Colin Conine, PhD
Assistant Professor of Pediatrics (Neonatology & Newborn Services)
Core Member, Penn Epigenetics Institute, University of Pennsylvania
Faculty, Penn Institute for Regenerative Medicine, University of Pennsylvania
Faculty, Center for Research on Reproduction and Women's Health, Children's Hospital of Philadelphia
Scientist, Children's Hospital of Philadelphia
Investigator, Center of Excellence in Environmental Toxicology, Children's Hospital of Philadelphia
Department: Pediatrics
Graduate Group Affiliations
Contact information
University of Pennsylvania School of Medicine
BRB II/III 1309, 421 Curie Blvd
Philadelphia, PA 19104
BRB II/III 1309, 421 Curie Blvd
Philadelphia, PA 19104
Lab: 215-573-1720
Email:
conine@upenn.edu
conine@upenn.edu
Publications
Education:
BS (Biochemistry)
University of Rochester, 2007.
PhD (Molecular Biology and Genetics)
University of Massachusetts Medical School, 2014.
Permanent linkBS (Biochemistry)
University of Rochester, 2007.
PhD (Molecular Biology and Genetics)
University of Massachusetts Medical School, 2014.
Description of Research Expertise
Research Interests:The functions of small RNAs in fertility, inheritance, and development.
Key words: RNA, sperm, embryogenesis, fertility, epigenetics, microRNAs, epididymis, extracellular vesicles
Research Details:
We are particularly interested in how RNAs present in sperm are capable of transmitting non-genetic information to their progeny, influencing offspring phenotype. This includes, 1) how small RNAs regulate gene expression in the male germline to support spermatogenesis and fertility, 2) how small RNAs are packaged into mature sperm, 3) how RNAs transmitted during fertilization are able to regulate early embryonic gene expression and development, and 4) how this regulation can alter developmental programs to produce a non-genetically inherited phenotype. The lab utilizes a combination of state-of-the-art genomic, molecular biology, and assisted reproduction techniques across multiple model systems including mice, embryonic stem cells, and C. elegans to understand these questions.
Rotation Projects:
The functions of sperm-transmitted small RNAs on embryonic gene expression and development in mice
C. elegans as a model to understand paternal epigenetic inheritance
Soma-to-germline communication of RNAs from the epididymis to sperm in mammals
Conservation of sperm RNAs across species
Novel imprinted genes in early embryonic development in mice
The effect of assisted reproduction technologies (ART) on embryonic development and offspring phenotype
Selected Publications
Golden TN, Mani S, Linn RL, Leite R, Trigg NA, Wilson A, Anton L, Mainigi M, Conine CC, Kaufman BA, Strauss JF 3rd, Parry S, Simmons RA.: Extracellular Vesicles Alter Trophoblast Function in Pregnancies Complicated by COVID-19. J Extracell Vesicles. 14(4): e70051, April 2025Trigg NA, Zhou SK, Harris JC, Lamonica MN, Nelson MA, Silverman MA, Kambayashi T, Conine CC.: A lack of commensal microbiota influences the male reproductive tract intergenerationally in mice. Reproduction. 169(4): e240204, March 2025 Notes: doi: 10.1530/REP-24-0204.
Scacchetti A, Shields EJ, Trigg NA, Wilusz JE, Conine CC, Bonasio R.: A ligation-independent sequencing method reveals tRNA-derived RNAs with blocked 3' termini. Molecular Cell 84(19): 3843-3859, October 2024.
Trigg, Natalie A., Conine, Colin C.: Epididymal acquired sperm microRNAs modify post-fertilization embryonic gene expression. Cell Reports 43(9): 114698, September 2024.
Harris JC, Trigg NA, Goshu B, Yokoyama Y, Dohnalová L, White EK, Harman A, Murga-Garrido SM, Ting-Chun Pan J, Bhanap P, Thaiss CA, Grice EA, Conine CC, Kambayashi T.: The microbiota and T cells non-genetically modulate inherited phenotypes transgenerationally. Cell Reports 43(4): 114029, April 2024.
Senaldi L, Hassan N, Cullen S, Balaji U, Trigg N, Gu J, Finkelstein H, Phillips K, Conine C, Smith-Raska M.: Khdc3 Regulates Metabolism Across Generations in a DNA-Independent Manner. bioRxiv Page: doi: 10.1101, March 2024 Notes: epub ahead of print.
Golden, Thea N., Mani, Sneha, Linn, Rebecca L., Leite, Rita, Trigg, Natalie A., Wilson, Annette, Anton, Lauren, Mainigi, Monica, Conine, Colin C., Kaufman, Brett A., Strauss, Jerome F., Parry, Samuel, Simmons, Rebecca A.: Extracellular vesicles alter trophoblast function in pregnancies complicated by COVID-19. bioRxiv Page: doi.org/10.1101/2024.02.17.580824, February 2024 Notes: epub.
Harris JC, Trigg NA, Goshu B, Yokoyama Y, Dohnalová L, White EK, Harman A, Thaiss CA, Grice EA, Conine CC, Kambayashi T.: The microbiota and immune system non-genetically affect offspring phenotypes transgenerationally. bioRxiv Page: doi.org/10.1101/2023.04.06.535940, April 2023 Notes: epub.
Crocker OJ, Trigg NA, Conine CC.: Cloning and Sequencing Eukaryotic Small RNAs. Curr Protoc 2(8): e495, August 2022.
Lee GS, Conine CC.: The Transmission of Intergenerational Epigenetic Information by Sperm microRNAs. Epigenomes 6(2): 12, April 2022.