- Information for new users
- How to get trained
Training for light microscopes in the CDB Microscopy Core
All new users of our core's light microscopes must follow the same training procedure, regardless of background experience. If you are unsure as to which type of microscope is best for your experiment, contact Andrea Stout or Jasmine Zhao to schedule a consultation.
The next training lecture will take place via IN PERSON on Wednesday December 1, 2021, 1:00 — 2:30 pm in room 1101 BRB. Email Andrea to be added to the list; a reminder email will be sent out the last business day before (November 30).
Attendance at the monthly pre-training lecture is recommended but not required for our widefield microscopes. Click the plus sign for detailed instructions on how to request hands-on training.
IMPORTANT: Do NOT submit an iLab confocal training request if you want to be trained on a widefield microscope.
Arrange a hands-on training session the same way you would arrange an assisted session:
- Set up your iLab account
- Email Andrea or Jasmine to discuss your experiment and arrange a time to meet at the microscope. Be prepared to bring a sample similar to those you“ll be using for your project.
- The following microscopes fall into this category: Leica widefield and EVOS FL Auto in Smilow, and Zeiss Observer 7 in BRB.
- Training involves (a) watching a series of video clips showing proper operation of the microscope; (b) a remote desktop session where core staff explains the acquisition software while the trainee shares the screen but is not present; c) an in-person session with proper social distancing where the trainee operates the system while staff watch and answer questions. Time for training (remote desktop + in-person training sessions) is charged at the assisted rate for that instrument.
Attendance at the pre-training lecture is mandatory for all who wish to do hands-on training on a confocal. Click the plus sign for more detailed instructions.
IMPORTANT: Do NOT submit an iLab confocal training request! When you are ready to be trained, email Andrea or Jasmine.
Please follow these instructions when requesting confocal training
- Email Andrea with a request to be added to the list for the next training lecture. This is also a good time to describe your plans for using our confocals.
- Set up your iLab account.
- Recommended (but not required) for labs new to our core: request an assisted session with Andrea or Jasmine to see if your samples are compatible with or benefit from confocal imaging.
- Training involves (a) watching a series of video clips showing proper operation of the microscope; (b) a remote desktop session where core staff explains the acquisition software while the trainee shares the screen but is not present; c) an in-person session with proper social distancing where the trainee operates the system while staff watch and answer questions.
- Trainees must schedule their first post-training session within 3 weeks of in-person hands-on training in order to gain access to the iLab scheduler.
A flat fee of $300 ($489 for corporate customers) is charged for the first hands-on confocal training session. This fee includes the training session itself, the first post-training session, and 2 additional hours of unassisted confocal usage provided that this takes place within 1 month of the hands-on training session.
Users who request hands-on training on a second confocal will be charged the hourly rate for assisted time posted here.
Attendance at the pre-training lecture is mandatory for all who wish to do hands-on training on one of these systems. Click the plus sign for more information and note the extra requirements.
Please follow these instructions when requesting training on one of the above systems
- Email Andrea with a request to be added to the list for the next training lecture if you haven't already attended it.
- Set up your iLab account if you haven't done that yet.
- Set up a meeting with Andrea to discuss (a) sample preparation requirements for your chosen modality and (b) discuss technical details of your chosen modality that differ from conventional widefield or confocal.
- Arrange with Andrea to do an assisted session prior to hands-on training. This is mandatory: you will not be trained during this session, but you will see what's involved and will learn what data management strategies you should get in place and what (if any) sample prep modifications you should implement before you begin using the system.
- After your assisted session, arrange a separate hands-on training session with Andrea. After completion of this, you may reserve the microscope to work independently.