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Kushol Gupta, Ph.D.
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Research Assistant Professor of Biochemistry and Biophysics
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Member, Center for AIDs Research (CFAR), University of Pennsylvania
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Director, Johnson Foundation Structural Biology and Biophysics Core Facility, Department of Biochemistry and Biophysics, Perelman School of Medicine
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Member, Institute for Structural Biology, Perelman School of Medicine, University of Pennsylvania
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Member, Institute for RNA Biology, Perelman School of Medicine, University of Pennsylvania
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Department: Biochemistry and Biophysics
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Graduate Group Affiliations
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Contact information
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901C Stellar-Chance Building
17 422 Curie Blvd.
4c Department of Biochemistry and Biophysics
Philadelphia, PA 19104
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17 422 Curie Blvd.
4c Department of Biochemistry and Biophysics
Philadelphia, PA 19104
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Office: 215-898-0816
3e Lab: 215-573-7260
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3e Lab: 215-573-7260
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Publications
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Links
b6 Search PubMed for articles
91 Johnson Foundation Biophysics and Structural Biology Core Facility
5f Research Website
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b6 Search PubMed for articles
91 Johnson Foundation Biophysics and Structural Biology Core Facility
5f Research Website
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Education:
21 9 B.A. 23 (Biochemistry and Music) c
33 University of Pennsylvania, 1997.
21 a Ph.D. 19 (Pharmacology) c
50 Perelman School of Medicine, University of Pennsylvania, 2003.
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Permanent link21 9 B.A. 23 (Biochemistry and Music) c
33 University of Pennsylvania, 1997.
21 a Ph.D. 19 (Pharmacology) c
50 Perelman School of Medicine, University of Pennsylvania, 2003.
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3b The Gupta Lab focuses on three main research areas:
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95 Enzymes that underlie inborn errors of metabolism, including phenylalanine hydroxylase, cystathionine beta-synthase, and pantothenate kinase.
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be Retroviral integrases, their interactions with host factors, and a novel class of antiviral drugs known as allosteric integrase inhibitors (ALLINIs), which are effective against HIV.
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d0 Biophysical studies of mRNA lipid nanoparticles (mRNA LNPs), including emerging modalities of small angle X-ray and neutron scattering, multiangle light scattering, and analytical ultracentrifugation.
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b9 Additional research spans RNA splicing, viral proteins, chromatin, and site-specific recombination, reflecting the collaborative and interdisciplinary nature of research at Penn.
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Description of Research Expertise
24a Kushol Gupta is a Research Assistant Professor in the Department of Biochemistry & Biophysics at the Perelman School of Medicine of The University of Pennsylvania. He is a member of the BMB graduate group, and directs the Johnson Foundation Structural Biology and Biophysics Core, a departmental resource that serves Penn and the greater region. He is a structural biologist with expertise in both X-ray crystallography and solution-based biophysical techniques, including small-angle X-ray and neutron scattering, light scattering, and analytical ultracentrifugation.8
3b The Gupta Lab focuses on three main research areas:
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95 Enzymes that underlie inborn errors of metabolism, including phenylalanine hydroxylase, cystathionine beta-synthase, and pantothenate kinase.
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be Retroviral integrases, their interactions with host factors, and a novel class of antiviral drugs known as allosteric integrase inhibitors (ALLINIs), which are effective against HIV.
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d0 Biophysical studies of mRNA lipid nanoparticles (mRNA LNPs), including emerging modalities of small angle X-ray and neutron scattering, multiangle light scattering, and analytical ultracentrifugation.
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b9 Additional research spans RNA splicing, viral proteins, chromatin, and site-specific recombination, reflecting the collaborative and interdisciplinary nature of research at Penn.
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155 Eilers G, Gupta K, Allen A, Murali H, Sharp R, Hwang Y, Bushman FD, Van Duyne GD. : Structure of a Minimal HIV-1 IN-Allosteric Inhibitor Complex at 2.93 Å Resolutions: Routes to Inhibitor Optimization. PLoS Pathogens 19(3): e1011097, Mar 2023 Notes: co-First Author.
11e Arturo EC, Merkel GW, Hansen MR, Lisowski S, Almeida D, Gupta K, Jaffe EK.: Manipulation of a Cation-Π Sandwich Reveals Conformational Flexibility in Phenylalanine Hydroxylase. Biochimie 183(63): 63-77, Apr 2021.
177 Gupta K, Allen A, Giraldo C, Eilers G, Sharp R, Hwang Y, Murali H, Cruz K, Janmey P, Bushman F, Van Duyne GD.: Allosteric HIV Integrase Inhibitors Promote Formation of Inactive Branched Polymers via Homomeric Carboxy-Terminal Domain Interactions. Structure 29: 213-225, Mar 2021 Notes: Editor's Choice.
178 Gupta K, Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG.: Assembly of Higher-Order SMN Oligomers is Essential for Metazoan Viability and Requires an Exposed Structural Motif Present in the YG Zipper Dimer. Nucleic Acids Research 49: 7644-7664, Jul 2021.
163 Arturo, E. C., Gupta, K., Hansen, M. R., Borne, E., Jaffe, E. K.: Biophysical Characterization of Full-Length Human Phenylalanine Hydroxylase Provides a Deeper Understanding of its Quaternary Structure Equilibrium. Journal of Biological Chemistry 294(26): 10131-10145, Jun 2019.
2b9 Ashkar R, Bilheux HZ, Bordallo HN, Briber RM, Callaway DJE, Cheng X, Chu XQ, Curtis JE, Dadmun M, Fenimore PW, Fushman D, Gabel F, Gupta K, Heberle FA, Heinrich F, Hong L, Katsaras J, Kelman Z, Kharlampieva E, Kneller GR, Kovalevsky A, Krueger S, Langan P, Liberman RL, Liu Y, Losche M, Lyman E, Mao Y, Marino JP, Mattos C, Meilleur F, Moody PCE, Nickels JD, O’Neill H, Perez-Salas U, Peters J, Petridis L, Sokolov AP, Wager NJ, Weinrich M, Wymore T, Zhang Y, Smith JC.: Progress and Prospects for Neutron Scattering in Biological Sciences. Acta Crystallographica Section D Structural Biology 74(12): 1129-1168, Dec 2018.
10a Gupta Kushol, Sharp Robert, Yuan Jimmy B, Li Huiguang, Van Duyne Gregory D: Coiled-Coil Interactions Mediate Serine Integrase Directionality. Nucleic Acids Research 45(12): 7339-7353, Jul 2017.
1a9 Arturo Emilia C, Gupta Kushol, Héroux Annie, Stith Linda, Cross Penelope J, Parker Emily J, Loll Patrick J, Jaffe Eileen K: First Structure of Full-Length Mammalian Phenylalanine Hydroxylase Reveals the Architecture of an Autoinhibited Tetramer. Proceedings of the National Academy of Sciences of the United States of America 113(9): 2394-9, Mar 2016.
196 Gupta Kushol, Turkki Vesa, Sherrill-Mix Scott, Hwang Young, Eilers Grant, Taylor Louis, McDanal Charlene, Wang Ping, Temelkoff David, Nolte Robert T, Velthuisen Emile, Jeffrey Jerry, Van Duyne Gregory D, Bushman Frederic D: Structural Basis for Inhibitor-Induced Aggregation of HIV Integrase. PLoS biology 14(12): e1002584, Dec 2016.
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Selected Publications
127 Lee HO, Wang L, Gupta K, Dunbrack RL, Majtan T, Kruger WD. : Impact of Primary Sequence Changes on the Self-Association Properties of Mammalian Cystathionine Beta-Synthase Enzymes. Protein Science 33(12): e5223, Dec 2024.155 Eilers G, Gupta K, Allen A, Murali H, Sharp R, Hwang Y, Bushman FD, Van Duyne GD. : Structure of a Minimal HIV-1 IN-Allosteric Inhibitor Complex at 2.93 Å Resolutions: Routes to Inhibitor Optimization. PLoS Pathogens 19(3): e1011097, Mar 2023 Notes: co-First Author.
11e Arturo EC, Merkel GW, Hansen MR, Lisowski S, Almeida D, Gupta K, Jaffe EK.: Manipulation of a Cation-Π Sandwich Reveals Conformational Flexibility in Phenylalanine Hydroxylase. Biochimie 183(63): 63-77, Apr 2021.
177 Gupta K, Allen A, Giraldo C, Eilers G, Sharp R, Hwang Y, Murali H, Cruz K, Janmey P, Bushman F, Van Duyne GD.: Allosteric HIV Integrase Inhibitors Promote Formation of Inactive Branched Polymers via Homomeric Carboxy-Terminal Domain Interactions. Structure 29: 213-225, Mar 2021 Notes: Editor's Choice.
178 Gupta K, Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG.: Assembly of Higher-Order SMN Oligomers is Essential for Metazoan Viability and Requires an Exposed Structural Motif Present in the YG Zipper Dimer. Nucleic Acids Research 49: 7644-7664, Jul 2021.
163 Arturo, E. C., Gupta, K., Hansen, M. R., Borne, E., Jaffe, E. K.: Biophysical Characterization of Full-Length Human Phenylalanine Hydroxylase Provides a Deeper Understanding of its Quaternary Structure Equilibrium. Journal of Biological Chemistry 294(26): 10131-10145, Jun 2019.
2b9 Ashkar R, Bilheux HZ, Bordallo HN, Briber RM, Callaway DJE, Cheng X, Chu XQ, Curtis JE, Dadmun M, Fenimore PW, Fushman D, Gabel F, Gupta K, Heberle FA, Heinrich F, Hong L, Katsaras J, Kelman Z, Kharlampieva E, Kneller GR, Kovalevsky A, Krueger S, Langan P, Liberman RL, Liu Y, Losche M, Lyman E, Mao Y, Marino JP, Mattos C, Meilleur F, Moody PCE, Nickels JD, O’Neill H, Perez-Salas U, Peters J, Petridis L, Sokolov AP, Wager NJ, Weinrich M, Wymore T, Zhang Y, Smith JC.: Progress and Prospects for Neutron Scattering in Biological Sciences. Acta Crystallographica Section D Structural Biology 74(12): 1129-1168, Dec 2018.
10a Gupta Kushol, Sharp Robert, Yuan Jimmy B, Li Huiguang, Van Duyne Gregory D: Coiled-Coil Interactions Mediate Serine Integrase Directionality. Nucleic Acids Research 45(12): 7339-7353, Jul 2017.
1a9 Arturo Emilia C, Gupta Kushol, Héroux Annie, Stith Linda, Cross Penelope J, Parker Emily J, Loll Patrick J, Jaffe Eileen K: First Structure of Full-Length Mammalian Phenylalanine Hydroxylase Reveals the Architecture of an Autoinhibited Tetramer. Proceedings of the National Academy of Sciences of the United States of America 113(9): 2394-9, Mar 2016.
196 Gupta Kushol, Turkki Vesa, Sherrill-Mix Scott, Hwang Young, Eilers Grant, Taylor Louis, McDanal Charlene, Wang Ping, Temelkoff David, Nolte Robert T, Velthuisen Emile, Jeffrey Jerry, Van Duyne Gregory D, Bushman Frederic D: Structural Basis for Inhibitor-Induced Aggregation of HIV Integrase. PLoS biology 14(12): e1002584, Dec 2016.
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