Camera-based Confocals
These systems are optimized for rapid confocal imaging of live or fixed specimens.
Room 1175B BRB II/III
Features
- Olympus IX71 inverted microscope
- Visitech VT iSIM scan head for real-time 2x spatial resolution enhancement in x, y, and z
- Hamamatsu ORCA Quest qCMOS camera
- Excitation laser lines: 405, 488, 561, 640 nm
- Gataca Systems iLas2 ablation (355 nm) and FRAP/photoactivation (405 nm) system.
- Motorized xy stage for multi-point acquisition
- LCI Chamlide stagetop incubation system for live mammalian cell imaging
- MetaMorph acquisition software
- Check out dye compatibility with this system on our FPBase page
- For users: a cheat sheet showing main MetaMorph software features
Best suited for: fast high-resolution confocal imaging of live or fixed samples
Room 1175D BRB II/III
Features:
- Olympus IX81 inverted microscope
- Excitation laser lines: 405, 488, 561 (for Hoechst, GFP, RFP)
- Andor iXon3 EMCCD camera
- Motorized x-y stage for multi-point acquisition or tile scanning
- Gataca Systems iLas2 FRAP/photoactivation system (405 nm laser)
- Okolab stagetop incubation chamber
- MetaMorph acquisition software
- Check out dye compatibility with this system on our FPBase page
Best suited for: fast confocal imaging of sensitive, live samples; FRAP, photoactivation, photoconversion in live cells or organisms
For new users of this microscope: a handy 1-page guide to MetaMorph functions
Room 1175D BRB II/III
Features:
Olympus IX83 inverted microscope
objectives: 10x/0.4, 20/0.8, 40x/0.95 (dry); 60x/1.3 NA (silicone); 100x/1.45 NA (oil)
Excitation laser lines: 405, 445, 488, 520, 555, 630 (check out dye compatibility on our FPBase page)
TWO pco.edge 4.2 bi sCMOS cameras for simultaneous green/red imaging
motorized x-y stage for multi-point acquisition or tile scans
Visitron FRAP/ablation system with 405 nm laser for FRAP/PA and pulsed 355 nm laser for ablation
VisiView software (by Visitron) for acquisition
Okolab incubation enclosure for live cell imaging