Nicolai M Doliba, D.Sc., Ph.D.
Research Associate Professor of Biochemistry and Biophysics
Technical Director of the Pancreatic Islet Cell Biology Core, NIH funded Diabetes Research Center, Institute for Diabetes, Obesity and Metabolism, University of Pennsylvania, Philadelphia
Department: Biochemistry and Biophysics
Contact information
12-115 Smilow Translational Research Center
3400 Civic Center Boulevard
Philadelphia, PA 19104-5160
3400 Civic Center Boulevard
Philadelphia, PA 19104-5160
Office: 215-898-4366
Fax: 215-898-5408
Lab: 215-898-4366
Fax: 215-898-5408
Lab: 215-898-4366
Publications
Education:
B.S. (Biology)
Ivan Franko National University of Lviv, Lviv, Ukraine, 1982.
Ph.D. (Physiology)
Lviv Medical University, Lviv, Ukraine, 1986.
D.Sc. (Physiology)
Ivan Franko National University of Lviv, Lviv, Ukraine, 1993.
Permanent linkB.S. (Biology)
Ivan Franko National University of Lviv, Lviv, Ukraine, 1982.
Ph.D. (Physiology)
Lviv Medical University, Lviv, Ukraine, 1986.
D.Sc. (Physiology)
Ivan Franko National University of Lviv, Lviv, Ukraine, 1993.
Description of Research Expertise
My research has focused on the role of bioenergetics, ion transport and metabolic coupling factors in nutrient- and drug-stimulated insulin release at normal conditions and during diabetes mellitus.Using combinations of different methods and approaches, including 31P, 13C, and 1H Nuclear Magnetic Resonance Spectroscopy techniques, polarographic and phosphorescence methods of measuring O2 consumption, Ca2+ imaging and others, I attempt to directly address the essential mechanisms of stimulus-secretion coupling in pancreatic islets.
Selected Publications
Doliba NM, Rozo AV, Roman J, Qin W, Traum D, Gao L, Liu J, Manduchi E, Liu C, Golson ML, Vahedi G, Naji A, Matschinsky FM, Atkinson MA, Powers AC, Brissova M, Kaestner KH, Stoffers DA; HPAP Consortium: α Cell dysfunction in islets from nondiabetic, glutamic acid decarboxylase autoantibody-positive individuals. J Clin Invest 132(11): e156243, Jun 2022.Zhu L, Dattaroy D, Pham J, Wang L, Barella LF, Cui Y, Wilkins KJ, Roth BL, Hochgeschwender U, Matschinsky FM, Kaestner KH, Doliba NM, Wess J: Intra-islet glucagon signaling is critical for maintaining glucose homeostasis. JCI Insight 5: e127994, Apr 2019.
Zhu L, Almaça J, Dadi PK, Hong H, Sakamoto W, Rossi M, Lee RJ, Vierra NC, Lu H, Cui Y, McMillin SM, Perry NA, Gurevich VV, Lee A, Kuo B, Leapman RD, Matschinsky FM, Doliba NM, Urs NM, Caron MG, Jacobson DA, Caicedo A, Wess J: β-arrestin-2 is an essential regulator of pancreatic β-cell function under physiological and pathophysiological conditions. Nature communications 8: 14295, Feb 2017.
Doliba NM, Liu Q, Li C, Chen P, Liu C, Naji A, Matschinsky FM: Inhibition of cholinergic potentiation of insulin secretion from pancreatic islets by chronic elevation of glucose and fatty acids: Protection by casein kinase 2 inhibitor. Mol Metab 6(10), Oct 2017.
Matyshevsky O, Doliba N, Babsky A, Vatamaniuk M, Kachur A, Badjuk O: Scientific translation from english to ukrainian: Lehninger: principles of biochemistry. Fourth Edition Lehninger: Principles of Biochemistry. BaK, 4: 1-1256, 2016.
Rossi M, RuizDeAzua I, Barella LF, Sakamoto W, Zhu L, Cui Y, Lu H, Rebholz H, Matschinsky FM, Doliba NM, Butcher AJ, Tobin AB, Wess J: CK2 acts as a potent negative regulator of receptor-mediated insulin release in vitro and in vivo. Proceedings of the National Academy of Sciences of the United States of America 112(49): E6818-24, Dec 2015.
Doliba NM, Liu Q, Li C, Chen J, Chen P, Liu C, Frederick DW, Baur JA, Bennett MJ, Naji A, Matschinsky FM: Accumulation of 3-hydroxytetradecenoic acid: Cause or corollary of glucolipotoxic impairment of pancreatic β-cell bioenergetics? Molecular metabolism 4(12): 926-39, Dec 2015.
Doliba NM, Qin W, Najafi H, Liu C, Buettger CW, Sotiris J, Collins HW, Li C, Stanley CA, Wilson DF, Grimsby J, Sarabu R, Naji A, Matschinsky FM: Glucokinase activation repairs defective bioenergetics of islets of Langerhans isolated from type 2 diabetics. American journal of physiology 302(1): E87-E102, Jan 2012.
Doliba NM, Qin W, Vinogradov SA, Wilson DF, Matschinsky FM: Palmitic acid acutely inhibits acetylcholine- but not GLP-1-stimulated insulin secretion in mouse pancreatic islets. American Journal of Physiology - Endocrinology & Metabolism 299(3): E475-85, Sep 2010.
Doliba NM, Wehrli SL, Vatamaniuk M, Qin W, Buettger CW, Collins HW, Matschinsky FM: Metabolic and ionic coupling factors in amino acid-stimulated insulin release in pancreatic beta-HC9 cells. American Journal of Physiology - Endocrinology & Metabolism 292(6): E1507-19, Jun 2007.