Widefield Microscopes
Room 1175C BRB
Features:
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Zeiss Axio Observer 7 inverted microscope
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Dual cameras: Zeiss Axiocam 702 monochrome CMOS camera and Zeiss Axiocam 503 color CCD camera
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Colibri 7 LED light source (see our FPbase page for excitation wavelengths)
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Independent emission filter wheel for customizable fluorescence settings (see our FPbase page to view standard filter combinations)
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5x, 10x, 20x, 40x, and 100x objectives
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Environmental chamber surrounding entire microscope for temperature and CO2 control
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ZEN Blue software for acquisition and deconvolution
Best suited for: rapid, high-resolution tile scanning of large areas; high-resolution 3D imaging and deconvolution of thin (< 15 microns thick) samples; long time-lapse imaging using transmitted light or epifluorescence. High resolution color imaging of chromogenic stains
Room 1-127 SCTR
Features
- Inverted platform holds a variety of vessels including multi-well plates (glass or plastic bottom)
- Dual cameras allow both color and monochrome imaging
- long-working distance objectives: 4X, 10X, 20X, 40X
- LED light cubes for DAPI, GFP, RFP, Texas Red, Cy5, and Cy7 (click here for details)
- environmental chamber with gas mixer for live imaging under normal or hypoxic conditions
- fully automated stage for tile scanning and multipoint acquisition
- easy-to-use EVOS software
Best suited for: Single images or tile scans of fixed tissue stained with chromogenic dyes or fixed cells stained with fluorescent markers; live imaging of cells or organisms; hypoxia experiments
Room 1-126A SCTR
Features:
- Leica DM6000 motorized upright microscope
- Photometrics HQ2 high resolution monochrome CCD camera
- LAS AF software for acquisition
- AutoQuant deconvolution package (within LAS)
- Leica filter sets for DAPI, GFP, Cy3, and Cy5 (spectra here or at FPBase)
Best suited for: high-resolution widefield deconvolution microscopy of thin (<15 microns), fixed samples; widefield epifluorescence imaging of fixed samples.