldati-Favre, Boris Striepen, Yi-Wei Chang*: Origin and arrangement of actin filaments for gliding motility in apicomplexan parasites revealed by cryo-electron tomography. Nature Communications 14: 4800, August 2023.
14f Jose J. Gorbea Colón†, Leon Palao III†, Shin-Fu Chen, Hee Jong Kim, Laura Snyder, Yi-Wei Chang*, Kuang-Lei Tsai*, Kenji Murakami*: Structural basis of a transcription pre-initiation complex on a divergent promoter. Molecular Cell 83: 574-588, February 2023.
19a Matthew Martinez, William David Chen, Marta Cova, Petra Andrea Molnár, Shrawan Kumar Mageswaran, Amandine Guérin, Audrey Ragan Odom John, Maryse Lebrun, Yi-Wei Chang*: Rhoptry secretion system structure and priming in Plasmodium falciparum revealed using in situ cryo-electron tomography. Nature Microbiology 7: 1230-1238, August 2022.
136 Liam Theveny†, Shrawan Kumar Mageswaran†, William David Chen, Matthew Martinez, Amandine Guérin, Yi-Wei Chang*: Parasitology meets cryo-electron tomography - exciting prospects await. Trends in Parasitology 38(5): 365-378, May 2022.
16d Shrawan Kumar Mageswaran, Amandine Guérin†, Liam M. Theveny†, William David Chen†, Matthew Martinez, Maryse Lebrun, Boris Striepen, Yi-Wei Chang*: In situ ultrastructures of two evolutionarily distant apicomplexan rhoptry secretion systems. Nature Communications 12: 4983, August 2021.
240 Eleonora Aquilini†, Marta Mendonça Cova†, Shrawan Kumar Mageswaran, Nicolas Dos Santos Pacheco, Daniela Sparvoli, Diana Marcela Penarete-Vargas, Rania Najm, Arnault Graindorge, Catherine Suarez, Marjorie Maynadier, Laurence Berry-Sterkers, Serge Urbach, Pilar Ruga Fahy, Amandine Guérin, Boris Striepen, Jean-François Dubremetz, Yi-Wei Chang, Aaron Turkewitz, Maryse Lebrun*: An Alveolata secretory machinery adapted to parasite host cell invasion. Nature Microbiology 6: 425–434, January 2021.
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