Beta-Galactosidase & Chloramphenicol Acetyltransferase Assays

Beta-Galactosidase Staining

Chemiluminescent B-gal Detection

BOSC23 Transfection and Infection of NIH 3T3 Cells

Tet Vector Transfection of BOSC23 cells and NIH3T3 cell Infection

Cloning and Expression Vectors

Cloning in Alkaline Phosphatase-Treated Vector

Colony Hybridization

DAPI Staining of Nucleus


DNA Fragment Purification from Agarose Gel

DNAse I Footprinting

Phage Lambda DNA Preparation

Purification by CNBr Coupling Protocol

High Efficiency Plasmid Recovery

Molecular Biology Protocols

P32 Labeling of GST Fusion Proteins

Molecular Cloning Protocols

Preparation of Competent Cells

Replicaplating Colonies onto Nitrocellulose Filters

Representational Difference Analysis