Specialized Microscopy SystemsThese non-traditional microscopes require special sample preparation and more extensive training than our confocals
Zeiss Lightsheet Z.1
Room B30 Anatomy-Chemistry
- Dual side illumination with 405, 488, 561, or 638 nm lasers
- Dual sCMOS cameras for simultaneous imaging of two channels
- 5x and 20x optics and chambers for n = 1.33 (aqueous) and n = 1.45 (CLARITY) specimens
- ZEN 2014LS software for acquisition
- Arivis Vision4D software for post-acquisition stitching and rendering of large data sets
Best suited for: Very fast multi-dimensional imaging of transparent live or cleared specimens. Live specimens are typically mounted in an agarose column and suspended in water or other aqueous medium. Cleared specimens must be glued to a support and suspended in a n = 1.45 solution such as FocusClear or RIMs.
Bruker Vutara 352 super-resolution microscope
Room B38 Anatomy-Chemistry
Single-molecule localization (PALM/STORM) microscopy for 20 nm lateral, 50 nm axial resolution.
405 nm laser for activation; 488, 560, and 640 nm lasers for imaging
Olympus 60x/1.2 NA W objective; Hamamatsu ORCA Flash 4.0 v3 sCMOS camera
Best suited for: 1- or 2-color super-resolution imaging of thin aqueous samples using recommended dyes or photoconvertable FPs.
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