1e
In Memoriam
16
19
1
49
2
2
18
1b
1d
18
37
2b
7
8a
1d
2 29
1d
25
Randall N. Pittman, Ph.D.
78
2b
7
8a
Department: Systems Pharmacology and Translational Therapeutics
4
1
b
1d
46
Contact information
48
4
3
3
1d
48
Department of Pharmacology
18 154A John Morgan
3a 3620 Hamilton Walk
Philadelphia, PA 19104-6084
26
18 154A John Morgan
3a 3620 Hamilton Walk
Philadelphia, PA 19104-6084
31
Office: (215) 898-9736
35 Fax: (215) 573-2236
24
95
35 Fax: (215) 573-2236
24
Email:
pittman@mail.med.upenn.edu
12
pittman@mail.med.upenn.edu
18
Publications
23 a
3
2
29
23 a
Links
1e9 Search PubMed for articles
41 Faculty Profile, Graduate Group in Neuroscience, University of Pennsylvania School of Medicine
42 Faculty Profile, Graduate Group in Pharmacological Sciences, University of Pennsylvania School of Medicine
98 Pittman Laboratory Homepage, University of Pennsylvania School of Medicine
c
4
b
1f
1e9 Search PubMed for articles
41 Faculty Profile, Graduate Group in Neuroscience, University of Pennsylvania School of Medicine
42 Faculty Profile, Graduate Group in Pharmacological Sciences, University of Pennsylvania School of Medicine
98 Pittman Laboratory Homepage, University of Pennsylvania School of Medicine
c
13
Education:
21 9 B.S. 16 (Chemistry) c
46 University of North Carolina, Chapel Hill, NC, 1972.
21 a Ph.D. 19 (Pharmacology) c
3b University of Colorado, Denver, CO, 1981.
c
3
3
3
3
8a
Permanent link21 9 B.S. 16 (Chemistry) c
46 University of North Carolina, Chapel Hill, NC, 1972.
21 a Ph.D. 19 (Pharmacology) c
3b University of Colorado, Denver, CO, 1981.
c
2 29
21
1e
1d
24
5e
79 Cellular and molecular events in neural apoptosis and polyglutamine repeat expansion neurodegenerative diseases.
8
1b RESEARCH TECHNIQUES
7e Cellular and molecular events regulating apoptosis and cellular pathology in polyglutamine neurodegenerative diseases
8
18 RESEARCH SUMMARY
483 Cellular and molecular approaches are used to study signaling pathways controlling cell survival, cell death (apoptosis), and early nuclear events in polyglutamine neurodegenerative diseases. The two major projects in the lab are: 1) characterizing signaling pathways in the execution phase of apoptosis, and 2) investigating the relationship between CAG trinucleotide repeat proteins, the nuclear environment, the ubiquitin/proteasome machinery, and neuronal dysfunction/death. Apoptosis experiments are focused on understanding cellular events controlling signal transduction in the execution phase with particular emphasis on cytoskeletal changes associated with entry into the execution phase and dynamic membrane blebbing. Other studies on apoptosis are focused on kinase signaling at the interface of cell survival and cell death. Studies on the polyglutamine disease protein, ataxin-3, are currently focused on the relationship between the nuclear environment, transcriptional regulation, ubiquitination and proteasome system and neuronal dysfunction/death using cellular and animal models and reconstituted cellular and biochemical systems.
26 29
27
Description of Research Expertise
23 RESEARCH INTERESTS79 Cellular and molecular events in neural apoptosis and polyglutamine repeat expansion neurodegenerative diseases.
8
1b RESEARCH TECHNIQUES
7e Cellular and molecular events regulating apoptosis and cellular pathology in polyglutamine neurodegenerative diseases
8
18 RESEARCH SUMMARY
483 Cellular and molecular approaches are used to study signaling pathways controlling cell survival, cell death (apoptosis), and early nuclear events in polyglutamine neurodegenerative diseases. The two major projects in the lab are: 1) characterizing signaling pathways in the execution phase of apoptosis, and 2) investigating the relationship between CAG trinucleotide repeat proteins, the nuclear environment, the ubiquitin/proteasome machinery, and neuronal dysfunction/death. Apoptosis experiments are focused on understanding cellular events controlling signal transduction in the execution phase with particular emphasis on cytoskeletal changes associated with entry into the execution phase and dynamic membrane blebbing. Other studies on apoptosis are focused on kinase signaling at the interface of cell survival and cell death. Studies on the polyglutamine disease protein, ataxin-3, are currently focused on the relationship between the nuclear environment, transcriptional regulation, ubiquitination and proteasome system and neuronal dysfunction/death using cellular and animal models and reconstituted cellular and biochemical systems.
26 29
23
cf Zhong X and Pittman RN: Ataxin-3 binds VCP/p97 and regulates retrotranslocation of ERAD substrates Hum Mol Genet 15: 2409-2420, 2006.
e1 Bilen J, Liu N, Burnett BG, Pittman RN, Bonini NM: MicroRNA pathways modulate polyglutamine-induced neurodegeneration. Molecular Cell 24: 157-163, 2006.
e4 Burnett BG and Pittman RN: The polyglutamine neurodegenerative protein ataxin 3 regulates aggresome formation. Proc Natl Acad Sci USA 102: 4330-4335, 2005.
103 Burnett B, Li F, Pittman RN: The polyglutamine neurodegenerative protein ataxin-3 binds polyubiquitylated proteins and has ubiquitin protease activity. Hum Mol Genet 12: 3195-3205, 2003.
120 Li F, Macfarlan T, Pittman* RN, Chakravarti* D: Ataxin-3 is a histone binding protein with two independent transcriptional corepressor activities. J Biol Chem 277: 45004-45012, 2002 Notes: *Co-communicating authors.
d7 Zhou H, Li XM, Meinkoth J, Pittman RN: Akt regulates cell survival and apoptosis at a postmitochondrial level. J Cell Biol 151: 483-494, 2000.
da Tang Y, Zhou H, Chen A, Pittman RN, Field J: The Akt proto-oncogene links Ras to Pak and cell survival signals. J Biol Chem 275: 9106-9109, 2000.
ff Perez MK, Paulson HL, Pittman RN: Ataxin-3 with an altered conformation that exposes the polyglutamine domain is associated with the nuclear matrix. Hum Mol Genet 8: 2377-2385, 1999.
d2 Mills JC, Stone NL, Pittman RN: Extranuclear apoptosis. The role of the cytoplasm in the execution phase. J Cell Biol 146: 703-708, 1999.
2c
7
1d
1f
Selected Publications
d2 Orlando KA, Stone NL, Pittman RN: Rho kinase regulates fragmentation and phagocytosis of apoptotic cells. Exper Cell Res 312: 5-15, 2006.cf Zhong X and Pittman RN: Ataxin-3 binds VCP/p97 and regulates retrotranslocation of ERAD substrates Hum Mol Genet 15: 2409-2420, 2006.
e1 Bilen J, Liu N, Burnett BG, Pittman RN, Bonini NM: MicroRNA pathways modulate polyglutamine-induced neurodegeneration. Molecular Cell 24: 157-163, 2006.
e4 Burnett BG and Pittman RN: The polyglutamine neurodegenerative protein ataxin 3 regulates aggresome formation. Proc Natl Acad Sci USA 102: 4330-4335, 2005.
103 Burnett B, Li F, Pittman RN: The polyglutamine neurodegenerative protein ataxin-3 binds polyubiquitylated proteins and has ubiquitin protease activity. Hum Mol Genet 12: 3195-3205, 2003.
120 Li F, Macfarlan T, Pittman* RN, Chakravarti* D: Ataxin-3 is a histone binding protein with two independent transcriptional corepressor activities. J Biol Chem 277: 45004-45012, 2002 Notes: *Co-communicating authors.
d7 Zhou H, Li XM, Meinkoth J, Pittman RN: Akt regulates cell survival and apoptosis at a postmitochondrial level. J Cell Biol 151: 483-494, 2000.
da Tang Y, Zhou H, Chen A, Pittman RN, Field J: The Akt proto-oncogene links Ras to Pak and cell survival signals. J Biol Chem 275: 9106-9109, 2000.
ff Perez MK, Paulson HL, Pittman RN: Ataxin-3 with an altered conformation that exposes the polyglutamine domain is associated with the nuclear matrix. Hum Mol Genet 8: 2377-2385, 1999.
d2 Mills JC, Stone NL, Pittman RN: Extranuclear apoptosis. The role of the cytoplasm in the execution phase. J Cell Biol 146: 703-708, 1999.
2c