Laser-scanning Confocal Microscopes

Room 1175A BRB

Features:Zeiss LSM 880

  • Zeiss Axio Observer inverted microscope
  • ZEN v2.3 software for acquisition
  • Excitation laser lines: 405, 458, 488, 514, 561, 633 nm
  • Objectives: 10x/0.3 dry, 20x/0.8 dry, 25x/0.8 Imm, 40x/1.2 W, and 63x/1.4 Oil
  • Three spectral detection channels: 2 metal alkali PMT + 1 GaAsP detector
  • Airyscan module with FAST option for enhanced resolution, speed, and sensitivity
  • Environmental chamber and motorized x-y stage for live imaging and tiling applications

Best suited for: confocal imaging of fixed or live samples, including those requiring incubation; FRAP, photoactivation, or photoconversion in live cells or organisms; Airyscan module provides 1.7x increase in resolution; FAST module provides 4x increase in speed.

Room 1-126C Smilow

Features Zeiss LSM 710

  • Zeiss AxioObserver inverted microscope
  • ZEN 2012 software for acquisition
  • Excitation laser lines: 405, 458, 488, 514, 561, 594, 633 nm
  • Objectives: 10x/0.45 dry; 20x/0.8 dry; 25x/0.8 Imm; 40x/1.1 W; 63x/1.4 Oil
  • Two metal alkali PMTs and one 32-channel linear array PMT
  • Stagetop environmental chamber and motorized x-y stage for live imaging and tiling applications

Best suited for: confocal imaging of fixed or live samples, including those requiring incubation; FRAP, photoactivation, or photoconversion in live cells or organisms; FRET; spectral characterization and linear unmixing of unorthodox dye combinations

Room 1-126D Smilow

Features: Leica TCS SP8

  • DMI 6000 inverted microscope
  • LAS X software for acquisition
  • Excitation laser lines: 405, 488, 552, 638 nm
  • Objectives: 10x/0.4 dry; 20x/0.75 dry; 25x/0.95 W; 40x/1.10 W; 63x/1.4 Oil
  • Three spectral detection channels: 2 PMTs and 1 HyD detector
  • Tokai-Hit stagetop incubation chamber
  • FRAP and FRET wizards
  • Motorized x-y stage for and tiling applications

Best suited for: confocal imaging of fixed or live samples, including those requiring incubation; FRAP, photoactivation, or photoconversion in live cells or organisms; FRET

Room B30A Anatomy-Chemistry

Features: Leica STED

  • Leica DMI 6000 inverted microscope
  • Pulsed white-light laser for tunable excitation from 470 to 670 nm
  • CW laser lines for conventional confocal imaging: 405, 458, 488, 514 nm
  • Objectives: 10x/0.4 dry; 20x/0.75 Imm; 40x/1.3 Oil; 63x/1.4 Oil; 100x/1.4 Oil
  • High-speed resonant scanner for live-cell imaging
  • 3 HyD and 2 PMT detectors
  • Two depletion lasers (592 and 660 nm) for super-resolution imaging of green and red dyes
  • Tokai Hit stagetop incubator
  • Motorized xy stage
  • LAS X software for acquisition; Huygens Professional software for deconvolution

Room 514A CRB

Features:

  • Visible laser lines (all diode or DPSS lasers): 405, 488, 514, 552, 638
  • Coherent Chameleon Vision II + MPX wavelength extender for tunable excitation from 740 — 1300 nm
  • Leica DM 6000 upright microscope
  • Objectives: 10x/0.3 dry; 20x/0.75 Imm; 40x/1.3Oil; 63x/1.4 Oil; 25x/1.0 W motCORR available upon request
  • High-speed resonant scanner for live-cell imaging
  • 2 HyD and 3 PMT  confocal detectors
  • HyD and PMT dual NDDs for reflected light (fluorescence or SHG)
  • PMT NDD for transmitted light (SHG)
  • Motorized xy stage
  • LAS X software for acquisition