Boerckel Laboratory

Annemarie Lang, Ph.D.

Post-doctoral Fellow

Home country: Germany

Education

Studies of Veterinary Medicine and Approbation (DVM) at Freie Universität Berlin, Germany 

Dr. med. vet. on Osteoarthritis Gene Therapy at Freie Universität Berlin, Germany

Ph.D. on Regenerative Medicine and Biomedical Sciences with a specific focus on fracture healing at Charité-Universitätsmedizin Berlin, Germany

Areas of Interest

Within my current research, I aim to uncover fundamental mechanisms that control endochondral ossification in fracture repair with a specific emphasis on the crosstalk between hypoxia and YAP/TAZ signaling by using state-of-the-art in vivo and in vitro models. Moreover, I aim for an active implementation of the 3R principle (Replace, Reduce and Refine) in science.

Annemarie joined McKay Orthopedic Research Lab in spring 2021 and is working towards completing her post-doc work. Annemarie came with her dog “Mephisto” and loves to explore Philadelphia with him.

Selected Publications

Lang, A., Helfermeier, S., Stefanowski, J., Kuppe, A., Sunkara, V., Pfeiffenberger, M., Wolter, A., Damerau, A., Hemmati-Sadeghi, S., Ringe, J., Haag, R., Hauser, A.-E., Löhning, M., Perka, C., Duda, G.N., Hoff, P., Schmidt-Bleek, K.*, Gaber, T.* & F. Buttgereit* (2020). HIF-stabilization prevents delayed fracture healing. Available as preprint on bioRxiv; ID: 182832. In submission.

Pfeiffenberger, M., Damerau, A., Ponomarev, I., Bucher, C.H., Chen, Y., Barnewitz, D., Thöne-Reineke, C., Hoff, P., Buttgereit, F., Gaber, T. & A. Lang (2021) Functional scaffold‐free bone equivalents induce osteogenic and angiogenic processes in a human in vitro fracture hematoma model. Journal of Bone Mineral Research. in press.

Damerau, A., Pfeiffenberger, M., Weber, M.-C., Burmester, G.-R., Buttgereit, F., Gaber, T.* & A. Lang* (2021). Mimicking cytokine-induced key features of arthritis in a human in vitro 3D osteochondral tissue model. International Journal of Molecular Sciences. Dec 24; 22(1), 128.

Sunkara, V., Heinz, G.A., Heinrich, F.F., Durek, P., Mobasheri, A., Mashreghi, M.-F.* & A. Lang* (2020). Combining segmental bulk- and single-cell RNA-sequencing to define the chondrocyte gene expression signature in the murine knee joint. Available as preprint on bioRxiv; ID: 148056. Osteoarthritis and Cartilage - accepted.

Weber, M.-C.*, Fischer, L.*, Damerau, A., Ponomarev, I., Pfeiffenberger, M., Gaber, T., Götschel, S., Lang, J., Röblitz, S., Buttgereit, F., Ehrig, R. & A. Lang (2020) Macroscale mesenchymal condensation to study cytokine-driven cellular and matrix-related changes during cartilage degradation. Biofabrication. Aug 10; 12(4):045016. 

Pfeiffenberger, M., Hoff, P., Thöne-Reineke, C., Buttgereit, F., Lang, A. & T. Gaber (2020) The in vitro human fracture hematoma model - a tool for preclinical drug testing. ALTEX. In press - https://doi.org/10.14573/altex.1910211

Stefanowski, J.*, Lang, A.*, Rauch, A., Aulich, L., Köhler, M., Buttgereit, F., Schmidt-Bleek, K., Niesner, R., Gaber, T. & A. Hauser (2019) Spatial Distribution of Macrophages during Callus Formation and Maturation Reveals Close Crosstalk between Macrophages and Newly Forming Vessels. Frontiers Immunology. Nov 26; 10:2588. 

Lang, A., Kirchner, M., Stefanowski, J., Durst, M., Weber, M.-C., Pfeiffenberger, M., Damerau, A., Hauser, A.E., Hoff, P., Duda, G.N., Buttgereit, F., Schmidt-Bleek, K.* & T. Gaber* (2019) Col I-based Scaffolds Negatively Impact Fracture Healing in a Mouse-Osteotomy-Model Although Used Routinely in Clinical Application. Acta Biomaterialia. Mar 1;86:171-184. 

Jirkof, P.*, Durst, M.*, Klopfleisch, R., Palme, R., Thöne-Reineke, C., Buttgereit, F., Schmidt-Bleek, K. & A. Lang (2019) Administration of Tramadol or Buprenorphine via the drinking water for post-operative analgesia in a mouse-osteotomy model. Scientific Reports. Jul 24;9(1):10749.

Pfeiffenberger, M.*, Bartsch, J.*, Hoff, P., Ponomarev, I., Barnewitz, D., Thöne-Reineke, C., Buttgereit, F., Gaber, T.* & A. Lang* (2019) Hypoxia and mesenchymal stromal cells as key drivers of initial fracture healing in an equine in vitro fracture hematoma model. PlosONE. Apr 4;14(4):e0214276. 

Hoff, P., Gaber, T., Strehl, C., Jakstadt, M., Hoff, H., Schmidt-Bleek, K., Lang, A., Röhner, E., Huscher, D., Matziolis, G., Burmester, G.R., Schmidmaier, G., Perka, C., Duda, G.N. & F. Buttgereit (2017) A Pronounced Inflammatory Activity Characterizes the Early Fracture Healing Phase in Immunologically Restricted Patients. International Journal of Molecular Sciences. Mar 8;18(3). pii: E583. 

Hoff, P., Gaber, T., Strehl, C., Schmidt-Bleek, K., Lang, A., Huscher, D., Burmester, G.R., Schmidmaier, G., Perka, C., Duda, G.N. & F. Buttgereit (2016) Immunological characterization of the early human fracture hematoma. Immunologic Research. 64(5-6): 1195-1206. 
 

Conference Abstracts and Presentations

Lang, A., Benn, A., Damerau, A., Boerckel, J.D., Kerckhofs, G., Zwijsen, A. First Steps Towards the BMP-SMAD1/5 Signaling Is Required for Adequate Coupling of Angiogenesis and Osteogenesis in Long Bones. ORS Congress 2021 - virtual Poster and Podium presentation - NIRA Award

Lang, A., Diesing, K., Damerau, A., Pfeiffenberger, M., Gaber, T., Buttgereit, F. First Steps Towards the Establishment of an In Vitro Trabecular Human Bone Model. ORS Congress 2021 - virtual Podium presentation - finalist for 3R Award of the ORS Preclinical Model Section & NIRA Awardee

Pfeiffenberger, M., Damerau, A., Hoff, P., Buttgereit, F., Gaber, T., Lang, A. A preclinical testing tool: The in vitro fracture gap model. ORS Congress 2021 - virtual Poster presentation 

Damerau, A., Pfeiffenberger, M., Buttgereit, F., Gaber, T., Lang, A. Three-dimensional human osteochondral tissue as a novel in vitro model of arthritis and preclinical drug screening platform. ORS Congress 2021 - virtual Poster presentation

Lang, A., Diesing, K., Damerau, A., Pfeiffenberger, M., Gaber, T., Buttgereit, F. Development of an In Vitro Trabecular Human Bone Model to Recapitulate Features of Glucocorticoid-induced Osteoporosis. EULAR Congress 2020 - virtual Poster presentation

Lang, A., Cai, L., Kuppe, A., Gaber, T., Guilak, F., Buttgereit, F., Rai, M.F. Characterization of a disease-inducible interleukin-4 gene therapy in murine articular chondrocytes. OARSI World Congress 2020 - https://doi.org/10.1016/j.joca.2020.02.797 – Abstract publication due to Covid19

Lang, A., Fischer, L., Weber, M.C., Damerau, A., Gaber, T., Götschel, S., Ehrig, R., Röblitz, S., Buttgereit, F. Combining in vitro and in silico modelling to simulate cartilage degradation during osteoarthritis. EUSAAT Congress 2019, Linz, Austria – Podium presentation

Lang, A., Fischer, L., Weber, M.-C., Gaber, T., Ehrig, R., Röblitz, S., Buttgereit, F. Combining in vitro simulation and in silico modelling towards a sophisticated human osteoarthritis model. OARSI World Congress 2019, Toronto, Canada – Poster presentation

Sunkara, V., Lang, A. Systems biology pipeline for precise extraction of the chondrocyte transcriptome in a whole tissue approach. OARSI World Congress 2019, Toronto, Canada – Poster presentation

Lang, A., Pfeiffenberger, M., Damerau, A., Buttgereit, F., Gaber, T. Mimicking the First Phase of Fracture Healing In Vitro. ORS Meeting 2019, Austin, Texas, US – Poster presentation

Lang, A., Gaber, T., Hoff, P., Schmidt-Bleek, K., Buttgereit, F. Application of HIF-Stabilizers Enhances Bone Regeneration in a Mouse Model of Compromised Conditions. ORS Meeting 2019, Austin, Texas, US – Poster presentation

Lang, A., Damerau, A., Weber, M.-C., Buttgereit, F., Gaber, T. First steps towards the in vitro simulation of an arthritic joint. OARSI World Congress 2018, Liverpool, UK – Poster presentation

Weber, M.-C., Ponomarev, I., Gaber, T., Buttgereit, F., Lang, A. Simulating osteoarthritis in vitro with human scaffold-free 3D cartilage transplants. OARSI World Congress 2018, Liverpool, UK – Poster presentation
 

Further Scientific Activities

  • Member of the OARSI Young Investigator Committee (Osteoarthritis Research Society International)
  • Board member of EUSAAT (European Society for Alternatives to Animal Testing)
  • Elected member of the Charité3R board - Representative EARA & EU3Rnet 
  • Editor-in-Chief for Laboratory Animals
  • Associate Guest Editor - OAC Open Special Themed Issue on Current advances in high throughput omics technologies for deep phenotyping in osteoarthritis
     

Awards/Fellowships

  • New Investigator Recognition Award 2021 (NIRA) - Orthopedic Research Society (ORS) 
  • DFG Research Fellowship for stay at UPenn 
  • Award for Alternative Methods from Berlin State
  • Add-on Fellowship for Systems Biology - Joachim Herz Foundation 
  • OARSI Belt and Road Scholarship funded by the Beijing Joint Care Foundation 
  • DAAD Postdoctoral Travel Award (2017, 2019) 
     

Collaborations at UPenn/CHOP

Riccardo Gottardi (CHOP) - An in vitro toolset to study endochondral ossification (3DEndOs) -Research Grant by Alternatives Research and Development Foundation (ARDF) - PI: Joel D. Boerckel

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