Cell and Developmental Biology Microscopy Core

Specialized Microscopy Systems

These non-traditional microscopes require special sample preparation and more extensive training than our confocals

Zeiss Lightsheet Z.1

Room B30 Anatomy-Chemistry

Features

  • Dual side illumination with 405, 488, 561, or 638 nm lasers

     

     

  • Dual sCMOS cameras for simultaneous imaging of two channels
  • 5x and 20x optics and chambers for n = 1.33 (aqueous) and n = 1.45 (CLARITY) specimens
  • ZEN 2014LS software for acquisition
  • Arivis  Vision4D software for post-acquisition stitching and rendering of large data sets

Best suited for: Very fast multi-dimensional imaging of transparent live or cleared specimens. Live specimens are typically mounted in an agarose column and suspended in water or other aqueous medium. Cleared specimens must be glued to a support and suspended in a n = 1.45 solution such as FocusClear or RIMs.

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Bruker Vutara 352 super-resolution microscope

Room B38 Anatomy-Chemistry

Features:Vutara352

Single-molecule localization (PALM/STORM) microscopy for 20 nm lateral, 50 nm axial resolution. 

405 nm laser for activation; 488, 560, and 640 nm lasers for imaging

Olympus 60x/1.2 NA W objective; Hamamatsu ORCA Flash 4.0 v3 sCMOS camera

Best suited for: 1- or 2-color super-resolution imaging of thin aqueous samples using recommended dyes or photoconvertable FPs.

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