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Gerd A. Blobel, MD, PhD

Gerd A. Blobel, MD, PhD

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Professor of Pediatrics
Department: Pediatrics
Graduate Group Affiliations

Contact information
316H Abramson Research Center
3615 Civic Center Blvd.
Philadelphia, PA 19104
Office: (215) 590-3988
Fax: (215) 590-4834
Lab: (215) 590-4461
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Description of Research Expertise

Research Interests
hematopoiesis, higher order chromatin structure, gene expression, epigenetic memory, transcription factors

Description of Research
Our lab focuses on the genetic and epigenetic control of hematopoiesis and its disorders. Specifically, we study how tissue-specific transcription factors govern the specification and maintenance of hematopoietic cell lineages. We also examine how transcription programs are epigenetically transmitted through mitosis to maintain lineage identity. Our work is leading us deeply into the analysis of chromatin structure, its modifications and organization. For example, we investigate how regulatory elements are organized spatially using chromosome conformation capture technology. We are also interested in the control of transcription factors by posttranslational modifications. For our studies we combine molecular, genomic, biochemical and imaging approaches with studies in normal and gene targeted mice.

Lab Rotation Projects:
1) Regulation of higher order chromatin organization during gene activation and repression
2) Mechanisms of epigenetic bookmarking during mitosis
3) Lineage choice decisions by GATA1, FOG1 and Ets transcription factors during hematopoiesis
4) Molecular mechanisms of GATA1 acetylation
5) Linking GATA1 mutations to molecular disease pathways

Lab personnel:
Amy Campbell, graduate student
Chris Hsiung, combined degree student
Sarah Hsu combined degree student
Aaron Stonestrom, combined degree student
Rena Zheng, combined degree student
Wulan Deng, postdoctoral fellow
Chris Edwards, postdoctoral fellow
Peng Huang, postdoctoral fellow
Kiwon Lee, postdoctoral fellow
Jeremy Rupon, postdoctoral fellow
Suzi Shapira rotation student
Hongxin Wang, technician/lab manager

Selected Publications

Jing H, Vakoc CR, Ying L, Mandat S, Wang H, Zheng X, Blobel GA: Exchange of GATA factors mediates transitions in looped chromatin organization at a developmentally regulated gene locus. Molecular Cell 29: 232, 2008.

Blobel GA, Kadauke S, Wang E, Lau AW, Zuber J, Chou MM, Vakoc CR: A reconfigured pattern of MLL occupancy within mitotic chromatin promotes rapid transcriptional reactivation following mitotic exit. Molecular Cell 36: 970, 2009.

Gregory GD, Miccio A, Bersenev A, Wang Y, Hong W, Zhang Z, Poncz M, Tong W, Blobel GA: FOG1 requires NuRD to promote hematopoiesis and maintain lineage fidelity within the megakaryocytic-erythroid compartment. Blood 115: 2156, 2010.

Miccio A, Blobel GA: The role of the GATA-1/FOG-1/NuRD pathway in the expression of human {beta}-like globin genes. Mol. Cell. Biol. 30: 3460, 2010.

Deng W, Blobel GA: Do chromatin loops provide epigenetic gene expression states? Curr. Opin. Genet. Dev. 20: 548, 2010.

Miccio A, Wang Y, Hong W, Gregory GD, Wang H, Yu X, Choi JK, Shelat S, Tong W, Poncz M, Blobel GA: NuRD mediates activating and repressive functions of GATA-1 and FOG-1 during blood development. EMBO J. 29: 442, 2010.

Gamsjaeger R, Webb SR, Lamonica JM, Billin A, Blobel GA, Mackay JP: Structural Basis and Specificity of Acetylated Transcription Factor GATA1 Recognition by BET Family Bromodomain Protein Brd3. Mol. Cell. Biol. 31: 2632, 2011.

Lamonica JM, Deng W, Kadauke S, Campbell AE, Gamsjaeger R, Wang H, Cheng Y, Billin AN, Hardison RC, Mackay JP, Blobel GA: Bromodomain protein Brd3 associates with acetylated GATA1 to promote its chromatin occupancy at erythroid target genes. PNAS 108: 159, 2011.

Deng W, Lee J, Wang H, Miller J, Reik A, Gregory PD, Dean A, Blobel GA: Controlling long-range genomic interactions at a native locus by targeted tethering of a looping factor. Cell 149: 1233, 2012.

Kadauke S, Udugama MI, Pawlicki JM, Achtman JC,Jain DP, Cheng Y, Hardison RC, and Blobel GA: Tissue-specific Mitotic Bookmarking by Hematopoietic Transcription Factor GATA1. Cell 150: 725, 2012.

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Last updated: 05/19/2013
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