Histology Core Rates

The PCMD Histology Core is pleased to offer competitive rates for both full-service projects and use of self-service equipment. In most cases we are able to provide additional subsidies for full PCMD members. Please use the links below to view current fees for full and affiliate PCMD members. The core offers an expedited service for an additional fee (available upon request).

NEW Offering! Hybridization Chain Reaction

Fluorescence In Situ Hybridization, HCR-FISH

The McKay Histology Core is pleased to announce the addition of RNA Fluorescence In Situ Hybridization using hybridization chain reaction (HCR-FISH, www.molecularinstruments.com) as a full service offering. The service includes HCR-RNA FISH labelling performed by the core technical director, and all the necessary buffers and amplification hairpin probes needed to label your samples in three wavelengths — B1 - 647, B2 - 488, and B3 - 546 hairpins. Users provide the core with slides mounted with OCT (regular or cryofilm) or paraffin sections, and the HCR-RNA Molecular Instruments probe for your gene of interest. The core can provide advice on probe selection and design. The cost of performing RNA FISH is $250 + $7/section for one experiment to core full members and $300 + $8.40/section to affiliate members. The core is also able to provide additional processing services, including RNase-free fixation, embedding and sectioning, for a reasonable fee if the user provides freshly isolated samples.

Also available at the Histology core:

The core also has three "new" pieces of equipment available to self-service users:

  • A NEW 'Dental' X-ray camera/machine that can be used for small animal procedures such as bone fracture experiments, assessing bone calcification during fixation and decalcification studies, as well as any other research needs involving X-ray imaging.
  • A low profile-blade paraffin microtome
  • Hybridization oven for RNAScope and HCR-FISH experiments.

Please contact the Core Technical Director, Edgardo J. Arroyo (arroyoe@pennmedicine.upenn.edu) for further information on HCR-FISH, or to arrange access and equipment training.