Michael C. Milone, M.D., Ph.D.

faculty photo
Associate Professor of Pathology and Laboratory Medicine at the Hospital of the University of Pennsylvania
Department: Pathology and Laboratory Medicine
Graduate Group Affiliations

Contact information
3400 Spruce Street 7103 Founders Pavilion
Philadelphia, PA 19104
Office: 215-662-6575
Fax: 215-662-7529
B.S (Chemical Biology)
Stevens Institute of Technology, 1993.
M.D. (Medical Doctor)
UMDNJ- New Jersey Medical School, 1999.
Ph.D (Experimental Pathology/ Immunology)
UMDNJ - Graduate School of Biomedical Sciences, 1999.
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Description of Research Expertise

Research in my laboratory is focused on T cell biology and applications of genetically-engineered T cells to the treatment of disease. Active research projects in my laboratory include:

1) Developing chimeric antigen receptors (CARs) for adoptive immunotherapy of cancer with enhanced function and improved safety.

2) Developing and applying synthetic immunoreceptors to the treatment of immune-mediated disease.

3) Exploring the role of co-stimulatory signals in directing T cell metabolism and the way this metabolism affects T cell function within tumors.

Description of Clinical Expertise

Clinical Laboratory Toxicology, Therapeutic Drug Monitoring, Clinical Chemistry, Blood Banking/Transfusion Medicine, Therapeutic Apheresis

Selected Publications

Michael C. Milone and Bruce L. Levine: Powered and controlled T-cell production. Nature Biomedical Engineering 2: 148-150, 2018.

June Carl H, O'Connor Roderick S, Kawalekar Omkar U, Ghassemi Saba and Milone Michael C: CAR T cell immunotherapy for human cancer. Science 359(6382): 1361-1365, 2018.

Vijay Bhoj and Michael C. Milone: The pharmacology of T cell therapies. Molecular Therapy - Methods & Clinical Development 8: 210-221, 2018.

Trofe-Clark Jennifer, Brennan Daniel C, West-Thielke Patricia, Milone Michael C, Lim Mary Ann, Neubauer Robin, Nigro Vincenza, Bloom Roy D: Results of ASERTAA, a Randomized Prospective Crossover Pharmacogenetic Study of Immediate-Release Versus Extended-Release Tacrolimus in African American Kidney Transplant Recipients. American journal of kidney diseases : the official journal of the National Kidney Foundation Nov 2017.

Richman Sarah A, Nunez-Cruz Selene, Moghimi Babak, Li Lucy Z, Gershenson Zachary T, Mourelatos Zissimos, Barrett David M, Grupp Stephan A, Milone Michael C: High-Affinity GD2-Specific CAR T Cells Induce Fatal Encephalitis in a Preclinical Neuroblastoma Model. Cancer immunology research Nov 2017.

Kawalekar Omkar, June Carl H and Milone Michael C: Studying Immunoreceptor Signaling in Human T Cells Using Electroporation of In Vitro Transcribed mRNA. Methods Molecular Biology. Baldari, C and Dustin, M (eds.). Springer Nature, 1584: 443-450, Mar 2017 Notes: No issue number. doi: 10.1007/978-1-4939-6881-7_27.

Lambert Lester H, Goebrecht Geraldine K E, De Leo Sarah E, O'Connor Roddy S, Nunez-Cruz Selene, Li Tai-De, Yuan Jinglun, Milone Michael C, Kam Lance C: Improving T Cell Expansion with a Soft Touch. Nano letters 17(2): 821-826, Feb 2017.

Kawalekar Omkar U, O'Connor Roddy S, Fraietta Joseph A, Guo Lili, McGettigan Shannon E, Posey Avery D, Patel Prachi R, Guedan Sonia, Scholler John, Keith Brian, Snyder Nathaniel, Blair Ian, Milone Michael C*, June Carl H*: Distinct Signaling of Coreceptors Regulates Specific Metabolism Pathways and Impacts Memory Development in CAR T Cells. Immunity 44(2): 380-90, Feb 2016 Notes: *Co-senior author.

Bhoj Vijay G, Arhontoulis Dimitrios, Wertheim Gerald, Capobianchi James, Callahan Colleen A, Ellebrecht Christoph T, Obstfeld Amrom E, Lacey Simon F, Melenhorst Jan J, Nazimuddin Farzana, Hwang Wei-Ting, Maude Shannon L, Wasik Mariusz A, Bagg Adam, Schuster Stephen, Feldman Michael D, Porter David L, Grupp Stephen A, June Carl H, Milone Michael C: Persistence of long-lived plasma cells and humoral immunity in individuals responding to CD19-directed CAR T-cell therapy. Blood 128(3): 360-70, Jul 2016.

Ellebrecht Christoph T, Bhoj Vijay G, Nace Arben, Choi Eun Jung, Mao Xuming, Cho Michael Jeffrey, Di Zenzo Giovanni, Lanzavecchia Antonio, Seykora John T, Cotsarelis George, Milone Michael C*, Payne Aimee S*: Reengineering chimeric antigen receptor T cells for targeted therapy of autoimmune disease. Science 353(6295): 179-84, Jul 2016 Notes: *Contributed equally to the work.

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Last updated: 03/24/2018
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