Specialized Systems
Our Zeiss Lightsheet Z.1 is considered a specialized system due to its unique requirements for sample preparation.
Room B30 Anatomy-Chemistry
Features
- 5x/0.16 NA and 20x/1.0 NA for RI = 1.33 (water) and RI = 1.45 (CLARITY/CUBIC)
- Laser lines: 405 nm, 488 nm, 561 nm, and 638 nm
- Peltier heating for live imaging of aqueous specimens
Best suited for:
- Live or fixed imaging of transparent, aqueous samples that can be suspended in agarose such as zebrafish or organoids
- Fixed imaging of CLARITY or CUBIC-cleared tissues less than 1 cm in diameter
Before you plan to use this system, watch the following introductory videos on sample preparation:
Not every sample is compatible with the above two methods, so you may need to get creative and consult other resources for ideas. This Nature Protocols paper is based on experiments done using our Core's system and contains many useful methods for sample prep, imaging, and image analysis.