Michael C. Milone, M.D., Ph.D.

faculty photo
Associate Professor of Pathology and Laboratory Medicine at the Hospital of the University of Pennsylvania
Department: Pathology and Laboratory Medicine
Graduate Group Affiliations

Contact information
3400 Spruce Street 7103 Founders Pavilion
Philadelphia, PA 19104
Office: 215-662-6575
Fax: 215-662-7529
B.S (Chemical Biology)
Stevens Institute of Technology, 1993.
Ph.D (Experimental Pathology/ Immunology)
UMDNJ - Graduate School of Biomedical Sciences, 1999.
M.D. (Medical Doctor)
UMDNJ- New Jersey Medical School, 1999.
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Description of Research Expertise

Research in my laboratory is focused on T cell biology and applications of genetically-engineered T cells to the treatment of disease. Active research projects in my laboratory include:

1) Developing chimeric antigen receptors (CARs) for adoptive immunotherapy of cancer with enhanced function and improved safety.

2) Developing and applying synthetic immunoreceptors to the treatment of immune-mediated disease.

3) Exploring the role of co-stimulatory signals in directing T cell metabolism and the way this metabolism affects T cell function within tumors.

Description of Clinical Expertise

Clinical Laboratory Toxicology, Therapeutic Drug Monitoring, Clinical Chemistry, Blood Banking/Transfusion Medicine, Therapeutic Apheresis

Selected Publications

Richman, S. A., Wang, L. C., Khire, U. R., Albelda, S. M., Milone, M. C.: Ligand-Induced Degradation of a CAR Permits Reversible Remote Control of CAR T Cell Activity In Vitro and In Vivo. Mol Ther June 2020.

Philipson Benjamin I, O'Connor Roddy S, May Michael J, June Carl H, Albelda Steven M, Milone Michael C: 4-1BB costimulation promotes CAR T cell survival through noncanonical NF-κB signaling. Science signaling 13(625), Mar 2020.

Sellmyer Mark A, Richman Sarah A, Lohith Katheryn, Hou Catherine, Weng Chi-Chang, Mach Robert H, O'Connor Roddy S, Milone Michael C, Farwell Michael D: Imaging CAR T Cell Trafficking with eDHFR as a PET Reporter Gene. Molecular therapy : the journal of the American Society of Gene Therapy 28(1): 42-51, Jan 2020.

O'Connor Roddy S, Milone Michael C: Testing the Specificity of Compounds Designed to Inhibit CPT1A in T Cells. Methods in molecular biology (Clifton, N.J.) 2097: 83-90, 2020.

Ghassemi Saba, Milone Michael C: Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy. Journal of visualized experiments : JoVE(154), Dec 2019.

Sheng Feng, Milone Michael C: Commentary. Clinical chemistry 65(11): 1361, Nov 2019.

Garfall Alfred L, Dancy Ehren K, Cohen Adam D, Hwang Wei-Ting, Fraietta Joseph A, Davis Megan M, Levine Bruce L, Siegel Don L, Stadtmauer Edward A, Vogl Dan T, Waxman Adam, Rapoport Aaron P, Milone Michael C, June Carl H, Melenhorst J Joseph: T-cell phenotypes associated with effective CAR T-cell therapy in postinduction vs relapsed multiple myeloma. Blood advances 3(19): 2812-2815, Oct 2019.

Feins Steven, Kong Weimin, Williams Erik F, Milone Michael C, Fraietta Joseph A: An introduction to chimeric antigen receptor (CAR) T-cell immunotherapy for human cancer. American journal of hematology 94(S1): S3-S9, May 2019.

Thibodeaux Suzanne R, Milone Michael C: Immunotherapy Using Chimeric Antigen Receptor-Engineered T Cells: A Novel Cellular Therapy with Important Implications for the Clinical Laboratory. Clinical chemistry 65(4): 519-529, April 2019.

Cohen Adam D, Garfall Alfred L, Stadtmauer Edward A, Melenhorst J Joseph, Lacey Simon F, Lancaster Eric, Vogl Dan T, Weiss Brendan M, Dengel Karen, Nelson Annemarie, Plesa Gabriela, Chen Fang, Davis Megan M, Hwang Wei-Ting, Young Regina M, Brogdon Jennifer L, Isaacs Randi, Pruteanu-Malinici Iulian, Siegel Don L, Levine Bruce L, June Carl H, Milone Michael C: B cell maturation antigen-specific CAR T cells are clinically active in multiple myeloma. The Journal of clinical investigation 130: 2210-2221, Mar 2019.

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Last updated: 11/11/2020
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