Widefield Microscopes

Room 1175C BRB

Features:Zeiss Observer 7

  • Zeiss Axio Observer 7 inverted microscope

  • Dual cameras: Zeiss Axiocam 702 monochrome CMOS camera and Zeiss Axiocam 503 color CCD camera

  • Colibri 7 LED light source (see our FPbase page for excitation wavelengths)

  • Independent emission filter wheel for customizable fluorescence settings (see our FPbase page  to view standard filter combinations)

  • 5x, 10x, 20x, 40x, and 100x objectives

  • Environmental chamber surrounding entire microscope for temperature and CO2 control

  • ZEN Blue software for acquisition and deconvolution

Best suited for: rapid, high-resolution tile scanning of large areas; high-resolution 3D imaging and deconvolution of thin (< 15 microns thick) samples; long time-lapse imaging using transmitted light or epifluorescence. High resolution color imaging of chromogenic stains

Room 1-127 SCTR

FeaturesEVOS FL Auto 2

  • Inverted platform holds a variety of vessels including multi-well plates (glass or plastic bottom)
  • Dual cameras allow both color and monochrome imaging
  • long-working distance objectives: 4X, 10X, 20X, 40X
  • LED light cubes for DAPI, GFP, RFP, Texas Red, Cy5, and Cy7 (click here for details)
  • environmental chamber with gas mixer for live imaging under normal or hypoxic conditions
  • fully automated stage for tile scanning and multipoint acquisition
  • easy-to-use EVOS software

Best suited for: Single images or tile scans of fixed tissue stained with chromogenic dyes or fixed cells stained with fluorescent markers; live imaging of cells or organisms; hypoxia experiments

Room 1-126A SCTR

Features:Leica wide field

  • Leica DM6000 motorized upright microscope
  • Photometrics HQ2 high resolution monochrome CCD camera
  • LAS AF software for acquisition
  • AutoQuant deconvolution package (within LAS)
  • Leica filter sets for DAPI, GFP, Cy3, and Cy5 (spectra here or at FPBase)

Best suited for: high-resolution widefield deconvolution microscopy of thin (<15 microns), fixed samples; widefield epifluorescence imaging of fixed samples.