Camera-based Confocals

These systems are optimized for rapid confocal imaging of live or fixed specimens.

Room 1175B BRB II/III

Features

  • Olympus IX71 inverted microscope
  • Visitech VT iSIM scan head for real-time 2x spatial resolution enhancement in x, y, and z
  • Hamamatsu ORCA Flash 4.0 sCMOS camera
  • Excitation laser lines: 405, 442, 488, 514, 561, 640 nm
  • Roper iLas2 ablation (355 nm) and FRAP/photoactivation (405 nm) system.
  • Motorized xy stage for multi-point acquisition
  • LCI Chamlide stagetop incubation system for live mammalian cell imaging
  • MetaMorph acquisition software
  • Check out dye compatibility with this system on our FPBase page

Best suited for: fast high-resolution confocal imaging of live or fixed samples

Room 1175D BRB II/III

Features:spinning disk confocal

  • Olympus IX81 inverted microscope
  • Excitation laser lines: 405, 488, 561 (for Hoechst, GFP, RFP)
  • Andor iXon3 EMCCD camera
  • Motorized x-y stage for multi-point acquisition or tile scanning
  • Roper iLas2 FRAP/photoactivation system (405 nm laser)
  • Okolab stagetop incubation chamber
  • MetaMorph acquisition software
  • Check out dye compatibility with this system on our FPBase page

Best suited for: fast confocal imaging of sensitive, live samples; FRAP, photoactivation, photoconversion in live cells or organisms