Hydrogen-Deuterium Exchange Mass Spectrometry

Protein hydrogen exchange rates measure the stability and dynamics of backbone hydrogen bonded structure. After incubation in D2O we measure deuterium content of peptide fragments by mass spectrometry. The facility has two mass spectrometers each equipped with custom LC systems, a Thermo Q-Exactive and a Thermo Exactive plus EMR.

Recent HX publications:

Contact: Leland Mayne | mayne@pennmedicine.upenn.edu
Location: 1010 Stellar-Chance Laboratory