Protocols
The Robertson Lab All Protocols List- Alkaline Phosphatase
- Animal and Cell Culture Procedures
- BACmid Recombineering Protocols
- Bacteria Growth Transformation
- Beta-Galactosidase Assays
- BOSC23 Transfection and Infection of NIH 3T3 Cells
- BrdU Staining FACS Protocols
- Calcium Phosphate with BBS Transfection
- Cell Culture Protocols
- Chromatin Immunoprecipitation Assay
- Cloning and Expression Vectors
- CsCl Purification of Plasmid DNA
- DAPI Staining of Nucleus & Dialysis
- DNA Protocols
- ELISA & EMSA Assays
- Flow Cytometry & Fluorescence in Situ Hybridization
- Genomic DNA Isolation System
- Hypoxia & Metabolism
- Immuno Related Assays
- In Situ Protocols
- Isolation Stem Cell
- Instruction Protocols
- In vitro Assays
- Kinase Assays
- Lab Guidelines
- Liposome Transfection & Luciferase Protocols
- Matrigel Assay
- Membrane Hybridization Protocols
- Microarray Pathochip Protocols
- Molecular Biology Protocols
- Nuclear & Oligo Protocols
- pFLASH Phagemids & Protein Protocols
- ptTS-Neo Vector Information & Recipes for Solutions
- Replicaplating Colonies onto Nitrocellulose Filters & Representational Difference Analysis
- RNA Related Protocols
- Sequencing & Serum Related Protocols
- Southern Blotting Protocols & SYBR Green Information
- Taq Polymerase Preparation
- Transfection Protocols & Transient Transduction with Adenovirus Poly Lysine (AdpL)
- Vent DNA Polymerases & Virus Preparation Protocols
- Western Blotting Protocols
- Yeast Protocols
Animal and Cell Culture Procedures
Freezing Cells
- A Highly Efficient Recombineering-Based Method for Generating Conditional Knockout Mutations
- BAC DNA Mini-Preparation
- BAC Recombineering Vectors
- BAC Recombineering Protocol
- BAC Recombineering Protocol #1
- BAC Recombineering Protocol #2
- BAC Recombineering Protocol #3
- BAC Recombineering Protocol #4
- BAC d E3c
- DNA Max-Preparation Protocol
- High-Efficiency Counterselection Recombineering for Site-Directed Mutagenesis in Bacterial Artificial
- J. Virol.-2002-Zhou-6185-96
- NEBlot Kit Instruction Manual Preparation of High Specific Activity DNA Probes
- pEL04 Map
- pGalK Map
- pTamp Map
- pIGCN21 Map
- PL253 Map
- PL451 Map
- PL452 Map
- Preparing Solutions for BAC DNA Preparation
- QIAGEN Large-Construct Kit Handbook
- Recombineering a Homologous Recombination-Based
- Simple and Highly Efficient BAC Recombineering Using
- Strains
Bacteria Growth Transformation. Maintenance and Bacteriophase
- 2 x YT Medium, M9 Minimal Medium
- Antibiotic Solutions
- Diluent
- LB Medium, NZCYM Medium, NZYM Medium, NZM Medium
- Media with Agar or Agarose
- Solutions for Working with Bacteriophage
- Storage Media, Stab Cultures, Bacterial Cultures Growing in Liquid Media and Agar Plates Terrific Broth, SOB Medium, SOC Medium
- Terrific Broth, SOB Medium, SOC Medium
T4 Polynucleotide Kinase Assays
Beta-Galactosidase & Chloramphenicol Acetyltransferase Assays
Beta-Galactosidase Staining
Chemiluminescent B-gal Detection
BOSC23 Transfection and Infection of NIH 3T3 Cells
Tet Vector Transfection of BOSC23 cells and NIH3T3 cell Infection
Cell Culture Protocols
- Cell Lysis for PCR Analysis
- Collecting and Transfecting Cells
- Epithelial Cell Lines
- FICOLL
- GPT selection
- HVS Transformation of PBMCs
- Lymphocyte Isloation by Ficoll Gradient Centrifugation
- Maintaining 293GP Clones in Culture
- Mammalian Cell Selection by Panning
- Mycophenolic Acid Selection
- Preparation of Cell Cultures and Vaccinia Virus Stocks
- Preparation of Insect Cell Cultures and Baculoviral Stocks
- Primary Lymphocyte Electroporation
- Protein Expression in Insect Cells Using Baculoviral Vectors Overview
- Spin Infections
- Transformation Efficiency Assay
Competent Cells
E. Coli Transformation
Cloning and Expression Vectors
Cloning in Alkaline Phosphatase-Treated Vector
Colony Hybridization
DNA Fragment Purification from Agarose Gel
- cDNA Amplification Kit
- CHART RNA
- DNA Ladder Protocol
- DNA Max-Preparation Protocol
- DNA Mini-Preparation Protocol V1
- DNA Maxi-Preparation. V1 9.06
- Garbella Cell Gel Assay
- Gel Shift Assay
- Hirt supernatent DNA Preparation
- Mini-Preparation for Plasmid Extraction
- Mini Preparation Protocol V2
- Nuclei Paraffin DNA
- Oligo DNA
- Optimization of DNA-RNA Extraction from FFPE paper
- Phenol-Based Methods for Isolating DNA from Agarose Gels
- Preparation Crude DNA for PCR- Preparation of Phage Lamda DNA
- Protocols for the in Situ PCR-Amplification and Detection of mRNA and DNA Sequences
- Rapid Separation of Protein-Bound DNA
- S35 DNA Sequencing
- Salmon Sperm DNA
- Simultaneous Purification of Genomic DNA and Total RNA (Supplementary)
- Trizol Reagent
DNAse I Footprinting
- DNAse I protocol for footprinting ToxR membranes
- Optimization and troubleshooting DNase I footprinting reactions
- P32 Labeling of Large DNA Fragments
Phage Lambda DNA Preparation
Purification by CNBr Coupling Protocol
- Analysis of DNA-Protein Interactions Using Proteins Synthesized In Vitro from Cloned Genes
- CNBr-Activated Sepharose 4 Fast Flow
- CNBr-Activated Sepharose 4B
- Detection, Purification, and Characterization of cDNA Clones Encoding DNA-Binding Proteins
- DNA-Protein Interactions
- DNAse I Footprint Analysis of DNA-Protein Binding
- Methylation Interference Assay for Analysis of DNA-Protein Interactions
- Motility Shift DNA-binding Assay Using Gel Electrophoresis
- Preparation of Nuclear and Cytoplasmic Extracts from Mammalian Cells
- Purification by CNBr Coupling
- Purification of DNA-Binding Proteins Using Biotin. Streptavidin Affinity Systems
- Purification of Sequence-Specific DNA-Binding Proteins by Affinity Chromatography
- Rapid Separation of Protein-bound DNA From Free DNA Using Nitrocellulose Filters
- Types of Chromatography
- UV-Crosslinking of Proteins to Nucleic Acids
High Efficiency Plasmid Recovery
ELISA Assays
- DNA Quality for Transfection
- Electrocompetent E Coli Cells
- ELISA with HRP, ABTS and Acid Stop
- ELISA with HRP and TMB
- Superfect Transfection
- Transfection Reagent
EMSA Electro-Mobility Shift Assays
Flow Cytometry
- Apoptosis Detection with Permeable Cells
- Detection of Apoptosis in Unfixed Samples with Hoechst 33342
- DFCI Flow Cytometry Facility General Info
- DNA Staining with Chromomycin A3
- Hoechst 33342 Staining of Viable Cells
- Indo 1 Analysis for Calcium Determination
- Propidium Iodide Staining for Cell Cycle Determination
- Propidium Iodide Staining for Dead Cell Exclusion
Fluorescence in Situ Hybridization
- BAC Fluorescence ISH Single Color
- FISH Slides
- FISH with TSA
- Fluorescence In Situ Hybridization
- In Situ Detection of RNA and DNA
- In Situ DNA Hybridization Products
- Introduction of Micromax Human cDNA Microarray System I
- One color FISH - Nick translation
- Standard Protocol
- Summary of TSA-Direct ISH
- TSA and In Situ Hybridization
- TSA Diagrams
- TSA-Direct Kit Components
- TSA-Direct Kits
Immuno Assays
- Western Immunoblot Protocol
- Double Staining Immunfluorescence GBP-1 CD31
- Double Staining Ki-67-CD31
- ImmunoFISH
- Preparation of Human Sera for EBV Latent Antigen Detection
Immunoaffinity Purification of Antibodies by GST Fusion Proteins
Immunofluorescence
- Immunofluorescence Protocol
- Immunofluorescence Staining
- Immunofluorescent Staining and Flow Cytometry Procedure
Immunoperoxidase Staining
Immunoprecipitation
- Immunoblotting Procedure
- Immunoprecipitation Protocol
- Immunoprecipitation with Staphylococcus Aureus Cells
- Immunoprecipitation
- ImmunoPure and UltraLink Proteins L.A.G.AG
- Immunoprecipitation Experiment with EBNA3C and Prothymosin
- Monoclonal Antibodies to DNA Tumor Viruses
- Preparation of Protein A Beads
Western Blotting ECL Detection
In Situ Amplification and Hybridization
In Situ Hybridization of Bacteriophage Plaques
- Hybridization to Nitrocellulose Filters with Bacteriophage Plaques Replicas
- Immobilization of Bacteriophage Plaques
- In Situ Hybridization of Bacteriophage Plaques
In Situ PCR
Instruction Protocols for sense basic Molecular Biology
- A Guide to the Preparation and Use of Buffer
- cDNA Amplification Kit
- Core Footprinting System
- Glogos Autorad Markers
- Instructions for Using the Sonic Dismembrator
- LacSwitch II Inducible Mammalian Expression System
- Living Colors Fluorescent Protein Protocols
- Matchmaker Two-Hybrid System
- pGEM-T and pGEM-T Easy Vector Systems
- Primer Extension System - AMV Reverse Transcriptase
- Push Column Beta Shield Device and Nuctrap Probe
- Stratagene Random Primer Labeling Kit
- Taq Extender PCR Additive
- Yeast Protocols Handbook
In vitro Assays
- In vitro Processivity Assay
- In vitro Translation
- In vitro binding Assay Protocol
- In vitro kinase Assay Protocol
- Ubiquitylation Assay
- HAT Assay
- IP HAT Assay
- Transcription Initiation by RNA Polymerase II
Histone Deacetylase Assay
- Anti-Histone H1 Protocols
- Histone deacetylase assay kit
- Histone H4 Peptide Radiolabeling
- HDAC Protocol
TnT Protocols
Liposome Transfection
Luciferase
- Analysis of Genomic DNA by Southern Hybridization
- Buffers and Solutions
- Gene Screen Plus Hybridization Transfer Membrane Manual Table of Contents
- Hybridization to Nitrocellulose Filters Containing Bacteriophage Plaques or Bacterial Colonies
- Isolation of DNA from Mammalian Cells
- Random Priming BMB Kit
- Sieving Agarose Gel Electrophoresis
- Southern Blotting and Hybridization
- Southern Transfer Membrane Hybridization
Microarray Pathochip Protocols
- 2.5% Gel Electrophoresis
- Amplification
- Amplification-Step C
- DNA purification
- DNA-RNA Extraction and Purification
- Hybridization
- Library Synthesis Reaction
- Procedures for Pathochip Microarray Experiment for Tumor FFPE samples
- Purification of Amplified DNA-QIAquick PCR Purification Kit
- RNA Purification
- Sample Labeling. Step 1 Fluorescent Labeling of gDNA
- Sample Labeling Procedure. Step 2 Clean-up Labeled samples
- Vacuum Centrifuge Operation
- Washing Microarray Scanning Steps
Data Analysis Using Partek Genomic Suite
Molecular Biology Protocols
- Doublestaining Ki-67 CD31 mit EnVision
- Factors that Influence
- FICOLL
- Gelatin Zymography Protocol
- Gst Pulldown for Proteomic Study
- Harvesting Cells for Luciferase Assay
- High-Risk Behaviors Among Adult Men & Women In Botswana: Implications for HIV-AIDS Prevention Efforts
- Knowledge HIV UB Students
- Lysis Buffer Preparation of LMP2 Membrane Extracts
- Montaner CITI
- Packaging Extract Protocol II
- Passage of C-terminal XbaI Linker Mutant Virus from E1 C64 No 22 LCL
- Phase Lock Gel Manual
- Preparation of Vectors Treated with Alkaline Phosphatase
- Pulse Chase Assay
- Refractive Index Reference for CsCl
- Ribonuclease Protection Assay
- Shift Western Protocol
- Staph Preparation
- Stratagene Gigapack Gold Packaging Extract
- Sucrose Gradient Centrifugation
- Western Blot of Protein Expressed by Yeast
P32 Labeling of GST Fusion Proteins
Molecular Cloning Protocols
- Agar, Agarose and Concentrated Media
- Antibiotics
- Autoradiography
- Buffers for Restriction Endonuclease Digestion
- Cell Counts with a Hemacytometer
- Chromatography Through Sephadex G-50
- Common Electrophoretic Buffers
- Commonly Used Gel-loading Buffers
- Concentration of Nucleic Acids
- Determination of Viability by Trypan Blue Exclusion
- Drying Down 32P-labeled Nucleotides from Mixtures of Ethanol and Water
- Enzymes
- Glassware and Plasticware
- Liquid Media
- Mutation Detection by Cleavase Fragment Length Analysis
- Measurement of Radioactivity in Nucleic Acids
- Northern Blotting
- Preparation of Buffers and Solutions
- Preparation of Dialysis Tubing
- Preparation of Multimers of Plasmids as Molecular Weight Markers
- Preparation of Organic Reagents
- Preparation of Stock Solutions
- Proteolytic Enzymes
- Protocol for Sequencing by the Maxam-Gilbert Technique
- Purification of Nucleic Acids
- Quantification of DNA and RNA
- Solutions for Working with Bacteriophage Lambda
- Solutions Biochemical Techniques
Preparation of Competent Cells
pFLASH Phagemids Protocols
Protein Expression Protocols
- Amplification Using CHO Cell Expression Vectors
- Characterization of Recombinant Vaccinia Viruses and Their Products
- Generation of Recombinant Baculoviruses and Analysis of the Expressed Proteins
- Generation of Recombinant Vaccinia Viruses
- Overview of Protein Expression in Mammalian Cells
- Overview of the Vaccinia Virus Expression System
- Purification and Transfection of Baculoviral DNA for Generating Recombinant Viruses
- Purification of Untagged Ubiquitin from Bacterial Cells
- Transient Expression of Proteins Using COS Cells
- Protein Interaction Assay by Blotting and Band-Shifting Studies
- Fractionation of Nuclear or Cytoplasm Proteins
- Immobilized Protein A
- Pierce IgG (Protein A) Purification Kit
- Protein Purification by GST Binding
- Roche NiNTA protocol
GST-Protein Purification Protocols
ptTS-Neo Vector Information
- Digest I of ptTS-Neo
- Digest II of ptTS-Neo
- ptTS-Neo Vector Information
- Sequence Analysis of ptTS-Neo
Recipes for Solutions
Replicaplating Colonies onto Nitrocellulose Filters
Representational Difference Analysis
RNA Related Protocols
RNA Gel Shift
RNA Isolation
- Controlling Ribonuclease Activity
- DNA Multipreparation
- Electrophoresis of RNA Through Gels Containing Formaldehyde
- Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells
- Gel Electrophoresis of RNA
- Hybridization and Autoradiography
- Isolation of mRNA from Mammalian Cells
- Isolation of mRNA from Suspension of Cells to RNAZol B
- Isolation of Total Cellular RNA
- Isolation of Total RNA from Cultured Cells
- Life Technology Trizol RNA and DNA Isolation
- Nuclease-S1 Mapping of RNA
- RNA Isolation by the RNAZol B Method
- RNAZol B Isolation of RNA
- Selection of Poly(A) RNA
- Staining RNA After Transfer to Nitrocellulose Filters
- Staining RNA Before and After Transfer to Nitrocellulose Filters
- Straight A's mRNA Isolation System
- Transfer of Denatured RNA to Nitrocellulose Filters
- Transfer of Denatured RNA to Nylon Membranes
- Ultraspec-II RNA Isolation System
RNA Preparation from B-Lymphocytes
RT PCR
Sequencing Protocols
Serum-Related Protocols
Southern Blotting Protocols
- 50 mL Rehybridization
- Gene Screen Plus
- Nucleic Acid Transfers for BIOTRANS Nylon Membranes
- PCR Analysis from Rich
- Southern Blots by Lee
- Stripping Probes from Blots
SYBR Green Information
Taq Polymerase Preparation Protocols
- Gene Choice Fact Sheet - Taq DNA Polymerase
- Grimm and Arbuthnot - Rapid Purification of Recombinant Taq
- Guidelines for Use of Robertson Lab Recombinant Taq Polymerase
- Pluthero - Purification of Taq
- Pluthero - Rapid Purification of Recombinant Taq
- Preparation of Taq Polymerase
- Purification of Taq Expressed in E. Coli
- Taq Amplification Using the pAKTaq Plasmid
Transfection Protocols
Transient Transduction with Adenovirus Poly Lysine (AdpL)
Vent DNA Polymerases
- Deep Vent exo DNA Polymerase
- Using Vent DNA Polymerases to Extend a Primer
- Vent DNA Polymerase Technical Data Sheet
Virus Preparation Protocols
- Detection of Proteins in Gels
- Detection of Proteins on Blot Transfer Membranes
- Section IV - Transfer Conditions
- Section V - Specific Protocols
- Section VI - Blotting Membranes
- Stripping and Reprobing – Amersham
- Stripping and Reprobing Membranes in Western Blotting
- Western Blotting - Lysis to Analysis
- Western Blotting
- Competent Yeast Cells
- Direct Transfer of DNA from Yeast to E. Coli by Electroporation
- High Efficiency Yeast Transformation Using RNA as a Carrier
- Improved Method for Transformation of Intact Yeast Cells
- Izumi - Yeast Transformation Transfer to E. Coli
- JAGGED1 and JAGGED2 Sequences
- Protein Interaction Cloning
- Transformation of Yeast Cells
- Yeast Small Scale Transformation